A nano-bio interfacial protein corona on silica nanoparticle

Zhang, Hongyan; Peng, Jiaxi; Li, Xin; Liu, Shengju; Hu, Zhengyan; Xu, Guiju; Wu, Ren’an

doi:10.1016/j.colsurfb.2018.04.021

Cited by 33 publications

(25 citation statements)

References 38 publications

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“…18 In that study, the protein corona, which was formed on superparamagnetic iron oxide nanoparticles, was categorized according to its structure: soft, hard, and tightly bound, which is similar to a more recent study by Zhang et al, where a three-phase protein corona was observed on silica nanoparticles. 50 In that study, the three-phase corona was concluded from protein adsorption at saturation (i.e., post-corona formation). In contrast to the three-layer system, other groups have reported on a two-time-scale dynamic of protein corona formation: an initial strongly bound monolayer and a subsequent weakly bound layer.…”

Section: Mf-clsm Dynamicmentioning

confidence: 99%

In Situ Characterization of Protein Corona Formation on Silica Microparticles Using Confocal Laser Scanning Microscopy Combined with Microfluidics

Weiss

Krüger

Besford

et al. 2019

ACS Appl. Mater. Interfaces

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In biological fluids, proteins bind to particles, forming so-called protein coronas. Such adsorbed protein layers significantly influence the biological interactions of particles, both in vitro and in vivo. The adsorbed protein layer is generally described as a two-component system comprising "hard" and "soft" protein coronas. However, a comprehensive picture regarding protein corona structure is lacking. Herein, we introduce an experimental approach that allows for in situ monitoring of protein adsorption onto silica microparticles. The technique, which mimics flow in vascularized tumors, combines confocal laser scanning microscopy with microfluidics and allows the study of the time-evolution of protein corona formation. Our results show that protein corona formation is kinetically divided into three different phases: phase 1, proteins irreversibly and directly bound (under physiologically relevant conditions) to the particle surface; phase 2, irreversibly bound proteins interacting with pre-adsorbed proteins, and phase 3, reversibly bound "soft" protein corona proteins. Additionally, we investigate particle-protein interactions on lowfouling zwitterionic-coated particles where the adsorption of irreversibly bound proteins does not occur, and on such particles only a "soft" protein corona is formed. The reported approach offers the potential to define new state-of-the art procedures for kinetics and protein fouling experiments. 9 Depending on the characterization method used, the protein corona is described according to either the Gibbs free energy ΔG, 8,10-12 which defines the adsorption and desorption rates of proteins, or binding force 13,14 between the proteins and particle surface. Proteins with a large ΔG have a low probability of desorption and therefore remain associated with the particle surface. These proteins are considered to form the "hard" protein corona. Distinction based on binding forces implies that "hard" protein corona proteins interact directly with the particle surface through long-range, strong protein-surface interactions, whereas proteins in the "soft" protein corona interact with other proteins through short-range, weak protein-protein interactions. Another theoretical distinction is based on the persistence of the protein to remain adsorbed throughout the nanoparticle's journey (i.e. from bloodstream to tissue and past-endocytic environments) as protein corona composition changes during biophysical events. 6-8,15,16 The concept of "persistent" proteins 5 originates from studies where the "hard" protein corona is used to follow the particle's past. 17-19 It is becoming increasingly important to clearly understand the complex process of protein corona formation, with a focus on the influence of the "soft" protein corona on physiological interactions. 4,7,13,20-24 However, to do so, it is crucial to acquire and understand further details such as the time-evolution of protein corona formation. Existing techniques for investigating the protein corona can be divided into ex situ and in situ ...

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Section: Mf-clsm Dynamicmentioning

confidence: 99%

In Situ Characterization of Protein Corona Formation on Silica Microparticles Using Confocal Laser Scanning Microscopy Combined with Microfluidics

Weiss

Krüger

Besford

et al. 2019

ACS Appl. Mater. Interfaces

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“…These particularly tightly bound proteins were referred to as an "interfacial protein corona" in arecent study. [13] However,the question is whether truly significant amounts of protein remains on the surface of every nanocarrier.L ooking at all these aspects,adeeper understanding of the biological impact and importance of the hard as well as the soft protein corona is necessary to proceed in the field of nanomedicine.T herefore,i nt his Minireview,w eg ive an overview about how the sample preparation by commonly established separation methods-that is the separation of nanocarrier-protein complexes from the surrounding medium-influences the outcome of the protein corona analysis.Special focus was placed on the requirements and limitations of the sample preparation process itself as the different characterization techniques used subsequently have already been reviewed extensively. [8,12,[14][15][16] One of the biggest challenges in the field of nanomedicine is the adsorption of biomolecules on the nanomaterial upon contact with abiological medium.…”

Section: Introductionmentioning

confidence: 99%

Possibilities and Limitations of Different Separation Techniques for the Analysis of the Protein Corona

2019

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One of the biggest challenges in the field of nanomedicine is the adsorption of biomolecules on the nanomaterial upon contact with a biological medium. The interactions of the resulting protein corona are essential for their behavior in a biological system. Thus, it is now commonly accepted that understanding the formation and consequently understanding the influence of the protein corona on the biological response is crucial. However, the outcome of the protein corona characterization cannot easily be compared between different studies and techniques, since many different sample preparation procedures exist that are suitable for different materials or methods. Depending on the applied procedure, the nanomaterial–protein system will be altered in a certain way, so that it is necessary to consider the individual influence on the protein corona. Accordingly, the aim of this Minireview is to give an overview of the applied sample preparation methods for the analysis of the protein corona and to evaluate their influence on the outcome of the results especially with regard to the introduced terms “soft” and “hard protein corona”. Special focus will be placed on the comparison of the most commonly used techniques such as centrifugation, magnetic, and chromatographic separation.

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“…This study is based on the premise that NP surface functionalisation may affect biomolecule (especially protein) corona formation [18], which can in turn influence mechanisms that are involved in nano-based antibiofilm processes such as NP aggregation, bacterial adhesion, and NP uptake. Here, Pseudomonas fluorescens biofilms were grown at the air-liquid interface and had their EPS extracted using a cation exchange resin to weaken the overall matrix architecture, which is maintained by Ca 2+ and Mg 2+ bridges [19,20].…”

Section: Introductionmentioning

confidence: 99%

Interactions between functionalised silica nanoparticles and Pseudomonas fluorescens biofilm matrix: A focus on the protein corona

et al. 2020

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Biofilms are microbial communities embedded in an extracellular polymeric matrix and display an enhanced tolerance to the action of antimicrobials. The emergence of novel functionalised nanoparticles is considered a promising avenue for the development of biofilmspecific antimicrobial technologies. However, there is a gap in the understanding of interactions between nanoparticles and the biofilm matrix. Particularly, questions are raised on how nanoparticle charge and surface groups play a role in aggregation when in contact with biofilm components. Herein we present the synthesis of four types of silica nanoparticles and undertake an analysis of their interactions with Pseudomonas fluorescens biofilm matrix. The effect of the biofilm matrix components on the charge and aggregation of the nanoparticles was assessed. Additionally, the study focused on the role of matrix proteins, with the in-depth characterisation of the protein corona of each nanoparticle by Liquid Chromatography with Tandem Mass Spectrometry experiments. The protein corona composition is dependent on the nanoparticle type; non-functionalised nanoparticles show less protein selectivity, whereas carboxylate-functionalised nanoparticles prefer proteins with a higher isoelectric point. These outcomes provide insights into the field of biofilm-nanoparticle interactions that can be valuable for the design of new nano-based targeting systems in future anti-biofilm applications.

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A nano-bio interfacial protein corona on silica nanoparticle

Cited by 33 publications

References 38 publications

In Situ Characterization of Protein Corona Formation on Silica Microparticles Using Confocal Laser Scanning Microscopy Combined with Microfluidics

In Situ Characterization of Protein Corona Formation on Silica Microparticles Using Confocal Laser Scanning Microscopy Combined with Microfluidics

Possibilities and Limitations of Different Separation Techniques for the Analysis of the Protein Corona

Interactions between functionalised silica nanoparticles and Pseudomonas fluorescens biofilm matrix: A focus on the protein corona

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