A NADH-dependent fiber-optic biosensor for ethanol determination with a UV-LED excitation system

Kudo, Hiroyuki; Sawai, Masayuki; Wang, Xin; Gessei, Tomoko; Koshida, Tomoyuki; Miyajima, Koichiro; Saito, Hirokazu; Mitsubayashi, Kohji

doi:10.1016/j.snb.2009.06.008

Cited by 47 publications

(30 citation statements)

References 39 publications

(39 reference statements)

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“…The activity of ADH used for this biosensor (Yeast ADH) is most active with ethanol and the activity decreases as the size of the alcohol increases. Thus, the biosensor s showed 30 times higher output for ethanol than that of methanol [9]. According to the result, the fiber-optic biosensor for ethanol solution was considered to be useful for gas monitoring use.…”

Section: Fiber Optic Bio-sniffermentioning

confidence: 96%

“…For its low power consumption, UV-LEDs are suitable for laptop applications such as on-site monitoring. Therefore, we applied a UV-LED (λ=340nm) as an excitation source of NADH fluorometric biosensor in the previous study [9]. Continuous alcohol gas monitoring with simplified measurement system can be expected by applying such a fluorometric biosensing system to the gas phase.…”

Section: Introductionmentioning

confidence: 99%

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Fiber optic bio-sniffer (biochemical gas sensor) using UV-LED light for monitoring ethanol vapor with high sensitivity & selectivitiy

et al. 2009

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A fiber optic bio-sniffer (biochemical gas sensor) for alcohol gas monitoring with high sensitivity and high selectivity was fabricated and tested. The bio-sniffer is a gas sensor that uses molecular recognition of enzyme to improve selectivity. Usually, enzyme loses activity in the gas phase. Applying a flowcell with a gas-intake window to the sensing probe, enzyme immobilized at the sensing region was kept in the sufficient wet condition to maintain activity. The bio-sniffer measures ethanol (EtOH) vapor by measuring fluorescence of nicotinamide adenine dinucleotide (NADH), which is produced by enzymatic reaction at the flow-cell. In order to construct a simplified system suitable for on-site applications, a high-intensity ultraviolet light emitting diode (UV-LED) was utilized as an excitation light. Owing to low power consumption comparing with previous light sources, the bio-sniffer was considered to be suitable for laptop applications such as on-site monitoring. According to the characterization, the bio-sniffer for was useful for continuous alcohol monitoring and showed high selectivity. The calibration range was 0.30-300 ppm which is suitable for evaluation of capacity to metabolize alcohol.

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Section: Fiber Optic Bio-sniffermentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Fiber optic bio-sniffer (biochemical gas sensor) using UV-LED light for monitoring ethanol vapor with high sensitivity & selectivitiy

et al. 2009

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“…(27,28) Briefly, FALDH/PB solution was mixed with PMEH/ethanol solution so that eventually their concentrations were 50 units•cm −2 and 1 mL•cm −2 on the membrane, respectively. Afterwards, the H-PTFE membrane was coated with the mixed solution, followed by curing in a refrigerator at 4 °C for 180 min.…”

Section: Construction Of the Bio-sniffermentioning

confidence: 99%

Direct Measurement of Gaseous Formaldehyde from Food with a Fiber-Optic Biochemical Gas Sensor (Bio-sniffer)

Toma¹,

Miyajima²,

Shin‐ichi³

et al. 2016

Sensors and Materials

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In this work, we assessed the ability of a fiber-optic biochemical gas sensor (bio-sniffer) to directly and nondestructively measure gaseous formaldehyde (FA) released from food. In the biosniffer, formaldehyde dehydrogenase (FALDH) was immobilized in a hydrophilic fluoropolymer membrane in which an enzymatic reaction producing nicotinamide adenine dinucleotide (NADH) occurred. The resultant NADH was excited with ultraviolet (UV) light irradiated from the optical fiber end in the bio-sniffer and the emitted fluorescence was coupled back to the fiber end and detected. In experiments to characterize the bio-sniffer using standard gaseous FA samples, the capability of continuous measurement (a coefficient of variation of 3.4% for five successive measurements) and high sensitivity (a limit of detection of 1.1 ppb) were validated. Then a demonstration of direct gaseous FA from food was carried out using Shiitake mushrooms (Lentinula edodes). Owing to the use of the enzyme, the bio-sniffer was able to selectively measure FA, and clear differences in FA concentrations were observed depending on the state of the mushrooms, i.e., dried, rehydrated with deionized water or with γ-GTPase inhibitor. These results indicated that the bio-sniffer allowed the direct monitoring of FA naturally produced in food or the evaluation of the metabolism of living organisms.

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“…Enzyme based Enzymes are chosen in this biosensor in such a way that the reactions couple the target analyte to form a basis of detection. Prakash et al (2008) (Kudo et al 2009). Zhong et al (2011) built an enzymatic biosensor using toluene orthomono oxygenase to detect toluene.…”

Section: Classification Of Fiber Optic Biosensorsmentioning

confidence: 99%

Optical biosensors for food quality and safety assurance—a review

et al. 2011

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Food quality and safety is a scientific discipline describing handling, preparation and storage of food in ways that prevent food borne illness. Food serves as a growth medium for microorganisms that can be pathogenic or cause food spoilage. Therefore, it is imperative to have stringent laws and standards for the preparation, packaging and transportation of food. The conventional methods for detection of food contamination based on culturing, colony counting, chromatography and immunoassay are tedious and time consuming while biosensors have overcome some of these disadvantages. There is growing interest in biosensors due to high specificity, convenience and quick response. Optical biosensors show greater potential for the detection of pathogens, pesticide and drug residues, hygiene monitoring, heavy metals and other toxic substances in the food to check whether it is safe for consumption or not. This review focuses on optical biosensors, the recent developments in the associated instrumentation with emphasis on fiber optic and surface plasmon resonance (SPR) based biosensors for detecting a range of analytes in food samples, the major advantages and challenges associated with optical biosensors. It also briefly covers the different methods employed for the immobilization of bio-molecules used in developing biosensors.

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A NADH-dependent fiber-optic biosensor for ethanol determination with a UV-LED excitation system

Cited by 47 publications

References 39 publications

Fiber optic bio-sniffer (biochemical gas sensor) using UV-LED light for monitoring ethanol vapor with high sensitivity & selectivitiy

Fiber optic bio-sniffer (biochemical gas sensor) using UV-LED light for monitoring ethanol vapor with high sensitivity & selectivitiy

Direct Measurement of Gaseous Formaldehyde from Food with a Fiber-Optic Biochemical Gas Sensor (Bio-sniffer)

Optical biosensors for food quality and safety assurance—a review

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A NADH-dependent fiber-optic biosensor for ethanol determination with a UV-LED excitation system

Cited by 47 publications

References 39 publications

Fiber optic bio-sniffer (biochemical gas sensor) using UV-LED light for monitoring ethanol vapor with high sensitivity &#x00026; selectivitiy

Fiber optic bio-sniffer (biochemical gas sensor) using UV-LED light for monitoring ethanol vapor with high sensitivity &#x00026; selectivitiy

Direct Measurement of Gaseous Formaldehyde from Food with a Fiber-Optic Biochemical Gas Sensor (Bio-sniffer)

Optical biosensors for food quality and safety assurance—a review

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Fiber optic bio-sniffer (biochemical gas sensor) using UV-LED light for monitoring ethanol vapor with high sensitivity & selectivitiy

Fiber optic bio-sniffer (biochemical gas sensor) using UV-LED light for monitoring ethanol vapor with high sensitivity & selectivitiy