A Mutational Analysis of the Transforming Functions of the E8 Protein of Bovine Papillomavirus Type 4

O'Brien, Vincent; Ashrafi, G.Hossein; Grindlay, G.Joan; Anderson, Ruth A.; Campo, M.Saveria

doi:10.1006/viro.1999.9784

Cited by 5 publications

(14 citation statements)

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“…BPV-1 infects fibroblasts and skin keratinocytes giving rise to fibropapillomas, whereas BPV-4 infects solely the epithelial cells of the mucous epithelium of the upper gastrointestinal tract [ 132 ]. Thus, while expression of BPV-1 E5 by itself is sufficient to fully transform mouse and primary human fibroblast cells [ 133 , 134 ], BPV-4 E5 contributes to the transformed phenotype of primary cells by conferring anchorage independent growth, growth in low serum, focus formation and increased cell motility but only in the presence of the other viral oncoproteins, resembling in this respect 16E5 (Table 1 ) [ 127 , 135 , 136 ]. For both proteins the hydrophobic domain, the hydrophilic residues at position 17 and the hydrophilic C-terminal tail are critical for their transforming activities [ 135 , 137 ].…”

Section: Bpv E5mentioning

confidence: 99%

“…Unlike BPV-1 E5, BPV-4 E5 induces transformation of established cells independently from the constitutive activation of tyrosine kinase growth receptors (Table 1 ) [ 165 ]. Cell transformation by BPV-4 E5 (either of established cells by itself or of primary cells in co-operation with other viral oncoproteins) is achieved through transactivation of the cyclin A gene promoter, increased cyclin A expression and cyclin A-associated kinase activity, and inhibition of the negative regulator of cell cycle, p27 Kip1 [ 135 , 166 - 168 ]. Mutational analyses of BPV-4 E5 have demonstrate that, as is the case for BPV-1 E5, the residue at position 17 and the hydrophilic C-terminal tail are critical for its transforming activities [ 135 ].…”

Section: Bpv E5mentioning

confidence: 99%

“…Cell transformation by BPV-4 E5 (either of established cells by itself or of primary cells in co-operation with other viral oncoproteins) is achieved through transactivation of the cyclin A gene promoter, increased cyclin A expression and cyclin A-associated kinase activity, and inhibition of the negative regulator of cell cycle, p27 Kip1 [ 135 , 166 - 168 ]. Mutational analyses of BPV-4 E5 have demonstrate that, as is the case for BPV-1 E5, the residue at position 17 and the hydrophilic C-terminal tail are critical for its transforming activities [ 135 ]. Thus both mutation of Asp 17 and deletion of the C-terminus hydrophilic domain completely abrogate cell transformation and E5 ability to activate the cyclin A pathway [ 135 ].…”

Section: Bpv E5mentioning

confidence: 99%

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Papillomavirus E5: the smallest oncoprotein with many functions

Venuti¹,

Paolini²,

et al. 2011

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Papillomaviruses (PVs) are established agents of human and animal cancers. They infect cutaneous and mucous epithelia. High Risk (HR) Human PVs (HPVs) are consistently associated with cancer of the uterine cervix, but are also involved in the etiopathogenesis of other cancer types. The early oncoproteins of PVs: E5, E6 and E7 are known to contribute to tumour progression. While the oncogenic activities of E6 and E7 are well characterised, the role of E5 is still rather nebulous. The widespread causal association of PVs with cancer makes their study worthwhile not only in humans but also in animal model systems. The Bovine PV (BPV) system has been the most useful animal model in understanding the oncogenic potential of PVs due to the pivotal role of its E5 oncoprotein in cell transformation. This review will highlight the differences between HPV-16 E5 (16E5) and E5 from other PVs, primarily from BPV. It will discuss the targeting of E5 as a possible therapeutic agent.

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Section: Bpv E5mentioning

confidence: 99%

Section: Bpv E5mentioning

confidence: 99%

Section: Bpv E5mentioning

confidence: 99%

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Papillomavirus E5: the smallest oncoprotein with many functions

Venuti¹,

Paolini²,

et al. 2011

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“…Moreover, E5 activates several protein kinases involved in the control of cell cycle, thus causing a general dysregulation of the normally tightly controlled programme of cell proliferation. [35][36][37] E5 and MHC class I down-regulation The major histocompatibility complex class I (MHC I) plays a critical role in immune surveillance as it is responsible for the presentation of antigenic peptides to effector T cells. The complex consists of heavy chain (HC), β 2-microglobulin and peptide, and is transported from the endoplasmic reticulum through the Golgi apparatus to the plasma membrane.…”

Section: E5 Oncoproteinmentioning

confidence: 99%

Bovine papillomaviruses: their role in the aetiology of cutaneous tumours of bovids and equids

Nasir

Campo

2008

Veterinary Dermatology

200

197

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Bovine papillomavirus (BPV) is perhaps the most extensively studied animal papillomavirus. In cattle BPVs induce benign tumours of cutaneous or mucosal epithelia, called papillomas or warts. Cattle papillomas are benign tumours and generally regress without eliciting any serious clinical problems in the host, but occasionally persist and provide the focus for malignant transformation to squamous cell carcinoma, as in the case of cancer of the urinary bladder and cancer of the upper alimentary canal. BPV is the only papillomavirus that jumps species: the virus also infects equids, and gives rise to fibroblastic tumours called sarcoids. Sarcoids very rarely regress, more often they persist and can be locally aggressive. These tumours are the most common dermatological tumour of equids worldwide. The purpose of this review is to discuss the biology of BPV, the biology of bovine tumours and equine sarcoids, and present the current understanding of BPV in tumour pathogenesis in its natural host, cattle, and in its heterologous host, equids. Finally, the use of anti-BPV vaccines as a therapy for equine sarcoids will be discussed. Only limited information on the clinical or pathological aspects of either bovine or equine tumours will be provided as this subject has been extensively addressed previously.

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“…BPV-3, -4 and -6 lack the E6 ORF and contain an E8 or E5 ORF (formerly E8 ORF) in the position of the E6 ORF. The BPV-4 E5 ORF consists of 42 aa, induces anchorage-independent growth of infected cells and suppresses contact inhibition (O'Brien et al, 1999;Morgan & Campo, 2000).…”

mentioning

confidence: 99%

Complete genome and phylogenetic position of bovine papillomavirus type 7

Ogawa

Tomita

Okada

et al. 2007

Journal of General Virology

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Six bovine papillomavirus (BPV) types and 16 putative BPV types have been reported previously. Here, the complete genome sequence of BAPV6, a novel putative BPV type isolated from cattle in Japan, was determined by using multiple-primed rolling-circle amplification. The genome consisted of 7412 bp (G+C content of 46 mol%) that encoded five early (E1, E2, E4, E6 and E7) and two late (L1 and L2) genes, but did not encode the E5 gene. The E6 protein contained a non-consensus CxxC(x)33CxxC and a consensus CxxC(x)29CxxC zinc-binding domain, and the E7 protein lacked the LxCxE motif. The nucleotide sequence of the L1 open reading frame (ORF) was related most closely (57–58 %) to the L1 ORF of member(s) of the genera Betapapillomavirus, Gammapapillomavirus and Pipapillomavirus. Phylogenetic analysis based on the complete L1 ORF suggests that BAPV6 should be classified in a novel genus in the family Papillomaviridae as BPV-7.

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A Mutational Analysis of the Transforming Functions of the E8 Protein of Bovine Papillomavirus Type 4

Cited by 5 publications

References 0 publications

Papillomavirus E5: the smallest oncoprotein with many functions

Papillomavirus E5: the smallest oncoprotein with many functions

Bovine papillomaviruses: their role in the aetiology of cutaneous tumours of bovids and equids

Complete genome and phylogenetic position of bovine papillomavirus type 7

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