A mutation in CsABCB19 encoding an ATP-binding cassette auxin transporter leads to erect and compact leaf architecture in cucumber (Cucumis sativus L.)

Cheng, Feng; Song, Mengfei; Zhang, Mengru; Zha, Gaohui; Yin, Juan; Chen, Cheng; Chen, Jinfeng; Lou, Qunfeng

doi:10.1016/j.plantsci.2023.111625

Cited by 3 publications

(3 citation statements)

References 39 publications

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“…Quantitative data showed that the average LPA and average leaf area decreased by 24.5% and 46.6%, respectively, in the cpa1 mutant compared to that of the WT in the middle internodes (7th–12th internodes) 40 days after planting (DAP) (Figure 1d,e). The cell size ratio of the petiole base between the abaxial and adaxial sides determines the LPA of cucumber (Chen et al ., 2022; Cheng et al ., 2023). To investigate whether CPA1 regulates LPA through cell expansion, we compared the cross‐section of the petiole base between cpa1 mutant and WT plants.…”

Section: Resultsmentioning

confidence: 99%

“…Most genes that affect a trait often also have some other effects, which in most cases are detrimental (Cheng et al ., 2023; Liu et al ., 2019; Wang et al ., 2020; Zhang et al ., 2021). However, studies have shown that some genes exist that specifically alter certain traits or mediate multiple beneficial effects.…”

Section: Discussionmentioning

confidence: 99%

“…Over the past 10 years, several key genes regulating plant architectural traits, such as branching, LPA, and plant height, have been identified in cucumber (Cheng et al ., 2023; Shen et al ., 2019; Wang et al ., 2022). However, genes that simultaneously regulate the LPA and leaf size have not been identified.…”

Section: Introductionmentioning

confidence: 99%

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CsHLS1‐CsSCL28 module regulates compact plant architecture in cucumber

Wang,

Li,

Fang

et al. 2024

Plant Biotechnology Journal

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SummaryIncreased planting densities boost crop yields. A compact plant architecture facilitates dense planting. However, the mechanisms regulating compact plant architecture in cucurbits remain unclear. In this study, we identified a cucumber (Cucumis sativus) compact plant architecture (cpa1) mutant from an ethyl methane sulfonate (EMS)‐mutagenized library that exhibited distinctive phenotypic traits, including reduced leaf petiole angle and leaf size. The candidate mutation causes a premature stop codon in CsaV3_1G036420, which shares similarity to Arabidopsis HOOKLESS 1 (HLS1) encoding putative histone N‐acetyltransferase (HAT) protein and was named CsHLS1. Consistent with the mutant phenotype, CsHLS1 was predominantly expressed in leaf petiole bases and leaves. Constitutive overexpressing CsHLS1 in cpa1 restored the wild‐type plant architecture. Knockout of CsHLS1 resulted in reduces leaf petiole angle and leaf size and as well as decreased acetylation levels. Furthermore, CsHLS1 directly interacted with CsSCL28 and negatively regulated compact plant architecture in cucumber. Importantly, CsHLS1 knockout increased the photosynthesis rate and leaf nitrogen in cucumbers, thereby maintaining cucumber yield at normal density. Overall, our research provides valuable genetic breeding resource and gene target for creating a compact plant architecture for dense cucumber planting.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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CsHLS1‐CsSCL28 module regulates compact plant architecture in cucumber

Wang,

Li,

Fang

et al. 2024

Plant Biotechnology Journal

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Major quantitative trait locus qLA3.1 is related to tomato leaf angle by regulating cell length at the petiole base

Wang,

Zhang

et al. 2024

Theor Appl Genet

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Mining Candidate Genes for Leaf Angle in Brassica napus L. by Combining QTL Mapping and RNA Sequencing Analysis

Peng,

Li,

Wang

et al. 2024

IJMS

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Leaf angle (LA) is an important trait of plant architecture, and individuals with narrow LA can better capture canopy light under high-density planting, which is beneficial for increasing the overall yield per unit area. To study the genetic basis and molecular regulation mechanism of leaf angle in rapeseed, we carried out a series of experiments. Quantitative trait loci (QTL) mapping was performed using the RIL population, and seven QTLs were identified. Transcriptome analysis showed that the cell wall formation/biogenesis processes and biosynthesis/metabolism of cell wall components were the most enrichment classes. Most differentially expressed genes (DEGs) involved in the synthesis of lignin, xylan, and cellulose showed down-regulated expression in narrow leaf material. Microscopic analysis suggested that the cell size affected by the cell wall in the junction area of the stem and petiole was the main factor in leaf petiole angle (LPA) differences. Combining QTL mapping and RNA sequencing, five promising candidate genes BnaA01G0125600ZS, BnaA01G0135700ZS, BnaA01G0154600ZS, BnaA10G0154200ZS, and BnaC03G0294200ZS were identified in rapeseed, and most of them were involved in cell wall biogenesis and the synthesis/metabolism of cell wall components. The results of QTL, transcriptome analysis, and cytological analysis were highly consistent, collectively revealing that genes related to cell wall function played a crucial role in regulating the LA trait in rapeseed. The study provides further insights into LA traits, and the discovery of new QTLs and candidate genes is highly beneficial for genetic improvement.

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A mutation in CsABCB19 encoding an ATP-binding cassette auxin transporter leads to erect and compact leaf architecture in cucumber (Cucumis sativus L.)

Cited by 3 publications

References 39 publications

CsHLS1‐CsSCL28 module regulates compact plant architecture in cucumber

CsHLS1‐CsSCL28 module regulates compact plant architecture in cucumber

Major quantitative trait locus qLA3.1 is related to tomato leaf angle by regulating cell length at the petiole base

Mining Candidate Genes for Leaf Angle in Brassica napus L. by Combining QTL Mapping and RNA Sequencing Analysis

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