A murine skeletal muscle ischemia-reperfusion injury model: differential pathology in BALB/c and DBA/2N mice

Abstract

Ischemia-reperfusion injuries can occur with diseases such as myocardial infarction and stroke and during surgical procedures such as organ transplantation and correction of aortic aneurysms. We developed a murine model to mimic abdominal aortic aneurysm repair with cross-clamping of the aorta distal to the renal artery. After model development, we compared the normal complement BALB/c mouse with the C5-deficient DBA/2N mouse. To assess quantitative differences, we measured neuromuscular function up to 72 h after ischemia with a subjective clinical scoring system, as well as plasma chemistries, hematology, and histopathology. There were significant increases in clinical scores and creatine phosphokinase, lactate dehydrogenase, and muscle histopathology scores in BALB/c mice compared with those in DBA/2N mice and sham-surgery mice. Muscle histopathology scores of the cranial tibialis and quadriceps correlated well with clinical signs, creatine phosphokinase, and lactate dehydrogenase, and indicated the greatest pathology in these muscle groups. We developed a murine model of skeletal muscle ischemia-reperfusion injury that can utilize the benefits of murine genetic and transgenic models to assess therapeutic principles of this model. Additionally, we have shown a significant reduction in clinical signs, plasma muscle enzyme concentrations, and muscle pathology in the C5-deficient DBA/2N mouse in this model.

Local and Remote Ischemia-Reperfusion Injury Is Mitigated in Mice Overexpressing Human C1 Inhibitor

“…We chose a severe lower torso ischemia-reperfusion injury model [21,25]. While the mortality of 105 min of ischemia followed by reperfusion has been reported to be between 10 [23, 25] and 57% in mice [21], all animals of our series survived 120 min of ischemia and 180 min of reperfusion (n = 12).…”

Dynamic force responses of skeletal muscle during stretch–shortening cycles

“…Digital pictures were obtained using an Olympus (Model AX70) microscope and a Quantix digital imaging system (Photometrics, Tucson, Ariz.). Morphologic assessment by a pathologist was selected for evaluation of the sections because it is an established method for evaluating muscle damage (Carter et al 1998;Helliwell 1999) and because it allows assessment of injury across the entire muscle when swollen, atrophic, and necrotic fibers are all present in the same section. Frozen sections of tibialis anterior muscle were examined by a board-certified veterinary pathologist blinded to the exposure status of the rats.…”

Protective effects of a potent c5a receptor antagonist on experimental acute limb ischemia-reperfusion in rats

“…Lower limb ischemia-reperfusion (I/R) injury is a serious problem following the surgical repair of abdominal aortic aneurysm as well as traumatic crush injuries [1,2]. Ischemia and the subsequent reperfusion of the skeletal muscle tissue stimulates an inflammatory response in the affected muscle as well as inducing injury in other tissues [3].…”