A murine retrovirus induces proliferation of unique lymphoid cell lines expressing T-cell-receptor structures utilizing common variable region alpha and beta chain genes.

Abstract

A murine radiation leukemia virus (RadLV) has been shown to induce in vitro proliferation of an unusual subset of lymphoid cells from spleen. They have the unusual property of expressing CD3/T-cell receptor a and ,B chains (TCR-a,l) in the absence ofother T-cell markers such as Thy-i, CD4, and CD8. Cell lines induced in two mouse strains with RadLV produced by the C6VL/1 thymoma all specifically utilize common Va3 and V.88.2 variable region genes in the formation of a TCR structure. Each of these cell lines has now been found to express both class I and dass II major histo-

The Lineage Relationship of Dendritic Cells with Other Haematopoietic Cells

“…Other evidence indicates that D cell precursors share phenotypic characteristics with lymphoid precursors present in thymus and can be isolated by cell sorting along with lymphoid precursor cells [20,21]. The isolation of transformed cell lines expressing some T cell-specific markers as well as functional properties resembhng D cells also supports a lineage relationship between D cells and early lymphoid precursors [22,23].…”

“…A T-cell-tropic murine retrovirus has been shown to immortalize a subset of lymphoid cells in spleen [22] which display some properties resembling D cells. Cell lines display lineage infidelity in that they express the B220 (CD45) marker of B and pre B cells and the NK 1.1 marker of natural killer cells as well as components of the T-cell receptor/CD3 complex [22,23]. They do not express any myeioid cell markers, nor has it been possible to induce expression of specific markers of myeioid cells by various means of stimulation.…”

“…They appear to have undergone restricted differentiation reflecting lymphoid and D-cell properties. Some cell lines have been found to have an antigen-presenting function [22], capacity to migrate to thymus [30], expression of class-II antigens and the CDllc common to D cells and all can endocytose protein [23]. One of these cell hnes has been shown to adopt expression of the Tcell markers Thy-1, CD4 and CD8 upon intrathymic transfer and replication in mice [31].…”