A Murine Osteosarcoma Cell Line with a Potential to Develop Ossification upon Transplantation

Kusumi, Tomomi; Nishi, Takashi; Tanaka, Masanori; Tsuchida, Shigeki; Kudo, Hiroto

doi:10.1111/j.1349-7006.2001.tb01144.x

Cited by 9 publications

(6 citation statements)

References 44 publications

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“…OPN is unlikely associated with hard tissue formation of the salivary gland tumors. On the other hand, OPN is frequently expressed in osteosarcoma (23). Total absence of OPN expression may support the salivary gland origin of the present case.…”

Section: Discussionsupporting

confidence: 72%

Expression of Bone Matrix Proteins in Malignant Myoepithelioma with Extensive Osteoid Formation Occurring in The Maxilla.

et al. 2003

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Section: Discussionsupporting

confidence: 72%

Expression of Bone Matrix Proteins in Malignant Myoepithelioma with Extensive Osteoid Formation Occurring in The Maxilla.

et al. 2003

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“…Osteosarcoma cell lines have been established from human, dog and rat, and these cell lines have the ability to undergo ossification after xenotransplantation [13,16,21,29,31]. Thus the cell lines are useful for studies of the mechanism of ossification as well as the relationship between BMPs and metastasis of osteosarcoma.…”

mentioning

confidence: 99%

Establishment and Characterization of a Cell Line, MCO-Y4, Derived from Canine Mammary Gland Osteosarcoma

Kawabata

Yamamoto

Lan

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. A cell line, MCO-Y4, was established from a mammary gland osteosarcoma of a 16-year-old female mongrel dog. Histopathologically the tumor was composed of osteoblastic cells with an osteoid meshwork and chondroid matrix. The mean doubling time of the cells at the 93rd passage was 32.39 ± 4.66 hr. Immunohistochemically, the osteoblastic and chondroblastic cells were positive for bone morphogenetic protein (BMP)-2/4 and BMP receptor (BMPR) II. The cultured cells were spindle in shape during the growth and the confluent phases. No tumor matrix was detected in the culture dish by alcian blue staining or von-Kossa silver impregnation. MCO-Y4 cells on the chamber slides showed intense immunoreactivity for BMP-2/4 and BMPR II. Noggin, an antagonist for BMP-2/ 4, showed the growth inhibition on MCO-Y4 cells. In addition, fibronectin might be potential for stimulating growth of MCO-Y4 cells. When transplanted into severe combined immunodeficiency mice, the cells formed tumors consisting of solid proliferation of osteoblastic and fibroblastic cells with woven-bone trabeculae. These tumor cells were intensely positive for BMP-2/4 and BMPR II. Our results suggested that the cell line might be useful for studying the role of BMPs in canine osteosarcoma and the mechanism of ossification. KEY WORDS: bone morphogenetic protein, canine mammary gland, cell line, noggin, osteosarcoma.

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“…In fact, in most OS, there are also chondrocytes and fibroblasts that present in the matrix. It has been reported that there is cartilage matrix in the tumor tissues of mice transplanted with OS cell lines, and it has ossification similar to the process of endochondral ossification, suggesting that chondrogenesis or endochondral ossification plays a role in the development of OS [19,20]. RUNX2 is a transcription factor belonging to the Runt domain gene family, which is to play a crucial role in osteoblast differentiation and chondrocyte maturation [39,114,115].…”

Section: Chemical Induction Modelsmentioning

confidence: 99%

“…Chondrocytes and fibroblasts are also present in the tumor stroma of many osteosarcomas. In addition, in the induced mouse OS model, cartilage matrix was found present in the tumor tissue and ossification occurred through similar process of endochondral ossification [19,20]. As a transcription factor, RUNX2 is known to play a crucial role in osteoblast differentiation and chondrocyte hypertrophy or maturation.…”

Section: Introductionmentioning

confidence: 99%

Collagen type X expression and chondrocyte hypertrophic differentiation during OA and OS development

Han

2024

Am J Cancer Res

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Chondrocyte hypertrophy and the expression of its specific marker, the collagen type X gene (COL10A1), constitute key terminal differentiation stages during endochondral ossification in long bone development. Mutations in the COL10A1 gene are known to cause schmid type metaphyseal chondrodysplasia (SMCD) and spondyloepiphyseal dyschondrodysplasia (SMD). Moreover, abnormal COL10A1 expression and aberrant chondrocyte hypertrophy are strongly correlated with skeletal diseases, notably osteoarthritis (OA) and osteosarcoma (OS). Throughout the progression of OA, articular chondrocytes undergo substantial changes in gene expression and phenotype, including a transition to a hypertrophic-like state characterized by the expression of collagen type X, matrix metalloproteinase-13, and alkaline phosphatase. This state is similar to the process of endochondral ossification during cartilage development. OS, the most common pediatric bone cancer, exhibits characteristics of abnormal bone formation alongside the presence of tumor tissue containing cartilaginous components. This observation suggests a potential role for chondrogenesis in the development of OS. A deeper understanding of the shifts in collagen X expression and chondrocyte hypertrophy phenotypes in OA or OS may offer novel insights into their pathogenesis, thereby paving the way for potential therapeutic interventions. This review systematically summarizes the findings from multiple OA models (e.g., transgenic, surgically-induced, mechanically-loaded, and chemically-induced OA models), with a particular focus on their chondrogenic and/or hypertrophic phenotypes and possible signaling pathways. The OS phenotypes and pathogenesis in relation to chondrogenesis, collagen X expression, chondrocyte (hypertrophic) differentiation, and their regulatory mechanisms were also discussed. Together, this review provides novel insights into OA and OS therapeutics, possibly by intervening the process of abnormal endochondral-like pathway with altered collagen type X expression.

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A Murine Osteosarcoma Cell Line with a Potential to Develop Ossification upon Transplantation

Cited by 9 publications

References 44 publications

Expression of Bone Matrix Proteins in Malignant Myoepithelioma with Extensive Osteoid Formation Occurring in The Maxilla.

Expression of Bone Matrix Proteins in Malignant Myoepithelioma with Extensive Osteoid Formation Occurring in The Maxilla.

Establishment and Characterization of a Cell Line, MCO-Y4, Derived from Canine Mammary Gland Osteosarcoma

Collagen type X expression and chondrocyte hypertrophic differentiation during OA and OS development

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