A murine model of acute lung injury identifies growth factors to promote tissue repair and their biomarkers

Abstract

Type II alveolar epithelial cells (AEC2s) play a crucial role in the regeneration of type I AECs after acute lung injury. The mechanisms underlying the regeneration of AEC2s are not fully understood. To address this issue, here, we investigated a murine model of acute lung injury using mice expressing human Diphtheria Toxin Receptor (DTR) under the control of Lysozyme M promoter (LysM‐DTR). DT injection induced the depletion of AEC2s, alveolar macrophages, and bone marrow (BM)‐derived myeloid cells in LysM‐DTR mice, and the mice died within 6 days after DT injection. Apoptotic AEC2s and bronchiolar epithelial cells appeared at 24 hr, whereas Ki67‐positive proliferating cells appeared in the alveoli and bronchioles in the lung of LysM‐DTR mice at 72–96 hr after DT injection. Transfer of wild‐type BM cells into LysM‐DTR mice accelerated the regeneration of AEC2s along with the up‐regulation of several growth factors. Moreover, several metabolites were significantly decreased in the sera of LysM‐DTR mice compared with WT mice after DT injection, suggesting that these metabolites might be biomarkers to predict AEC2s injury. Together, LysM‐DTR mice might be useful to identify growth factors to promote lung repair and the metabolites to predict the severity of lung injury.

A comprehensive exploration of hydrogel applications in multi-stage skin wound healing

“…To stimulate cell proliferation and promote tissue repair, the fibroblast growth factor, EGF, and platelet-derived growth factor are essential components. [153][154][155] Kang et al developed a hydrogel that reduces scar by activating growth factors associated with remodeling of the ECM. 156 The ECM creates a complex milieu that is essential for wound protection and healing.…”

Mechanism of Alleviating Acute Lung Injury in Mice from Serum Metabolomics Analysis of Cordyceps fumosorosea

“…Pulmonary infiltrates involve abnormal substances such as fluid, cells or inflammatory debris in the lung tissue or airspaces. ALI, on the other hand, is a severe respiratory condition triggered by various factors, including pneumonia, sepsis, burns, trauma and systemic inflammatory reactions rooted in diffuse lung cell injuries (DLCI) [5]. ALI is characterized by lung tissue damage and intense oxidative stress caused by pathogens, chemicals and trauma, making it a serious and potentially life-threatening condition [6].…”

Mechanism of alleviating acute lung injury in mice from serum metabolomics analysis of Cordyceps fumosorosea

“…Pulmonary in ltrates involve abnormal substances such as uid, cells, or in ammatory debris in the lung tissue or airspaces. ALI, on the other hand, is a severe respiratory condition triggered by various factors, including pneumonia, sepsis, burns, trauma, and systemic in ammatory reactions rooted in diffuse lung cell injuries (DLCI) (Kurosawa et al, 2019). ALI is characterized by lung tissue damage and intense oxidative stress caused by pathogens, chemicals, and trauma, making it a serious and potentially life-threatening condition (LV, 2016).…”