A multiwell plate Organ-on-Chip (OOC) device for in-vitro cell culture stimulation and monitoring

Gaio, Nikolas; Waafi, Affan Kaysa; Vlaming, Maria L. H.; Boschman, E.; Dijkstra, Paul; Nacken, Peter; Braam, Stefan; Boucsein, Clemens; Sarro, P.M.; Dekker, R.

doi:10.1109/memsys.2018.8346549

Cited by 5 publications

(5 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MEA and organ‐on‐a‐chip were embedded in porous plates by a thin‐film‐assisted molding process, and the biocompatibility of the porous plates and cells was proved by culturing human iPSC‐derived cardiomyocytes in the holes. [ 98 ]…”

Section: Functional Analysis and Simulation Methods Of Neuromuscular ...mentioning

confidence: 99%

“…The MEA and organ-on-a-chip were embedded in porous plates by a thin-filmassisted molding process, and the biocompatibility of the porous plates and cells was proved by culturing human iPSC-derived cardiomyocytes in the holes. [98] The MEA can provide spatiotemporally controlled electrical stimulation for electrophysiologically active cells in microchambers with many array electrodes. Rabieh et al applied the MEA technique to the electrophysiological detection of SKM by coating individual rat SKM tubes with micron-sized mushroom-like microelectrodes, which formed a high sealing resistance between them.…”

Section: Multielectrode Arraysmentioning

confidence: 99%

See 1 more Smart Citation

Advances in In Vitro Models of Neuromuscular Junction: Focusing on Organ‐on‐a‐Chip, Organoids, and Biohybrid Robotics

Leng

Zheng

et al. 2023

Advanced Materials

View full text Add to dashboard Cite

The neuromuscular junction (NMJ) is a peripheral synaptic connection between presynaptic motor neurons and postsynaptic skeletal muscle fibers that enables muscle contraction and voluntary motor movement. Many traumatic, neurodegenerative, and neuroimmunological diseases are classically believed to mainly affect either the neuronal or the muscle side of the NMJ, and treatment options are lacking. Recent advances in novel techniques have helped develop in vitro physiological and pathophysiological models of the NMJ as well as enable precise control and evaluation of its functions. This paper reviews the recent developments in in vitro NMJ models with 2D or 3D cultures, from organ‐on‐a‐chip and organoids to biohybrid robotics. Related derivative techniques are introduced for functional analysis of the NMJ, such as the patch‐clamp technique, microelectrode arrays, calcium imaging, and stimulus methods, particularly optogenetic‐mediated light stimulation, microelectrode‐mediated electrical stimulation, and biochemical stimulation. Finally, the applications of the in vitro NMJ models as disease models or for drug screening related to suitable neuromuscular diseases are summarized and their future development trends and challenges are discussed.

show abstract

Section: Functional Analysis and Simulation Methods Of Neuromuscular ...mentioning

confidence: 99%

Section: Multielectrode Arraysmentioning

confidence: 99%

Advances in In Vitro Models of Neuromuscular Junction: Focusing on Organ‐on‐a‐Chip, Organoids, and Biohybrid Robotics

Leng

Zheng

et al. 2023

Advanced Materials

View full text Add to dashboard Cite

show abstract

“…Additionally, endothelial and epithelial TCs have been fabricated with micro/nanoporous membranes coupled with electrodes to allow for non-destructive TEER measurements [ 186 , 187 ]. Micromachined electrodes have also been used to measure cellular electrical resistance and impedance, which provide information regarding cell size and intracellular complexity [ 152 , 188 ]. Sensors have been developed to measure local oxygen, pH, and levels of signaling molecules, such as nitric oxide [ 84 , 189 , 190 ].…”

Section: Challenges Associated With Design and Construction Of Micmentioning

confidence: 99%

Tissue Chips and Microphysiological Systems for Disease Modeling and Drug Testing

et al. 2021

View full text Add to dashboard Cite

Tissue chips (TCs) and microphysiological systems (MPSs) that incorporate human cells are novel platforms to model disease and screen drugs and provide an alternative to traditional animal studies. This review highlights the basic definitions of TCs and MPSs, examines four major organs/tissues, identifies critical parameters for organization and function (tissue organization, blood flow, and physical stresses), reviews current microfluidic approaches to recreate tissues, and discusses current shortcomings and future directions for the development and application of these technologies. The organs emphasized are those involved in the metabolism or excretion of drugs (hepatic and renal systems) and organs sensitive to drug toxicity (cardiovascular system). This article examines the microfluidic/microfabrication approaches for each organ individually and identifies specific examples of TCs. This review will provide an excellent starting point for understanding, designing, and constructing novel TCs for possible integration within MPS.

show abstract

“…The authors showed how state-of-the-art silicon-based micro-fabrication techniques allows generation of a versatile yet easily scalable toolkit for organ-on-a-chip devices and show very rudimentary proof-of-principle studies on immune cell chemotaxis, field potential/impedance recordings of human induced pluripotent stem cell (hiPSCs) and more physiological phenotype of hiPSC-derived cardiomyocytes on patterned membranes compared to flat membrane. In a more recent effort, the same authors presented protocols on how to mold and assemble these modular “cytostrech” modules in a 2 × 2 array suitable for multi-organ- or body-on-a-chip applications [71].…”

Section: Bio Multi Organ and Human-on-a-chip Systemsmentioning

confidence: 99%

Latest Trends in Biosensing for Microphysiological Organs-on-a-Chip and Body-on-a-Chip Systems

et al. 2019

View full text Add to dashboard Cite

Organs-on-chips are considered next generation in vitro tools capable of recreating in vivo like, physiological-relevant microenvironments needed to cultivate 3D tissue-engineered constructs (e.g., hydrogel-based organoids and spheroids) as well as tissue barriers. These microphysiological systems are ideally suited to (a) reduce animal testing by generating human organ models, (b) facilitate drug development and (c) perform personalized medicine by integrating patient-derived cells and patient-derived induced pluripotent stem cells (iPSCs) into microfluidic devices. An important aspect of any diagnostic device and cell analysis platform, however, is the integration and application of a variety of sensing strategies to provide reliable, high-content information on the health status of the in vitro model of choice. To overcome the analytical limitations of organs-on-a-chip systems a variety of biosensors have been integrated to provide continuous data on organ-specific reactions and dynamic tissue responses. Here, we review the latest trends in biosensors fit for monitoring human physiology in organs-on-a-chip systems including optical and electrochemical biosensors.

show abstract

A multiwell plate Organ-on-Chip (OOC) device for in-vitro cell culture stimulation and monitoring

Cited by 5 publications

References 8 publications

Advances in In Vitro Models of Neuromuscular Junction: Focusing on Organ‐on‐a‐Chip, Organoids, and Biohybrid Robotics

Advances in In Vitro Models of Neuromuscular Junction: Focusing on Organ‐on‐a‐Chip, Organoids, and Biohybrid Robotics

Tissue Chips and Microphysiological Systems for Disease Modeling and Drug Testing

Latest Trends in Biosensing for Microphysiological Organs-on-a-Chip and Body-on-a-Chip Systems

Contact Info

Product

Resources

About