A Multistage Volumetric Bar Chart Chip for Visualized Quantification of DNA

Abstract

Nucleic acid detection is critical in disease diagnosis as well as in the environmental assays of harmful bacteria or viruses and forensic applications. Current methods for visualized quantification of DNA require costly and sophisticated instruments. Here, we report a multistage propelled volumetric bar chart chip (MV-Chip) for multiplexing and quantitative detection of DNA. Owing to its ‘rocket-like’ propelling reaction, the pre-deposited platinum films could perform cascade amplification and detect as low as 20 pM DNA targets after three stages platinum catalyzed propulsion. The resulting ink bar charts can be directly read out by the naked eye and the signal shows little interference from serum. Single-nucleotide polymorphism and multiplex DNA detection were carried out to demonstrate this powerful application.

Microfluidics technology: future prospects for molecular diagnostics

“…The limit of detection (LOD) of this system was ~10 nM ssDNA from throat swab samples, which was 4 orders of magnitude below clinical viral titers and >2 orders of magnitude lower than rapid tests for swine influenza viruses. Song et al 58 developed a multistage volumetric bar chart chip (MV-Chip) for multiplex quantitative DNA detection. The microfluidic chip was fabricated using two glass slides (75×50×1.0 mm).…”

A versatile quantitation platform based on platinum nanoparticles incorporated volumetric bar-chart chip for highly sensitive assays

“…The devices were prepared as our reported methods (Song et al, 2013;Song et al, 2012). The standard photolithography and wet etching processes were used to fabricate the 3-plex V-Chip (3V-Chip) device.…”

“…For preparation of DNA-antibody complexes, 30 μL 0.1 M sodium borate buffer (pH 9.2) was firstly added to 60 μL of 1 mM DNA initiator (Ai) in double-distilled water (ddH 2 O) (Song et al, 2013). Then, 1 mL of 20 mg/mL p-phenylene diisothiocyanate (PDITC) in DMF was added and the solution was mixed and kept shaking for 2 h at room temperature in the dark.…”

“…In our original design, catalase was employed as the probe to transfer the biorecognition events into visualized and quantitative ink-bar chart results (Song et al, 2013(Song et al, , 2012. Subsequently, platinum nanoparticle (PtNPs) was reported as the perfect substitute for catalase that can react with hydrogen peroxide to produce oxygen gas (Li et al, 2016;Song et al, 2014b).…”

Polymeric-Based In Vitro Diagnostic Devices

“…Sensitivity can be enhanced by introducing new signal amplification approaches into ELISA or possessing more binding sites for enzymes conjugate. The plasmonic effect of AuNPs has been adopted and incorporated into an ELISA for colorimetric detection of proteins [307]. In addition, electrostatic interactions between green fluorescent proteins (GFP) and AuNPs have been used for protein detection in undiluted serum [293].…”