A multispectral microscope forin vivooximetry of rat dorsal spinal cord vasculature

Abstract

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Low-cost, multispectral imaging mini-microscope for longitudinal oximetry in small animals.

“…Universal port that holds a GRIN lens (GRINtech), fiber-array structured illumination adapted to Raspberry Pi camera, those were used to build a low-cost system for imaging [3]. In addition, the system is capable of directing the illumination into an annular illumination pattern, allowing a single pass illumination [5], i.e. reducing the complexity of the oxygenation measurement in deep tissue and blood vessels or for a better understanding of axonal remyelination, multiple sclerosis research, immune cells in lymph nodes and towards a cure for rheumatoid arthritis.…”

“…Recent evidence has implicated mitochondrial dysfunction, and hypoxia, in the aetiology and pathology of multiple sclerosis (MS) and experimental autoimmune encephalomyelitis, an animal model of MS [4,5]. The role of these pathological changes in neurological dysfunction, lesion formation, and disease progression remains unclear.…”

Multispectral oximetry of murine tendon microvasculature with inflammation

“…The data would then be applied to the model in a series of iterative nonlinear least-squares fits in order to extract unknown parameters, including SO 2 . Based on the modified Lambert-Beer law, previous work by van der Putten et al [18] and Smith et al [19], the optical transmission of a blood vessel can be written as:…”

Spectroscopic oximetry in the eye: a review

“…multispectral algorithms are T can provide quantitative oximetry in blood vessels where OS levels have not yet been measured by other means (e.g. in the spinal cord) [38] or in blood vessels that may be expected to be very different from physiological norms (e.g. studying angiogenesis in tumour development) [39,40].…”

“…For example, multispectral imaging oximetry has been employed to investigate diverse applications, including murine brain vascular oximetry, [55] diabetic foot-ulcer development, [56] bowel laproscopy, [57] skin blood flow, [58] the study of skin damage due to beta radiation exposure, [59] labial, periodontal, and sublingual microvasculature, [60] cancer tumour development in murine models, [39,40] and oximetry of the rat spinal cord dorsal vein for studying the development of multiple sclerosis disease models. [38]…”