A multiplex RT-PCR assay for the detection of fish picornaviruses

Mor, Sunil K.; Phelps, Nicholas B. D.; Barbknecht, Marisa; Hoffman, Michael A.; Goyal, Sagar M.

doi:10.1016/j.jviromet.2015.04.025

Cited by 3 publications

(1 citation statement)

References 6 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A semi‐nested RT‐PCR protocol was used to test for the presence of SVCV (USFWS and AFS‐FHS ), whereas the RT‐PCR assays of Mor et al. () and Hutchings () were utilized to test for the presence of FHMPV and WSBV, respectively (Table ).…”

Section: Methodsmentioning

confidence: 99%

Retail Baitfish in Michigan Harbor Serious Fish Viral Pathogens

et al. 2018

View full text Add to dashboard Cite

Indigenous small cyprinid fish species play an important role in Great Lakes ecosystems and also comprise the backbone of a multimillion-dollar baitfish industry. Due to their widespread use in sport fisheries of the Laurentian Great Lakes, there are increasing concerns that baitfish may introduce or disseminate fish pathogens. In this study, we evaluated whether baitfish purchased from 78 randomly selected retail bait dealers in Michigan harbored fish viruses. Between September 2015 and June 2016, 5,400 baitfish divided into 90 lots of 60 fish were purchased. Fish were tested for the presence of viral hemorrhagic septicemia virus (VHSV), spring viremia of carp virus (SVCV), golden shiner reovirus (GSRV), fathead minnow nidovirus (FHMNV), fathead minnow picornavirus (FHMPV), and white sucker bunyavirus (WSBV). Using the epithelioma papulosum cyprini cell line and molecular confirmation, we demonstrated the presence of viruses in 18 of the 90 fish lots (20.0%) analyzed. The most prevalent virus was FHMNV, being detected in 6 of 30 lots of Fathead Minnow Pimephales promelas and 3 of 42 lots of Emerald Shiners Notropis atherinoides. We also confirmed GSRV in two fish species: the Golden Shiner Notemigonus crysoleucas (5 of 11 lots) and Fathead Minnow (3 of 30 lots). Two VHSV (genotype IVb) isolates were recovered from a single lot of Emerald Shiners. No SVCV, FHMPV, or WSBV was detected in any of the fish examined. Some of the infected fish exhibited clinical signs and histopathological alterations. This study demonstrates that live baitfish are a potential vector for the spread of viral pathogens and underscores the importance of fish health certifications for the Great Lakes baitfish industry.

show abstract

Section: Methodsmentioning

confidence: 99%