A multiplex RNA quantification method to determine the absolute amounts of mRNA without reverse transcription

DNA Photo-cross-linking Using Pyranocarbazole and Visible Light

“…We have previously studied 3cyanovinylcarbazole nucleoside ( CNV K), which exhibits ultrafast cross-linking to pyrimidine bases in complementary DNA or RNA strands within 1 s under 366 nm UV-A irradiation. 7 This reversible photo-cross-linking is highly useful for antisense strategies, 8 the detection of a DNA or RNA strand in a sequence-specific manner, 9 and the construction and stabilization of nanostructured DNA. 10 Therefore, this ultrafast reversible photo-cross-linking has great potential for biological and nanotechnological applications.…”