A multiplex PCR method to detect 14 Escherichia coli serogroups associated with urinary tract infections

Li, Dan; Liu, Bin; Chen, Min; Guo, Dan; Guo, Xi; Liu, Fenxia; Feng, Lu; Wang, Lei

doi:10.1016/j.mimet.2010.04.008

Cited by 89 publications

(67 citation statements)

References 51 publications

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“…Therefore the present study was carried out on uncomplicated cases. Serogrouping of each ESBL isolate showed results similar to other studies [2].…”

Section: Discussionsupporting

confidence: 87%

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Clinical Significance of ESBL-Producing Escherichia coli Isolated from Urinary Tract Infections in Sylhet Bangladesh

Mahmood¹

2017

JBMOA

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The prevalence of extended-spectrum β-lactamases (ESBL) producing E. coli in the town of Sylhet was assessed over 12 months period. One hundred patients who had symptomatic urinary tract infections were selected for this study and urine samples were collected from the private laboratories of the community. These isolates were further confirmed as ESBL-producing E. coli by phenotypic methods were identified and selected. Patients having urinary tract infection showed 72% were female and the rest 28% were male. The investigations were carried out on female population only. The patient's age ranged from 4 years to 60 years. The highest age incidence of UTI patient was 21-30 years (28%) followed by 31-40 years (20%). Patients in the age range 41-50 years showed15% and patients above 50 years 19%. The antibiotic susceptibility test for non-ESBL showed 100% sensitivity to imipenem and meropenem. There was multidrug resistance to amoxicillin-clavulanic acid 30% ceftazidime 40%, ceftriaxone 35%, and ciprofloxacin 60%. The ESBL group were also 100% sensitive to carbapenems and the rest of resistance by percentage were amoxicillin-clavulanic acid -80%, ceftazidime-55%, ceftriaxone-80 %, ciprofloxacin 80%. The ESBL and non ESBL producing strains were separated for plasmid profile analysis. The plasmid profile showed 60% of isolates exhibit high molecular weight plasmids (>140MDa) in ESBL groups and similar results were seen in Non ESBL groups (64%). These findings suggests that both ESBL and non ESBL producing isolates harbour large size plasmids, despite the fact that ESBL-producing strains cause wide range of multi-drug resistance in the community.

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“…Therefore the present study was carried out on uncomplicated cases. Serogrouping of each ESBL isolate showed results similar to other studies [2].…”

Section: Discussionsupporting

confidence: 87%

“…Infections of the urinary tract are the second most common type of infection in the human infections and are mainly caused by uropathogenic E. coli. These bacteria are responsible for between 70% to 90 %, of community-acquired UTI [2]. They contribute largely (38-40%) to nosocomial infections [3].…”

Section: Introductionmentioning

confidence: 99%

Clinical Significance of ESBL-Producing Escherichia coli Isolated from Urinary Tract Infections in Sylhet Bangladesh

Mahmood¹

2017

JBMOA

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“…For the ESBL-producing UT strains, we determined the O serogroups by O-genotyping PCR (41,42). The H serogroup was determined by sequencing of the fliC gene (43) (see Table S2 in the supplemental material).…”

Section: Subjects and Strainsmentioning

confidence: 99%

Phylogenetic Analysis of Enteroaggregative Escherichia coli (EAEC) Isolates from Japan Reveals Emergence of CTX-M-14-Producing EAEC O25:H4 Clones Related to Sequence Type 131

et al. 2016

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f Enteroaggregative Escherichia coli (EAEC) causes acute or persistent diarrhea. The aggR gene is widely used as a marker for typical EAEC. The heterogeneity of EAEC is well known; however, there are few reports on the phylogenetic relationships of EAEC. Recently, CTX-M extended-spectrum ␤-lactamase (ESBL)-producing EAEC strains have been reported worldwide. To characterize EAEC strains in Japan, we investigated the population structure of EAEC. A total of 167 aggR-positive strains isolated from stool specimens from diarrheal patients in Kagoshima (139 strains) and Osaka (28 strains), Japan, between 1992 and 2010 were examined for the prevalence of EAEC virulence markers, the bla CTX-M gene, and the capacity to form biofilms. Multilocus sequence typing was also conducted. EAEC strains were widely distributed across four major E. coli phylogroups. Strains of O111: H21/clonal group 40 (CG40) (30 strains), O126:H27/CG200 (13 strains), and O86a:H27/CG3570 (11 strains) in phylogroup B1 are the historical EAEC clones in Japan, and they exhibited strong biofilm formation. Twenty-nine strains of EAEC O25:H4/ CG131 were identified in phylogroup B2, 79% of which produced CTX-M-14. This clone has emerged since 2003. The clone harbored plasmid-encoded EAEC virulence genes but not chromosomal virulence genes and had lower biofilm-forming capacity than historical EAEC strains. This clone most likely emerged from a pandemic uropathogenic O25:H4/sequence type 131 clone by acquiring an EAEC virulence plasmid from canonical EAEC. Surveillance of the horizontal transfer of both virulence and ESBL genes among E. coli strains is important for preventing a worldwide increase in antimicrobial drug resistance. Enteroaggregative Escherichia coli (EAEC) causes acute or persistent diarrhea in children and adults, in both developing (1) and industrialized (2, 3) countries. Several outbreaks of gastroenteritis linked to EAEC have been reported (4, 5). EAEC is also the second most common cause of travelers' diarrhea (6). EAEC is defined by its characteristic aggregative adherence (AA) to HEp-2 cells in culture (7). Although detection of the AA phenotype in the HEp-2 cell adherence assay is still the gold standard for EAEC identification, the transcriptional regulator AggR, which controls the expression of multiple EAEC virulence genes, is also used as a typical EAEC marker for PCR detection (8).The pathogenesis of EAEC infection involves the adherence of the bacterium to the intestinal mucosa, followed by the secretion of one or more enterotoxins and/or cytotoxins that induce mucosal inflammation (9). EAEC adherence to the mucosa is characterized by the formation of a thick biofilm (10), which may promote persistent infection. The majority of EAEC strains carry a large (ϳ100-kb) plasmid, which encodes most putative EAEC virulence factors, such as AggR, aggregative adherence fimbriae (AAF), the dispersin protein Aap (11), its transporter Aat (12), the plasmid-encoded toxin Pet (13), enteroaggregative heat-stable toxin 1 (EAST-1) (14), and the...

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“…There are 14 serogroups of UPEC that are most frequently found in UTIs and 75 % of UTIs are caused by serogroups O4, O6, O14, O22, O75 and O83 (Li et al, 2010). O2, O4, O6 and O75 are the most common serogroups causing UTIs worldwide (George & Manges, 2010).…”

Section: Introductionmentioning

confidence: 99%

Recurrent urinary tract infection due to co‐infection with extended spectrum β‐lactamase‐producer uropathogenic Escherichia coli and enteroaggregative E. coli

et al. 2014

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Introduction: Urinary tract infections (UTIs) are the most common bacterial infections in women of all ages. The emergence of resistance to newer classes of antibiotics, including third generation cephalosporins, has limited the drug choices for treatment of UTI. We report here a recurrent UTI due to co-infection with multiple-antibiotic-resistant (MAR) Escherichia coli pathotypes.Case presentation: A 73-year-old woman with diabetes mellitus type 2 presented with fever, nausea, vomiting, burning sensation, painful and frequent urination. She was diagnosed with recurrent UTI (RUTI) due to co-infection with pansensitive enteroaggregative E. coli (EAEC) and MAR extended spectrum b-lactamase (ESBL)-producing uropathogenic E. coli (UPEC) and treated with azithromycin and levofloxacin (each for 10 days). Unfortunately, she did not respond. Plasmid profile analysis showed that the MAR E. coli strain harboured multiple plasmids including ,38 and ,80 MDa plasmids. PCR for detection of b-lactamase genes showed that the isolate was negative for blaTEM, blaSHV, blaOXA and blaCTX-M. To the best of our knowledge, this is the first report of co-infection with an EAEC and MAR ESBL-producer UPEC in Bangladesh. Conclusion:We recommend that all pathotypes of E. coli as well as the other more common uropathogens should be considered in the diagnosis of RUTI and multiple antibiotics should be prescribed only in severe conditions.

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A multiplex PCR method to detect 14 Escherichia coli serogroups associated with urinary tract infections

Cited by 89 publications

References 51 publications

Clinical Significance of ESBL-Producing Escherichia coli Isolated from Urinary Tract Infections in Sylhet Bangladesh

Clinical Significance of ESBL-Producing Escherichia coli Isolated from Urinary Tract Infections in Sylhet Bangladesh

Phylogenetic Analysis of Enteroaggregative Escherichia coli (EAEC) Isolates from Japan Reveals Emergence of CTX-M-14-Producing EAEC O25:H4 Clones Related to Sequence Type 131

Recurrent urinary tract infection due to co‐infection with extended spectrum β‐lactamase‐producer uropathogenic Escherichia coli and enteroaggregative E. coli

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