A Multiplex Immunoassay for Human Adipokine Profiling

Bateman, Scott; Jager, Wilco de; Dijk, Marijke van; Meerding, Jenny; Zelissen, Pierre; Adan, Roger A.H.; Prakken, Berent J.; Kalkhoven, Eric

doi:10.1373/clinchem.2010.146118

Cited by 46 publications

(50 citation statements)

References 29 publications

(44 reference statements)

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“…For a broader investigation of biomarkers, some working groups applied multiplex cytokine measurements in the blood samples of MetS patients and reported a range of cytokine concentrations comparable to those measured in our study (Schipper et al, 2010;Bremer et al, 2011;Sharabiani et al, 2011;Surendar et al, 2011;Tisato et al, 2013). We identified IL-6 and IL-10 to be significantly elevated in MetS patients.…”

Section: Discussionsupporting

confidence: 74%

“…These data highlight a specific low-grade inflammation in obesity/MetS, with the majority of cytokine levels tested not being increased in blood. However, the reports show some variation related to gender (Saltevo et al, 2008), ethnicity (Morimoto et al, 2014), sample size (Schipper et al, 2010;Phillips & Perry, 2013), clinical heterogeneity of MetS (Phillips & Perry, 2013), and different definitions of MetS (Surendar et al, 2011;Tisato et al, 2013). Specifically, increased waist circumference is mandatory in the IDF definition, while being just one facultative parameter in the NCEP definition (Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults, 2001;Alberti et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

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Metabolic syndrome and the seminal cytokine network in morbidly obese males

et al. 2016

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SUMMARYGiven the increasing prevalence of metabolic syndrome (MetS) in males of reproductive age, the objective of this prospective casecontrolled study was to investigate the impact of subacute systemic inflammation associated with MetS on seminal cytokines and standard sperm parameters in comparison with healthy men. Between 2011 and 2014, we recruited 27 patients with MetS out of 41 obese patients screened from an internal outpatient clinic. Twenty-seven age-matched healthy controls were enrolled from 54 men requesting vasectomy in a urological outpatient clinic. A multiplex analysis was performed to quantify simultaneously the level of 30 cytokines (Eotaxin, FGF, Fraktalkine, GCSF, GMCSF, Granzyme A, IFN-c, IL-1a, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IP-10, I-TAC, MCP-1, MIG, MIP-1a, MIP-1b, RANTES, TNF-a, and VEGF) in each 50 lL of blood and seminal plasma during the andrological work-up. Semen analysis was performed according to the WHO (Global status report on noncommunicable diseases, 2010) recommendations, including standard sperm parameters as well as peroxidase-positive leukocytes and polymorphonuclear elastase. Blood levels of C-reactive protein, interleukins 6 and 10 were elevated in MetS (p > 0.001). Two-way hierarchical cluster analysis showed characteristic cytokine networks in semen greatly differing from those in blood, but not between MetS and controls. No deterioration of semen analysis was evident in men diagnosed with MetS. Our results suggest that there is no transmission of the systemic inflammation associated with MetS into semen based on cytokine profiles and that MetS does not impair standard semen parameters to a clinically significant extent.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Metabolic syndrome and the seminal cytokine network in morbidly obese males

et al. 2016

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“…The analysis protocol as well as the characteristics of the assay have been published previously. 14 Determination of adipocyte size, macrophage infiltration and capillary density in adipose tissue samples…”

Section: Determination Of Adipokine and Cytokine Concentrations In Blmentioning

confidence: 99%

Adipose tissue quantity and composition contribute to adipokine concentrations in the subclavian vein and the inferior mesenteric vein

et al. 2011

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BACKGROUND: Adipose tissue dysfunction is associated with inflammation, type 2 diabetes mellitus and vascular diseases. Visceral adipose tissue (VAT)-derived adipokines, which are released in the portal circulation may influence liver metabolism. OBJECTIVES: (1) To estimate the contribution of VAT and subcutaneous adipose tissue (SAT) on adipokine levels by measuring differences in adipokine concentrations between the portal draining inferior mesenteric vein and the subclavian vein.(2) To determine the relation of both VAT and SAT quantity and composition to mesenteric and systemic concentrations of adipokines. DESIGN: Cross-sectional cohort study. SUBJECTS: A total of 32 patients undergoing abdominal aortic surgery. MEASUREMENTS: A panel of 18 adipokines was measured in perioperatively obtained blood samples from the subclavian vein and the inferior mesenteric vein. Adipocyte size, macrophage infiltration and capillary density were measured in subcutaneous and mesenteric adipose tissue biopsies; SAT and VAT areas were measured on computed tomography images. RESULTS: Serum interferon-g-inducible protein 10 (IP-10) and hepatocyte growth factor (HGF) concentrations were significantly higher in the inferior mesenteric vein vs the subclavian vein. SAT area (b --18; 95% confidence interval (CI) À35 to À2), subcutaneous adipocyte size (b --488; 95% CI À938 to À38) and SAT macrophages quantity (b --1439; 95% CI À2387 to À491) were negatively associated with adiponectin levels in the systemic circulation. SAT area was related to systemic concentrations of leptin. Mesenteric adiponectin concentrations were related to VAT area (b --20; 95% CI À35 to À5) and visceral adipocyte size (b À1076; 95% CI À1624 to À527). VAT area, adipocyte size and capillary density were related to systemic adiponectin concentrations. CONCLUSION: SAT and VAT quantities as well as morphologic characteristics of both adipose tissue depots are related to systemic and mesenteric adipokine concentrations. There were no differences in adipokine concentrations between the mesenteric and subclavian vein, except for higher IP-10 and HGF concentrations in the inferior mesenteric vein, indicating a possible contribution of VAT to IP-10 and HGF levels.

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“…The adiponectin secretion of SGBS adipocytes transduced with empty vector and CD1d shRNA constructs was measured making use of a recently developed and validated multiplex immunoassay (72).…”

mentioning

confidence: 99%

“…Finally, the suspended iNKT cell fraction was pelleted for intracellular cytokine staining, and the supernatant was stored at -80°C until analysis of cytokine levels. Cytokine levels were measured with a cytokine multiplex immunoassay, as described recently (72).…”

mentioning

confidence: 99%

Natural killer T cells in adipose tissue prevent insulin resistance

Bateman¹,

Rakhshandehroo²,

Graaf³

et al. 2012

J. Clin. Invest.

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185

207

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Lipid overload and adipocyte dysfunction are key to the development of insulin resistance and can be induced by a high-fat diet. CD1d-restricted invariant natural killer T (iNKT) cells have been proposed as mediators between lipid overload and insulin resistance, but recent studies found decreased iNKT cell numbers and marginal effects of iNKT cell depletion on insulin resistance under high-fat diet conditions. Here, we focused on the role of iNKT cells under normal conditions. We showed that iNKT cell-deficient mice on a low-fat diet, considered a normal diet for mice, displayed a distinctive insulin resistance phenotype without overt adipose tissue inflammation. Insulin resistance was characterized by adipocyte dysfunction, including adipocyte hypertrophy, increased leptin, and decreased adiponectin levels. The lack of liver abnormalities in CD1d-null mice together with the enrichment of CD1d-restricted iNKT cells in both mouse and human adipose tissue indicated a specific role for adipose tissue-resident iNKT cells in the development of insulin resistance. Strikingly, iNKT cell function was directly modulated by adipocytes, which acted as lipid antigen-presenting cells in a CD1d-mediated fashion. Based on these findings, we propose that, especially under low-fat diet conditions, adipose tissue-resident iNKT cells maintain healthy adipose tissue through direct interplay with adipocytes and prevent insulin resistance.

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A Multiplex Immunoassay for Human Adipokine Profiling

Cited by 46 publications

References 29 publications

Metabolic syndrome and the seminal cytokine network in morbidly obese males

Metabolic syndrome and the seminal cytokine network in morbidly obese males

Adipose tissue quantity and composition contribute to adipokine concentrations in the subclavian vein and the inferior mesenteric vein

Natural killer T cells in adipose tissue prevent insulin resistance

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