A multiple resistance locus on chromosome arm 3BS in wheat confers resistance to stem rust (Sr2), leaf rust (Lr27) and powdery mildew

Mago, Rohit; Tabe, Linda; McIntosh, R. A.; Pretorius, Z. A.; Kota, Raja; Paux, Etienne; Wicker, Thomas; Breen, James; Lagudah, Evans; Ellis, Jeff; Spielmeyer, Wolfgang

doi:10.1007/s00122-011-1611-y

Cited by 118 publications

(77 citation statements)

References 25 publications

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“…To date, more than five APR genes have been identified and validated by several studies (Sr2/Yr30/Lr27/Pbc1, Sr55/Lr67/Yr46/Pm46/Ltn3, Sr57/Lr34/Yr18/Pm38/ Ltn1, Sr58/Lr46/Yr29/Pm39/Ltn2 and Lr68) and confer resistance not only to stem rust but also to other diseases such as leaf rust, yellow rust and powdery mildew of wheat (William et al 2006;Lillemo et al 2008;Krattinger et al 2009;Herrera-Foessel et al 2011;Mago et al 2011a;Herrera-Foessel et al 2014;Kolmer et al 2015). Of the reported genes, our results were in agreement with that of Bajgain et al (2016) that Gem, Kudu, Paka and Romany all carry the Sr2 gene.…”

Section: Discussionmentioning

confidence: 99%

“…There is evidence that most APR genes are effective across a number of pathogens, e.g. Sr2/ Yr30/Lr27/Pbc1, Sr55/Lr67/Yr46/Pm46/Ltn3, Sr57/Lr34/Yr18/Pm38/Ltn1 and Sr58/ Lr46/Yr29/Pm39/Ltn2 (William et al 2006;Lillemo et al 2008;Krattinger et al 2009;Mago et al 2011a;Herrera-Foessel et al 2014;Kolmer et al 2015). Several Kenyan cultivars have been shown to be good sources of APR genes controlling stem rust (Knott 1968;Njau et al 2009Njau et al , 2010Onguso and Njau 2015;Bajgain et al 2016).…”

Section: Cereal Research Communicationsmentioning

confidence: 99%

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Adult plant resistance of selected Kenyan wheat cultivars to leaf rust and stem rust diseases

Figlan¹,

Baloyi²,

Hlongoane³

et al. 2017

Cereal Research Communications

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Phenotypic and genotypic evaluation of wheat genetic resources and development of segregating populations are pre-requisites for identifying rust resistance genes. The objectives of this study were to assess adult plant resistance (APR) of selected wheat genotypes to leaf rust and stem rust and to develop segregating populations for resistance breeding. Eight selected Kenyan cultivars with known resistance to stem rust, together with local checks were evaluated for leaf rust and stem rust resistance at seedling stage and also across several environments. Selected diagnostic markers were used to determine the presence of known genes. All eight cultivars were crossed with local checks using a bi-parental mating design. Seedling tests revealed that parents exhibited differential infection types against wheat rust races. Cultivars Paka and Popo consistently showed resistant infection types at seedling stage, while Gem, Romany, Pasa, Fahari, Kudu, Ngiri and Kariega varied for resistant and susceptible infection types depending on the pathogen race used. The control cultivars Morocco and McNair consistently showed susceptible infection types as expected. In the field, all cultivars except for Morocco showed moderate to high levels of resistance, indicating the presence of effective resistance genes. Using diagnostic markers, presence of Lr34 was confirmed in Gem, Fahari, Kudu, Ngiri and Kariega, while Sr2 was present in Gem, Romany, Paka and Kudu. Seedling resistance gene, Sr35, was only detected in cultivar Popo. Overall, the study developed 909 F 6:8 recombinant inbred lines (RILs) as part of the nested mating design and are useful genetic resources for further studies and for mapping wheat rust resistance genes.

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Section: Discussionmentioning

confidence: 99%

Section: Cereal Research Communicationsmentioning

confidence: 99%

Adult plant resistance of selected Kenyan wheat cultivars to leaf rust and stem rust diseases

Figlan¹,

Baloyi²,

Hlongoane³

et al. 2017

Cereal Research Communications

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“…tritici (Kota et al 2006), Qfhs.ndsu-3BS, a main QTL for resistance to Fusarium gramineum Schwabe (Liu et al 2005) and powdery mildew resistance, caused by Blumeria graminis f. sp. tritici (Mago et al 2011). Gene Sr2 was allocated in the same interval encompassed by markers gwm389 and gwm493 used in the present study (Spielmeyer et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of leaf rust resistance genes and associated markers in the durable resistant wheat cultivar Sinvalocho MA

et al. 2012

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In the cross of the durable leaf rust resistant wheat Sinvalocho MA and the susceptible line Gama6, four specific genes were identified: the seedling resistance gene Lr3, the adult plant resistance (APR) genes LrSV1 and LrSV2 coming from Sinvalocho MA, and the seedling resistance gene LrG6 coming from Gama6. Lr3 was previously mapped on 6BL in the same cross. LrSV1 was mapped on chromosome 2DS where resistance genes Lr22a and Lr22b have been reported. Results from rust reaction have shown that LrSV1 from Sinvalocho is not the same allele as Lr22b and an allelism test with Lr22a showed that they could be alleles or closely linked genes. LrSV1 was mapped in an 8.5-cM interval delimited by markers gwm296 distal and gwm261 proximal. Adult gene LrSV2 was mapped on chromosome 3BS, cosegregating with gwm533 in a 7.2-cM interval encompassed by markers gwm389 and gwm493, where other disease resistance genes are located, such as seedling gene Lr27 for leaf rust, Sr2 for stem rust, QTL Qfhs.ndsu-3BS for resistance to Fusarium gramineum and wheat powdery mildew resistance. The gene LrG6 was mapped on chromosome 2BL, with the closest marker gwm382 at 0.6 cM. Lines carrying LrSV1, LrSV2 and LrG6 tested under field natural infection conditions, showed low disease infection type and severity, suggesting that this kind of resistance can be explained by additive effects of APR and seedling resistance genes. The identification of new sources of resistance from South American land races and old varieties, supported by modern DNA technology, contributes to sustainability of agriculture through plant breeding.

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“…Pyramiding of race-specific resistance genes and the use of more durable, race nonspecific resistance genes are strategies to avoid rapid adaptation of pathogens in the field (Brunner et al 2010;Lagudah 2011;Mago et al 2011: Sun et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Recent emergence of the wheat Lr34 multi-pathogen resistance: insights from haplotype analysis in wheat, rice, sorghum and Aegilops tauschii

Krattinger

Jordan

Mace³

et al. 2012

Theor Appl Genet

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Spontaneous sequence changes and the selection of beneficial mutations are driving forces of gene diversification and key factors of evolution. In highly dynamic co-evolutionary processes such as plant-pathogen interactions, the plant's ability to rapidly adapt to newly emerging pathogens is paramount. The hexaploid wheat gene Lr34, which encodes an ATP-binding cassette (ABC) transporter, confers durable field resistance against four fungal diseases. Despite its extensive use in breeding and agriculture, no increase in virulence towards Lr34 has been described over the last century. The wheat genepool contains two predominant Lr34 alleles of which only one confers disease resistance. The two alleles, located on chromosome 7DS, differ by only two exon-polymorphisms. Putatively functional homoeologs and orthologs of Lr34 are found on the B-genome of wheat and in rice and sorghum, but not in maize, barley and Brachypodium. In this study we present a detailed haplotype analysis of homoeologous and orthologous Lr34 genes in genetically and geographically diverse selections of wheat, rice and sorghum accessions. We found that the resistant Lr34 haplotype is unique to the wheat D-genome and is not found in the B-genome of wheat or in rice and sorghum. Furthermore, we only found the susceptible Lr34 allele in a set of 252 Ae. tauschii genotypes, the progenitor of the wheat D-genome. These data provide compelling evidence that the Lr34 multi-pathogen resistance is the result of recent gene diversification occurring after the formation of hexaploid wheat about 8,000 years ago. AbstractSpontaneous sequence changes and the selection of beneficial mutations are driving forces of gene diversification and key factors of evolution. In highly dynamic coevolutionary processes such as plant-pathogen interactions, the plant's ability to rapidly adapt to newly emerging pathogens is paramount. The hexaploid wheat gene Lr34, which encodes an ATP-binding cassette (ABC) transporter, confers durable field resistance against four fungal diseases. Despite its extensive use in breeding and agriculture, no increase in virulence towards Lr34 has been described over the last century. The wheat genepool contains two predominant Lr34 alleles of which only one confers disease resistance. The two alleles, located on chromosome 7DS, differ by only two exon polymorphisms. Putatively functional homoeologs and orthologs of Lr34 are found on the B-genome of wheat and in rice and sorghum, but not in maize, barley and Brachypodium. In this study we present a detailed haplotype analysis of homoeologous and orthologous Lr34 genes in genetically and geographically diverse selections of wheat, rice and sorghum accessions. We found that the resistant Lr34 haplotype is unique to the wheat D-genome and is not found in the B-genome of wheat or in rice and sorghum. Furthermore, we only found the susceptible Lr34 allele in a set of 252 Ae. tauschii genotypes, the progenitor of the wheat D-genome. These data provide compelling evidence that the Lr34 mult...

show abstract

A multiple resistance locus on chromosome arm 3BS in wheat confers resistance to stem rust (Sr2), leaf rust (Lr27) and powdery mildew

Cited by 118 publications

References 25 publications

Adult plant resistance of selected Kenyan wheat cultivars to leaf rust and stem rust diseases

Adult plant resistance of selected Kenyan wheat cultivars to leaf rust and stem rust diseases

Genetic analysis of leaf rust resistance genes and associated markers in the durable resistant wheat cultivar Sinvalocho MA

Recent emergence of the wheat Lr34 multi-pathogen resistance: insights from haplotype analysis in wheat, rice, sorghum and Aegilops tauschii

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