A multiple detection method for distinguishing gene mutations based on melting curves of extended quenching probes

Wang, Jianping; Liu, Zipeng; Tengfei, Pan; Song, Zhang

doi:10.1016/j.heliyon.2022.e11856

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…As a proof of concept, this system showcased the multiplexed detection of EGFR gene mutations in plasma samples, with a detection limit of 1 copy/μL. As both the acoustic resonator and a-Si:H chip are CMOS-compatible, such chip-scale liquid handling and sensing modules largely facilitate developing various LOC systems for POC applications. , …”

Section: Introductionmentioning

confidence: 99%

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Heterogeneous Integration of Acoustic Microextraction with an Optoelectronic Sensor on Glass for Nucleic Acid Testing

Li,

Costantini

et al. 2024

Anal. Chem.

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Lab-on-a-chip systems (LOCs), characterized by their high sensitivity, low sample consumption, and portability, have significantly advanced the field of on-site testing. Despite the evolution of integrated LOCs from qualitative to quantitative analyses, on-chip full integration of sample preparation, purification, and multiplexed detection remains a challenge. Here, we propose a strategy for the heterogeneous integration of a set of complementary metal oxide semiconductor-compatible devices including acoustic resonator, thin-film resistors, and temperature/photosensors as a new type of LOC for nucleic acid testing (NAT). Programmed acoustic streaming-based particles and fluid manipulations largely simplify the nucleic acid extraction process including cell lysis, nucleic acid capture, and elution. The design of the acoustic microextraction module and extraction process was thoroughly studied. Benefitted by the microelectromechanical system approach, the conventional mechanical actions and complex flow control are avoided, which enables a compact hand-held NAT instrument without complicated peripherals. Validation experiments conducted on plasma-harboring mutations in the epidermal growth factor receptor (EGFR) gene confirmed the robustness of the system, achieving an impressive nucleic acid (NA) extraction efficiency of approximately 90% within 5 min and a limit of detection of the target NA in the plasma of 1 copy/μL.

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Section: Introductionmentioning

confidence: 99%

“…As both the acoustic resonator and a-Si:H chip are CMOS-compatible, such chip-scale liquid handling and sensing modules largely facilitate developing various LOC systems for POC applications. 25,26 ■ MATERIALS AND METHODS Reagents and Samples. Healthy plasma was acquired from Guosheng Zhongyuan Co., Ltd. (Tianjin, China).…”

Section: ■ Introductionmentioning

confidence: 99%

Heterogeneous Integration of Acoustic Microextraction with an Optoelectronic Sensor on Glass for Nucleic Acid Testing

Li,

Costantini

et al. 2024

Anal. Chem.

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Rapid Capture of DNA from Human Urine Using Chitosan-Coated Nylon Membrane and Its Application

Ren,

Sun,

et al. 2023

Natl. Acad. Sci. Lett.

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A multiple detection method for distinguishing gene mutations based on melting curves of extended quenching probes

Cited by 2 publications

References 37 publications

Heterogeneous Integration of Acoustic Microextraction with an Optoelectronic Sensor on Glass for Nucleic Acid Testing

Heterogeneous Integration of Acoustic Microextraction with an Optoelectronic Sensor on Glass for Nucleic Acid Testing

Rapid Capture of DNA from Human Urine Using Chitosan-Coated Nylon Membrane and Its Application

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