A multimegabase cluster of snRNA and tRNA genes on chromosome 1p36 harbours an adenovirus/SV40 hybrid virus integration site

Drift, P. van der; Chan, Alvin; Roy, Nilanjan; Laureys, Geneviève; Westerveld, A.; Speleman, Frank; Versteeg, Rogier

doi:10.1093/hmg/3.12.2131

Cited by 19 publications

(24 citation statements)

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“…Viral integration may destabilise telomeres and other chromosomal regions, making them susceptible to recombination [Rogler et al, 1985;Sandros and Stenman, 1990;Kao et al, 1993;van der Drift et al, 1994;Wilke et al, 1996;Jox et al, 1997;Livezey and Simon, 1997;Lopez-Borges et al, 1998]. The low frequency of nonclonal chromosome aberrations we observed in PHA-stimulated cells may therefore be related to the integrated virus.…”

Section: Discussionmentioning

confidence: 80%

Fine mapping of an apparently targeted latent human herpesvirus type 6 integration site in chromosome band 17p13.3

et al. 1999

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An unusually high level of latent HHV-6 infection has been documented in the peripheral blood and/or bone marrow cells of a small group of patients with predominantly malignant lymphoid disorders, and in at least one healthy individual. We have shown previously in peripheral blood mononuclear cells (PBMCs) of three patients, two with a history of lymphoma and one with multiple sclerosis, a specific target site for latent integration of the full-length HHV-6 viral genome on the distal short arm of chromosome 17, in band p13.3. Fluorescence in situ hybridization (FISH) procedures were used to map more precisely the location of the viral integration site in one of those patients, relative to two known oncogenes mapped previously, namely CRK, and the more telomeric ABR oncogene. It is shown that the HHV-6 integration site is located at least 1,000 kb telomeric of ABR, and is very likely to map close to or within the telomeric sequences of 17p. This finding is significant given that human telomeric-like repeats flank the terminal ends of the HHV-6 genome. Cytogenetic studies showed evidence of karyotype instability in the peripheral blood cells infected latently.

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Section: Discussionmentioning

confidence: 80%

Fine mapping of an apparently targeted latent human herpesvirus type 6 integration site in chromosome band 17p13.3

et al. 1999

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“…This probe contains the 5' flanking region of RNUI, which recognizes the same fragments on lp36 as the previously described RNUl probe (Van der Drift et al, 1994), but which has hardly any homology with pseudogenes on l q (Lund and Dahlberg, 1984). CpG island probes were isolated by double digestion of total yeast DNA containing YACs with the restriction enzymes BssHIT or Not1 and Hind111 and cloning of the fragments in vectors pNEB193 or pBS-SK.…”

Section: Materials a N D Methodsmentioning

confidence: 99%

Balanced translocation in a neuroblastoma patient disrupts a cluster of small nuclear RNA UI and tRNA genes in chromosomal band Ip36

Drift

Chan

Laureys

et al. 1995

Genes Chromosomes & Cancer

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Chromosomal band 1p36 probably harbours several neuroblastoma suppressor genes. A neuroblastoma patient has been described with a constitutional balanced translocation, t(1;17)(p36;q12-21). Cytogenetically, no loss of chromosomal material was visible. The 1p36 translocation breakpoint could therefore have inactivated one allele of a tumour suppressor gene, thus predisposing the patient to develop neuroblastoma. We localized this breakpoint by pulsed field gel electrophoresis, analysis of yeast artificial chromosomes, and fluorescence in situ hybridization. Here we report that the breakpoint is within a large cluster of small nuclear RNA U1 (RNU1) and some tRNA genes (TRE, TRN) on chromosomal band 1p36. The size of this cluster is over two megabases and it contains many other locally repeated sequences. Polyadenylated transcripts were identified for some of these sequences. In addition, the cluster is the target for integration of an adenovirus 5/SV40 hybrid virus. The translocation breakpoint maps distal of this viral integration site and proximal of marker PND.

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“…The human genome contains an estimated 1300 tRNA genes (Hatlen and Attardi 1971). Both individual genomic tRNA genes and tRNA gene clusters have been reported previously in humans (Zasloff and Santos 1980;Santos and Zasloff 1981;Roy et al 1982;Buckland et al 1983;Buckland 1989;van der Drift et al 1994;Buckland et al 1996). In none of these cases, however, is the complete sequence of a major human tRNA cluster fully delineated.…”

Section: Discussionmentioning

confidence: 94%

Construction of an ∼700-kb Transcript Map Around the Familial Mediterranean Fever Locus on Human Chromosome 16p13.3

Centola

Chen²,

Sood

et al. 1998

Genome Res.

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We used a combination of cDNA selection, exon amplification, and computational prediction from genomic sequence to isolate transcribed sequences from genomic DNA surrounding the familial Mediterranean fever (FMF) locus. Eighty-seven kb of genomic DNA around D16S3370, a marker showing a high degree of linkage disequilibrium with FMF, was sequenced to completion, and the sequence annotated. A transcript map reflecting the minimal number of genes encoded within the ∼700 kb of genomic DNA surrounding the FMF locus was assembled. This map consists of 27 genes with discreet messages detectable on Northerns, in addition to three olfactory-receptor genes, a cluster of 18 tRNA genes, and two putative transcriptional units that have typical intron-exon splice junctions yet do not detect messages on Northerns. Four of the transcripts are identical to genes described previously, seven have been independently identified by the French FMF Consortium, and the others are novel. Six related zinc-finger genes, a cluster of tRNAs, and three olfactory receptors account for the majority of transcribed sequences isolated from a 315-kb FMF central region (between D16S468/D16S3070 and cosmid 377A12). Interspersed among them are several genes that may be important in inflammation. This transcript map not only has permitted the identification of the FMF gene (MEFV), but also has provided us an opportunity to probe the structural and functional features of this region of chromosome 16.Saturating the human genome with mapped genes so that the structure and function of each can be determined is an ultimate goal of the human genome project (Collins and Galas 1993). In addition

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A multimegabase cluster of snRNA and tRNA genes on chromosome 1p36 harbours an adenovirus/SV40 hybrid virus integration site

Cited by 19 publications

References 0 publications

Fine mapping of an apparently targeted latent human herpesvirus type 6 integration site in chromosome band 17p13.3

Fine mapping of an apparently targeted latent human herpesvirus type 6 integration site in chromosome band 17p13.3

Balanced translocation in a neuroblastoma patient disrupts a cluster of small nuclear RNA UI and tRNA genes in chromosomal band Ip36

Construction of an ∼700-kb Transcript Map Around the Familial Mediterranean Fever Locus on Human Chromosome 16p13.3

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