A multifunctional, plug-and-play and low-cost microfluidic connector system based on electronics standard

Shuqing, Zhao; Chen, R.; Yu, Yang; Liu, J. Q.; Chen, X.; Qin, Sheng-Ying

doi:10.1039/c5ra22165j

Cited by 2 publications

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…23 This study developed a new automated drug screening chip based on the microfluidic oscillator, which had been described in our previous paper. 24 We then conducted a test that evaluates this wide-range dilution gradient chip.…”

Section: Introductionmentioning

confidence: 99%

A novel wide-range microfluidic dilution device for drug screening

et al. 2019

View full text Add to dashboard Cite

Microfluidic dilution chip is a crucial approach to perform gradient dilution of experimental samples in many biological investigations. In this study, we developed two serial wide-range dilution chips with dilution rates of 1:1 and 1:4 on the basis of the microfluidic oscillator by designing a series chamber, which was similar to a series circuit. The size of this chamber was adjusted and mixed with the neighboring air chamber to form dilution rates by oscillatory methods. We applied this microfluidic oscillator to estimate cellular kinetics and perform an acute oxidative stress test on Caenorhabditis elegans (C. elegans) in order to further validate their effectiveness. We estimated the kinetic parameters of β-galactosidase, the biocatalyst responsible for the hydrolysis of lactose, and found out that K m was 602 ± 73 μM and k cat was 72 ± 12/s. In addition, our result of the study on acute oxidative stress of C. elegans using this novel chip was consistent with the result using 96-well plates. Overall, we believe that this novel chip can be applied to enzymatic reaction kinetics to evaluate accurately drug screening in bio-nematode models such as C. elegans. In summary, we have provided a novel microfluidic dilution chip that can form a wide range of sample concentration gradients. Our chip may facilitate drug screening, drug toxicology, and environmental toxicology.

show abstract

Section: Introductionmentioning

confidence: 99%

A novel wide-range microfluidic dilution device for drug screening

et al. 2019

View full text Add to dashboard Cite

show abstract

3D printed high density, reversible, chip-to-chip microfluidic interconnects

2018

View full text Add to dashboard Cite

Our latest developments in miniaturizing 3D printed microfluidics [Gong et al., Lab Chip, 2016, 16, 2450; Gong et al., Lab Chip, 2017, 17, 2899] offer the opportunity to fabricate highly integrated chips that measure only a few mm on a side. For such small chips, an interconnection method is needed to provide the necessary world-to-chip reagent and pneumatic connections. In this paper, we introduce simple integrated microgaskets (SIMs) and controlled-compression integrated microgaskets (CCIMs) to connect a small device chip to a larger interface chip that implements world-to-chip connections. SIMs or CCIMs are directly 3D printed as part of the device chip, and therefore no additional materials or components are required to make the connection to the larger 3D printed interface chip. We demonstrate 121 chip-to-chip interconnections in an 11 × 11 array for both SIMs and CCIMs with an areal density of 53 interconnections per mm and show that they withstand fluid pressures of 50 psi. We further demonstrate their reusability by testing the devices 100 times without seal failure. Scaling experiments show that 20 × 20 interconnection arrays are feasible and that the CCIM areal density can be increased to 88 interconnections per mm. We then show the utility of spatially distributed discrete CCIMs by using an interconnection chip with 28 chip-to-world interconnects to test 45 3D printed valves in a 9 × 5 array. Each valve is only 300 μm in diameter (the smallest yet reported for 3D printed valves). Every row of 5 valves is tested to at least 10 000 actuations, with one row tested to 1 000 000 actuations. In all cases, there is no sign of valve failure, and the CCIM interconnections prove an effective means of using a single interface chip to test a series of valve array chips.

show abstract

A multifunctional, plug-and-play and low-cost microfluidic connector system based on electronics standard

Abstract: In this study, we utilized the electrical engineering standard of a 2.54 mm pin-header connector to create two standard microfluidic connectors and two accessories for different applications.

Cited by 2 publications

References 21 publications

A novel wide-range microfluidic dilution device for drug screening

A novel wide-range microfluidic dilution device for drug screening

3D printed high density, reversible, chip-to-chip microfluidic interconnects

Contact Info

Product

Resources

About