A multifunctional organosilica cross-linker for the bio-conjugation of gold nanorods

Lai, Sarah; Centi, Sonia; Borri, Claudio; Ratto, Fulvio; Cavigli, Lucia; Micheletti, Filippo; Kemper, Björn; Ketelhut, Steffi; Kozyreva, Tatiana; Gonnelli, Leonardo; Rossi, Francesca; Colagrande, Stefano; Pini, Roberto

doi:10.1016/j.colsurfb.2017.05.068

Cited by 20 publications

(38 citation statements)

References 74 publications

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“…The upper panel of Figure displays representative micrographs of cells treated for 24 hours with 50 ppm Au GNRs@CPP. As already reported elsewhere , the accumulation of GNRs did not modify the cellular morphology but for the occasional extrusion of pseudopodia and the occurrence of a dark particulate inside the perinuclear cytoplasm, probably consisting of endosomal vesicles enclosing a high density of gold. The lower panel of Figure displays the viability of cells treated with GNRs@CPP at different concentrations and incubation times.…”

Section: Resultssupporting

confidence: 82%

1064‐nm‐resonant gold nanorods for photoacoustic theranostics within permissible exposure limits

Cavigli

Centi

Borri

et al. 2019

Journal of Biophotonics

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Therapeutic and diagnostic methods based on photomechanical effects are attracting much current attention in contexts as oncology, cardiology and vascular surgery, for such applications as photoacoustic imaging or microsurgery. Their underlying mechanism is the generation of ultrasound or cavitation from the interaction of short optical pulses with endogenous dyes or targeted contrast agents. Among the latter, gold nanorods are outstanding candidates, but their use has mainly been reported for photoacoustic imaging and photothermal treatments. Conversely, much less is still known about their value as a precision tool for photomechanical manipulations, such as to impart local damage with high spatial resolution through the expansion and collapse of microbubbles. Here, we address the feasibility of gold nanorods exhibiting a distribution of surface plasmon resonances between about 900 to above 1100 nm as a contrast agent for photoacoustic theranostics. After testing their cytotoxicity and cellular uptake, we discuss their photostability and use to mediate cavitation and the photomechanical destruction of targeted cells. We find that the choice of a plasmonic band peaking around 1064 nm is key to enhance the translational potential of this approach. With respect to the standard alternative of 800 nm, at 1064 nm, relevant regulations on optical exposure are less restrictive and the photonic technology is more mature.

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Section: Resultssupporting

confidence: 82%

1064‐nm‐resonant gold nanorods for photoacoustic theranostics within permissible exposure limits

Cavigli

Centi

Borri

et al. 2019

Journal of Biophotonics

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“…As we explained above, Si and its compounds are among the best inorganic materials from the point of view of non‐disturbing character of interaction with biological systems. As an example, some previous studies used designed SERS probes based on silica (SiO 2 )‐coated plasmonic structures, including gold nanorods and nanostars , to improve the biocompatibility and efficiency of SERS probes. The inclusion of nonoxidized crystalline silicon (Si) fraction into composition of SERS probes is expected to further improve the biocompatibility of these probes, as Si is one of the essential materials in biological systems, which does not provoke any toxic effects even under massive injection in vitro or in vivo .…”

Section: Resultsmentioning

confidence: 99%

Bare laser‐synthesized Au‐based nanoparticles as nondisturbing surface‐enhanced Raman scattering probes for bacteria identification

Kögler

Ryabchikov

Uusitalo

et al. 2018

Journal of Biophotonics

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The ability of noble metal-based nanoparticles (NPs) (Au, Ag) to drastically enhance Raman scattering from molecules placed near metal surface, termed as surface-enhanced Raman scattering (SERS), is widely used for identification of trace amounts of biological materials in biomedical, food safety and security applications. However, conventional NPs synthesized by colloidal chemistry are typically contaminated by nonbiocompatible by-products (surfactants, anions), which can have negative impacts on many live objects under examination (cells, bacteria) and thus decrease the precision of bioidentification. In this article, we explore novel ultrapure laser-synthesized Au-based nanomaterials, including Au NPs and AuSi hybrid nanostructures, as mobile SERS probes in tasks of bacteria detection. We show that these Au-based nanomaterials can efficiently enhance Raman signals from model R6G molecules, while the enhancement factor depends on the content of Au in NP composition. Profiting from the observed enhancement and purity of laser-synthesized nanomaterials, we demonstrate successful identification of 2 types of bacteria (Listeria innocua and Escherichia coli). The obtained results promise less disturbing studies of biological systems based on good biocompatibility of contamination-free laser-synthesized nanomaterials.

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“…Instead, m-PEG-SH [alpha-methoxy-omega-mercapto-poly(ethyleneglycol)] and c-PEG-SH (alpha-carboxy-omega-mercaptopoly(ethylene glycol)), M w ≈ 5000 gmol −1 were acquired from Iris Biotech GmbH (Marktredwitz, Germany). CPPs were provided by Giotto Biotech Srl (Florence, Italy) [ 56 ]. All components for cell cultivation were obtained from Euroclone SpA (Pero, Italy).…”

Section: Methodsmentioning

confidence: 99%

“…These particles were imaged with a CM12 transmission electron microscope (TEM) from Philips (Amsterdam, the Netherlands), with a voltage of 100 kV and operating in standard conditions. MUTAB- and CPP-coated gold nanorods were prepared according to the prescriptions of refs [ 29 ] and [ 56 ], respectively. Instead, pLys-coated gold nanorods were realized by the same protocol that was implemented for the preparation of their CPP-coated counterpart, with the replacement of CPPs with the same dose of pLys.…”

Section: Methodsmentioning

confidence: 99%

“…However, quaternary ammonium compounds, such as MUTAB, are likely to cause cytotoxicity and so require caution [ 52 – 55 ]. More recently, we have reported on an alternative design that replaces these compounds with biocompatible arginine-rich cell penetrating peptides (CPPs) [ 56 ]. How natural CPPs really work remains a matter of debate but seems to relate to their abundance of cationic or lipophilic residues [ 57 – 60 ].…”

Section: Introductionmentioning

confidence: 99%

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Polylysine as a functional biopolymer to couple gold nanorods to tumor-tropic cells

et al. 2018

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BackgroundThe delivery of plasmonic particles, such as gold nanorods, to the tumor microenvironment has attracted much interest in biomedical optics for topical applications as the photoacoustic imaging and photothermal ablation of cancer. However, the systemic injection of free particles still crashes into a complexity of biological barriers, such as the reticuloendothelial system, that prevent their efficient biodistribution. In this context, the notion to exploit the inherent features of tumor-tropic cells for the creation of a Trojan horse is emerging as a plausible alternative.ResultsWe report on a convenient approach to load cationic gold nanorods into murine macrophages that exhibit chemotactic sensitivity to track gradients of inflammatory stimuli. In particular, we compare a new model of poly-l-lysine-coated particles against two alternatives of cationic moieties that we have presented elsewhere, i.e. a small quaternary ammonium compound and an arginine-rich cell-penetrating peptide. Murine macrophages that are exposed to poly-l-lysine-coated gold nanorods at a dosage of 400 µM Au for 24 h undertake efficient uptake, i.e. around 3 pg Au per cell, retain the majority of their cargo until 24 h post-treatment and maintain around 90% of their pristine viability, chemotactic and pro-inflammatory functions.ConclusionsWith respect to previous models of cationic coatings, poly-l-lysine is a competitive solution for the preparation of biological vehicles of gold nanorods, especially for applications that may require longer life span of the Trojan horse, say in the order of 24 h. This biopolymer combines the cost-effectiveness of small molecules and biocompatibility and efficiency of natural peptides and thus holds potential for translational developments.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0377-7) contains supplementary material, which is available to authorized users.

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A multifunctional organosilica cross-linker for the bio-conjugation of gold nanorods

Cited by 20 publications

References 74 publications

1064‐nm‐resonant gold nanorods for photoacoustic theranostics within permissible exposure limits

1064‐nm‐resonant gold nanorods for photoacoustic theranostics within permissible exposure limits

Bare laser‐synthesized Au‐based nanoparticles as nondisturbing surface‐enhanced Raman scattering probes for bacteria identification

Polylysine as a functional biopolymer to couple gold nanorods to tumor-tropic cells

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