A multifunctional neuromodulation platform utilizing Schwann cell-derived exosomes orchestrates bone microenvironment via immunomodulation, angiogenesis and osteogenesis

Hao, Zhichao; Ren, Lin; Zhang, Zhen; Yang, Zaiwu; Wu, Shujie; Liu, Gen; Cheng, Bin; Wu, Jun; Xia, Juan

doi:10.1016/j.bioactmat.2022.10.018

Cited by 27 publications

(29 citation statements)

References 65 publications

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“…In addition, Parfejevs et al found that SCs could activate the TGFβ signaling pathway to promote skin wound healing by increasing paracrine signaling [ 61 ]. Similarly, Hao et al found that the GelMA-loaded SC-exos could promote the osteogenic differentiation of BMSCs by activating the TGF-β/Smad signaling pathway [ 26 ]. Therefore, the activation of the TGFβ signaling pathway may be an important mechanism in SC-regulated tissue repair.…”

Section: Discussionmentioning

confidence: 99%

“…Su et al created a biomimetic periosteum loading SC-exos to enhance nerve repair for angiogenesis and bone regeneration [ 25 ]. Hao et al developed a multifunctional neuromodulation platform utilizing SC-exos that improved bone repair by regulating immunomodulation, angiogenesis, and osteogenesis [ 26 ]. These studies demonstrate that SC-exos are crucial paracrine signaling agents for SCs to regulate tissue repair and that the regulatory effects of SCs for bone repair in the microenvironment can be simulated via SC-exos.…”

Section: Introductionmentioning

confidence: 99%

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Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair

Wang

Zhang

et al. 2023

Bioactive Materials

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair

Wang

Zhang

et al. 2023

Bioactive Materials

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“…All animal experiments reported here were performed under guidelines evaluated and approved by the Animal Ethical Committee of Tianjin Medical University (NKYY-DWLL-2022-098). Thirty male SD rats (5–6 weeks old, 200–250 g) were used to establish two critical-sized (ϕ = 5 mm) skull defects besides the sagittal suture. , All rats were randomly divided into five groups: (1) control group, (2) GM microspheres filled group, (3) GMH microspheres filled group, (4) GMC microspheres filled group, and (5) GMHC microspheres filled group. The sterilized microspheres (∼10 mg) were dispersed in saline and injected into the defects, followed by suturing the overlying tissue.…”

Section: Materials and Methodsmentioning

confidence: 99%

Injectable, High Specific Surface Area Cryogel Microscaffolds Integrated with Osteoinductive Bioceramic Fibers for Enhanced Bone Regeneration

Wang

Yuan

Pang

et al. 2023

ACS Appl. Mater. Interfaces

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Organic−inorganic composites with high specific surface area and osteoinductivity provide a suitable microenvironment for cell ingrowth and effective ossification, which could greatly promote bone regeneration. Here, we report gelatin methacryloyl (GelMA) cryogel microspheres that are reinforced with hydroxyapatite (HA) nanowires and calcium silicate (CS) nanofibers to achieve the goal. The prepared composite cryogel microspheres with open porous structure and rough surface greatly facilitate cell anchoring, simultaneously exhibiting excellent injectability. Compared to the only HA-or CS-containing counterparts, the GelMA cryogel microspheres composited with HA:CS (termed as GMHC) achieve sustained release of bioactive Ca, P, and Si elements, which are conducive to osteogenic differentiation of bone marrow mesenchymal stromal cells (BMSCs). These composite microspheres can prevent from forming peralkalic conditions, which is beneficial for cell growth. After injection of cryogel microspheres into rat calvarial defects, neo-bone tissue grows into their pores, showing tight integration. The embedded bioceramic components significantly promote bone regeneration, with the GMHC achieving the best regenerative outcomes. Promisingly, porous organic−inorganic composite cryogel microspheres, with high specific surface area, biodegradability, and osteoinductivity, can act as injectable microscaffolds to repair bone defects with enhanced efficiency, which may widen the scaffold strategy for bone tissue engineering.

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“…Similarly, CEVs derived from monocytes (MC-EVs) induced the up-regulated expression of osteogenic-related genes in MSCs [ 88 ]. Schwann cell-derived CEVs (SC-EVs) promoted BMMSC osteogenic differentiation through TGF-β1/SMAD2/3 signaling pathway, M2 macrophage polarization, vascularization, nerve repair, and ultimately bone defect restoration [ 89 ]. Inflammatory osteoclast (iOC)-derived CEVs (iOC-EVs) have been proved to reduce the Osterix ubiquitination in MC3T3-E1 cells by transferring lncRNA LIOCE, thus facilitating bone regeneration [ 90 ].…”

Section: Cevs and Periodontal Regenerationmentioning

confidence: 99%

“…The adverse effects of M-EVs on HUVEC function under certain conditions have ever been reported. However, M2-EVs could inhibit inflammation and apoptosis of HUVECs, and promote their functions by delivering miR-221-3p and miR-21 activating related signaling pathways [ 89 , 125 , 126 ]. Therefore, the degree of vascularization of tissues or scaffolds should be taken into consideration in a successful periodontal regeneration strategy.…”

Section: Cevs and Periodontal Regenerationmentioning

confidence: 99%

The Applications and Potentials of Extracellular Vesicles from Different Cell Sources in Periodontal Regeneration

Huang

Wang

et al. 2023

IJMS

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Periodontitis is a chronic infectious disease worldwide that can cause damage to periodontal supporting tissues including gingiva, bone, cementum and periodontal ligament (PDL). The principle for the treatment of periodontitis is to control the inflammatory process. Achieving structural and functional regeneration of periodontal tissues is also essential and remains a major challenge. Though many technologies, products, and ingredients were applied in periodontal regeneration, most of the strategies have limited outcomes. Extracellular vesicles (EVs) are membranous particles with a lipid structure secreted by cells, containing a large number of biomolecules for the communication between cells. Numerous studies have demonstrated the beneficial effects of stem cell-derived EVs (SCEVs) and immune cell-derived EVs (ICEVs) on periodontal regeneration, which may be an alternative strategy for cell-based periodontal regeneration. The production of EVs is highly conserved among humans, bacteria and plants. In addition to eukaryocyte-derived EVs (CEVs), a growing body of literature suggests that bacterial/plant-derived EVs (BEVs/PEVs) also play an important role in periodontal homeostasis and regeneration. The purpose of this review is to introduce and summarize the potential therapeutic values of BEVs, CEVs and PEVs in periodontal regeneration, and discuss the current challenges and prospects for EV-based periodontal regeneration.

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A multifunctional neuromodulation platform utilizing Schwann cell-derived exosomes orchestrates bone microenvironment via immunomodulation, angiogenesis and osteogenesis

Cited by 27 publications

References 65 publications

Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair

Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair

Injectable, High Specific Surface Area Cryogel Microscaffolds Integrated with Osteoinductive Bioceramic Fibers for Enhanced Bone Regeneration

The Applications and Potentials of Extracellular Vesicles from Different Cell Sources in Periodontal Regeneration

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