A multifunctional molecularly imprinted polymer-based biosensor for direct detection of doxycycline in food samples

Abstract

In this study, we developed a new type of multifunctional molecularly imprinted polymer (MIP) composite as an all-in-one biosensor for the low-cost, rapid and sensitive detection of doxycycline in pig plasma. The MIP composite consisted of a magnetic core for ease of manipulation, and a shell of fluorescent MIPs for selective recognition of doxycycline. By simply incorporating a small amount of fluorescent monomer (fluorescein-O-acrylate), the fluorescent MIP layer was successfully grafted onto the magnetic core via a surface imprinting technique. The resultant MIP composites showed significant doxycycline-dependent fluorescence quenching in an aqueous environment. Good linearity ranging from 0.2 to 6 µM was achieved, and the limit of detection was determined to be 117 nM. The biosensor also showed good selectivity towards doxycycline when compared to other common antibiotic residues. The multifunctional MIP composites were used to directly extract doxycycline from spiked pig plasma samples and quantify the antibiotics based on the quenched fluorescence signals. Recoveries of doxycycline were found in the range of 88-107%.

Green synthesis as a simple and rapid route to protein modified magnetic nanoparticles for use in the development of a fluorometric molecularly imprinted polymer-based assay for detection of myoglobin

“…Adding a fluorescent monomer during MIP synthesis is potentially an easy way to prepare a fluorescent MIP (F-MIP). For example, Ashley et al (2018) used fluorescein- O- acrylate as the fluorescent tag, with fluorescent quenching used in the detection of low molecular weight doxycycline [ 27 , 33 ]. This suggests that using a fluorescent tag with a MIP imprinted for a biomarker could be a successful approach.…”

Fluorescent Nanosensor Based on Molecularly Imprinted Polymers Coated on Graphene Quantum Dots for Fast Detection of Antibiotics

“…They have many advantages, such as short analysis time, ease of use, and small sample volume. One simple way to prepare fluorescent MIPs is adding fluorescent monomers to MIP during synthesis [ 14 , 15 ]. However, fluorescence monomer normally involves complicated synthesis work.…”

The Use of Aptamers and Molecularly Imprinted Polymers in Biosensors for Environmental Monitoring: A Tale of Two Receptors

“…This technique is no longer commonly used, with researchers opting for the non-covalent imprinting strategies. MIPs can be synthesized into a number of different morphologies, including bulk materials, nanomaterials, nanocomposites, or thin films [13][14][15]. Traditionally, MIPs were formed using bulk polymerization as a sorbent for solid-phase extraction via the formation of large size MIP monoliths.…”