A multidrug and toxic compound extrusion (MATE) transporter modulates auxin levels in root to regulate root development and promotes aluminium tolerance

Abstract

MATE (multidrug and toxic compound extrusion) transporters play multiple roles in plants including detoxification, secondary metabolite transport, aluminium (Al) tolerance, and disease resistance. Here we identify and characterize the role of the Arabidopsis MATE transporter DETOXIFICATION30. AtDTX30 regulates auxin homeostasis in Arabidopsis roots to modulate root development and Al‐tolerance. DTX30 is primarily expressed in roots and localizes to the plasma membrane of root epidermal cells including root hairs. dtx30 mutants exhibit reduced elongation of the primary root, root hairs, and lateral roots. The mutant seedlings accumulate more auxin in their root tips indicating role of DTX30 in maintaining auxin homeostasis in the root. Al induces DTX30 expression and promotes its localization to the distal transition zone. dtx30 seedlings accumulate more Al in their roots but are hyposensitive to Al‐mediated rhizotoxicity perhaps due to saturation in root growth inhibition. Increase in expression of ethylene and auxin biosynthesis genes in presence of Al is absent in dtx30. The mutants exude less citrate under Al conditions, which might be due to misregulation of AtSTOP1 and the citrate transporter AtMATE. In conclusion, DTX30 modulates auxin levels in root to regulate root development and in the presence of Al indirectly modulates citrate exudation to promote Al tolerance.

MicroRNA Zma-miR528 Versatile Regulation on Target mRNAs during Maize Somatic Embryogenesis

“…(*) Experimentally validated targets for zma-miR528 in a previous report: ZmLAC3 (Zm00001d052243_T001); ZmLAC5 (Zm00001d042901_T001). ( b ) List of the selected potential targets showing the recognition site pairing for zma-miR528 and their reported function in plant pathways according to several reports [ 19 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ].…”

Understanding the Intricate Web of Phytohormone Signalling in Modulating Root System Architecture

“…OsWOX4 regulates this response by direct promoter binding and transcriptional activation of OsAUX1 [ 48 ]. A multidrug and toxic compound extrusion (MATE) transporter also governs root development through the modulation of auxin homeostasis in the roots [ 49 ]. In soybean, GmYUC2a is an important regulator of auxin biosynthesis during root development and nodulation [ 50 ].…”

The role of solute transporters in aluminum toxicity and tolerance

“…Similarly, VuMATE2 also acts as a proton‐coupled citrate permeable channel (Liu et al 2018). Work from our lab showed that an Arabidopsis MATE transporter, AtDTX30, is involved in Al resistance (Upadhyay et al 2020). Though the physiological substrate is still unidentified, AtDTX30 localizes to the distal transition zone of the root upon Al stress and alleviates the toxic response by citrate exudation (Upadhyay et al 2020).…”

“…Work from our lab showed that an Arabidopsis MATE transporter, AtDTX30, is involved in Al resistance (Upadhyay et al 2020). Though the physiological substrate is still unidentified, AtDTX30 localizes to the distal transition zone of the root upon Al stress and alleviates the toxic response by citrate exudation (Upadhyay et al 2020). Several MATE transporters that have been widely characterized for their ability to efflux citrate to confer Al tolerance are listed by Kryvoruchko et al (2018) and Upadhyay et al (2019).…”