A multidisciplinary investigation on the bioavailability and activity of peptides from lupin protein

Peptides derived from lupin proteins confer potent protection against oxidative stress

“…Recent work has shown that γ‐conglutin, a specific hypoglycemic lupin protein, can be absorbed by Caco‐2 cells . A later study has further validated that lupin peptides with specific structures are potentially absorbed in human intestinal cells for maintaining their biological activity . However, how lupin peptides with different ranges of molecular weight (MW) affect the intrinsic antioxidant systems still needs further investigation.…”

Perspectives of lupine wholemeal protein and protein isolates biodegradation

“…As mentioned above, it was demonstrated that tryptic and peptic peptides derived from lupine protein hydrolysis could modulate cholesterol metabolism in HepG2 cells by inhibiting HMGCoAR via a statin‐like mechanism system (Lammi et al ., ). Furthermore, these peptides may be absorbed in the small intestine, as they are transferred from the apical to the basolateral compartment in differentiated Caco‐2 cells grown in a two‐compartment system (Lammi et al ., ). A study in mildly hypercholesterolaemic subjects demonstrated that the level of circulating proprotein convertase subtilisin/kexin type 9 (PCSK9), which regulates low‐density lipoprotein receptor levels and function, was significantly reduced after a 4‐week consumption of a lupine protein isolate (30 g protein d −1 ) (Lammi et al ., ).…”

“…The predominant polypeptides in lupine are a-conglutins ( $ 55 kDa) and b-conglutins ( $ 13 to 80 kDa), which represent two types of globulinscommon storage proteins (Tyl & George, 2017). As mentioned above, it was demonstrated that tryptic and peptic peptides derived from lupine protein hydrolysis could modulate cholesterol metabolism in HepG2 cells by inhibiting HMGCoAR via a statin-like mechanism system (Lammi et al, 2016a). Furthermore, these peptides may be absorbed in the small intestine, as they are transferred from the apical to the basolateral compartment in differentiated Caco-2 cells grown in a two-compartment system (Lammi et al, 2016a).…”

“…Based on the results, we hypothesised that the bioavailability of lupine seed protein could be affected by the activity of trypsin inhibitors, the free amino acids (AAs) profile, biogenic amines (BAs), antioxidant capacity of the substrate, isoflavone content and the molecular weight of new peptides formed and their properties, among others. The tryptic and peptic peptides derived from lupine protein hydrolysis were shown to modulate cholesterol metabolism (Lammi et al ., ,b,c). Due to their health‐enhancing attributes and resultant selective, effective, safe and good tolerance once consumed, plant‐derived bioactive peptides are commonly consumed as part of a healthy, balanced diet and are often incorporated in functional foods, pharmaceuticals and medicines (Hayes & Bleakley, ).…”

YDFYPSSTKDQQS (P3), a peptide from lupin protein, absorbed by Caco-2 cells, modulates cholesterol metabolism in HepG2 cells via SREBP-1 activation

“…Since bioactive peptides must be available in order to express their activity, absorption experiments have been performed using a model of the intestine based on differentiated human intestinal Caco‐2 cells (Ferruzza, Rossi, Scarino, & Sambuy, ; Sambuy et al, ). The analysis of the basolateral solutions, performed by Chip‐HPLC ESI‐MS/MS, permitted the identification of 8 peptic peptides and 11 tryptic peptides that are absorbed (Lammi, Aiello, et al, ). Subsequently, an experiment conducted in a co‐culture system combining Caco‐2 cells and HepG2 cells demonstrated that either the peptic or the tryptic peptides transferred in basolateral chambers were still able to modulate cholesterol metabolism in HepG2 cells, with an intensity that was even improved versus that of the parent hydrolysates by the crosstalk between the two cell systems in co‐culture (Lammi, Aiello, et al, ).…”