A multicystatin is induced by drought‐stress in cowpea (<i>Vigna unguiculata</i> (L.) Walp.) leaves

Diop, Ndèye N.; Kidrič, Marjetka; Repellin, Anne; Gareil, Monique; d’Arcy-Lameta, Agnès; Thi, Anh Thu Pham; Zuily-Fodil, Yasmine

doi:10.1016/j.febslet.2004.10.014

Cited by 95 publications

(62 citation statements)

References 46 publications

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“…Pernas et al 1998). 또한 C 말단 영역에는 2개 의 매우 유사한 보존영역이 존재하며 이들 영역은 토마 토, 사탕수수, 키위, Arabidopsis 등 에서도 잘 알려져 있다 (Belenghi et al 2003;Diop et al 2004;Gianotti et al 2006;Jacinto et al 1998;).…”

Section: Southern Blot 분석unclassified

Isolation and characterization of Brcpi1 gene encoding phytocystatin from chinese cabbage (Brassica rapa L.) seedlings

Jung¹,

Cho²,

Kang³

2009

Journal of Plant Biotechnology

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A cDNA clone encoding phytocystatin was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (name as Brcpi1; GenBank accession no.: EF079953) had a total length of 881 bp with an open reading frame of 609 bp, and encoded predicted polypeptide of 203 amino acid (aa) residues including a putative N-terminal signal peptide. Other relevant regions found its sequence included the G and PW conserved aa motifs, and the consensus LARFAV sequence for phytocystatins and the reactive site QVVAG. The BrCPI1 protein shared 95, 94, 81, 80 and 78% identity with other CPI proterins isolated from Brassica oleracea (BoCPI-1), Arabidopsis thaliana (AtCY SB), Glycine max (GmCPI), Oryza sativa (OsCYS-2) and Zea may (ZmCPI) at amino acid level, respectively. Southern blot analysis showed that Brcpi1 was a low copy gene. Expression pattern analysis revealed that Brcpi1 was a tissue-specific expressing gene during reproductive growth and strongly expressed at mature seedling stages. Furthermore, overexpression of Brcpi1 in transgenic Arabidopsis was enhanced tolerance to salt and cold stresses. Meanwhile the juvenile seedling of Brcpi1 transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that Brcpi1 functioned as a cysteine protease inhibitor and it exhibited a protective agent against diverse types of abiotic stress, which induced this gene in a tissue-and stress-specific manner.

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Section: Southern Blot 분석unclassified

Isolation and characterization of Brcpi1 gene encoding phytocystatin from chinese cabbage (Brassica rapa L.) seedlings

Jung¹,

Cho²,

Kang³

2009

Journal of Plant Biotechnology

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“…Among the presumably complete cystatins, the main body of cowpea (Vigna unguiculata) CPI-1 (Diop et al, 2004;Martínez et al, 2005b) was most similar to AcCYS1. However, CPI-1 has only six amino acids between the predicted signal peptide processing site and the main body (Fig.…”

Section: Accys1 Contains a Signal Peptide And An Extended Ntt Rich Inmentioning

confidence: 99%

“…Some phytocystatins were predicted to possess an N-terminal signal peptide for transport into the lumen of the endoplasmic reticulum and/or a C-terminal extension, which may be involved in binding legumaintype Cys proteases (Lim et al, 1996;Womack et al, 2000;Martínez et al, 2005aMartínez et al, , 2007Abraham et al, 2006;Gianotti et al, 2006). Other phytocystatins, designated multicystatins, contain multiple copies of the main body (Kouzuma et al, 2000;Diop et al, 2004;Christova et al, 2006;Girard et al, 2007).…”

Section: Lvi]-[agt]-[rke]-[fy]-[as]-[vi]-x-[edqv]-[hyfq]mentioning

confidence: 99%

An Extended AE-Rich N-Terminal Trunk in Secreted Pineapple Cystatin Enhances Inhibition of Fruit Bromelain and Is Posttranslationally Removed during Ripening

2009

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Phytocystatins are potent inhibitors of cysteine proteases and have been shown to participate in senescence, seed and organ biogenesis, and plant defense. However, phytocystatins are generally poor inhibitors of the cysteine protease, bromelain, of pineapple (Ananas comosus). Here, we demonstrated that pineapple cystatin, AcCYS1, inhibited (.95%) stem and fruit bromelain. AcCYS1 is a unique cystatin in that it contains an extended N-terminal trunk (NTT) of 63 residues rich in alanine and glutamate. A signal peptide preceding the NTT is processed in vitro by microsomal membranes giving rise to a 27-kD species. AcCYS1 mRNA was present in roots and leaves but was most abundant in fruit. Using immunofluorescence and immunoelectron microscopy with an AcCYS1-specific antiserum, AcCYS1 was found in the apoplasm. Immunoblot analysis identified a 27-kD protein in fruit, roots, and leaves and a 15-kD species in mature ripe fruit. Ripe fruit extracts proteolytically removed the NTT of 27-kD AcCYS1 in vitro to produce the 15-kD species. Mass spectrometry analysis was used to map the primary cleavage site immediately after a conserved critical glycine-94. The AE-rich NTT was required to inhibit fruit and stem bromelain (.95%), whereas its removal decreased inhibition to 20% (fruit) and 80% (stem) and increased the dissociation equilibrium constant by 1.8-fold as determined by surface plasmon resonance assays. We propose that proteolytic removal of the NTT results in the decrease of the inhibitory potency of AcCYS1 against fruit bromelain during fruit ripening to increase tissue proteolysis, softening, and degradation.

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“…Moreover, the most recent findings signify the involvement of abiotic environmental stresses such as drought and salt in phytocystatins induction (Gaddour et al 2001;Corre-Menguy et al 2002;Diop et al 2004;Petya et al 2007;Girard et al 2007). Despite these recent extensive studies on phytocystatins, our knowledge regarding their expression as recombinant or fusion products is still poor (Gianotti et al 2006;Sugawara et al 2002;Oliva et al 2004).…”

Section: -[Agt]-[rke]-[fy]-[as]-[vi]-x-[edqv]-[hyfq]-n In Their N-termentioning

confidence: 99%

Molecular analysis of maize cystatin expression as fusion product in Escherichia coli

Gholizadeh

2012

Physiol Mol Biol Plants

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Nowadays, plant cysteine proteinase inhibitors "namely phytocystatins" have attracted researchers towards the identification of their molecular structures and novel physiological functions. Their important roles in plant developmental processes and different stress responses have been well known. In spite of advances in the understanding of phytocystatins, we lack enough data concerning their heterologous expression especially in the forms of fusion products that are most important whether for biochemical, pharmacological or clinical studies. The present work describes an easy method of expression, purification and functional characterization in Escherichia coli of maize cystatin as a part of maltosebinding fusion protein. Assessments revealed that upon expression of fused product the total antioxidation status of the induced recombinant cells is increased. This result leads to question 'Is there any parallel functional correlation between anti-proteolytic and anti-oxidative systems?' However, the present research will open a gate for the new studies regarding the putative communicative roles of these systems that may be existing in the biological world.

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A multicystatin is induced by drought‐stress in cowpea (Vigna unguiculata (L.) Walp.) leaves

Cited by 95 publications

References 46 publications

Isolation and characterization of Brcpi1 gene encoding phytocystatin from chinese cabbage (Brassica rapa L.) seedlings

Isolation and characterization of Brcpi1 gene encoding phytocystatin from chinese cabbage (Brassica rapa L.) seedlings

An Extended AE-Rich N-Terminal Trunk in Secreted Pineapple Cystatin Enhances Inhibition of Fruit Bromelain and Is Posttranslationally Removed during Ripening

Molecular analysis of maize cystatin expression as fusion product in Escherichia coli

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