A multicopper oxidase is essential for manganese oxidation and laccase‐like activity in <i>Pedomicrobium</i> sp. ACM 3067

Ridge, Justin P.; Lin, Marianne; Larsen, Eloise; Fegan, Mark; McEwan, Alastair G.; Sly, Lindsay I.

doi:10.1111/j.1462-2920.2006.01216.x

Cited by 125 publications

(95 citation statements)

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“…Manganese is used as a reliable tracer of hydrothermal vent emissions (44)(45)(46) and, unlike the other vibrios, the genome of V. antiquarius contains a gene annotated as multicopper oxidase, an enzyme essential for manganese oxidation, and laccase-like activity (47). V. antiquarius also contains delta-9 fatty acid desaturase.…”

Section: Resultsmentioning

confidence: 99%

Deep-sea hydrothermal vent bacteria related to human pathogenic Vibrio species

Hasan

Grim

Lipp

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Vibrio species are both ubiquitous and abundant in marine coastal waters, estuaries, ocean sediment, and aquaculture settings worldwide. We report here the isolation, characterization, and genome sequence of a novel Vibrio species, Vibrio antiquarius, isolated from a mesophilic bacterial community associated with hydrothermal vents located along the East Pacific Rise, near the southwest coast of Mexico. Genomic and phenotypic analysis revealed V. antiquarius is closely related to pathogenic Vibrio species, namely Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio harveyi, and Vibrio vulnificus, but sufficiently divergent to warrant a separate species status. The V. antiquarius genome encodes genes and operons with ecological functions relevant to the environment conditions of the deep sea and also harbors factors known to be involved in human disease caused by freshwater, coastal, and brackish water vibrios. The presence of virulence factors in this deep-sea Vibrio species suggests a far more fundamental role of these factors for their bacterial host. Comparative genomics revealed a variety of genomic events that may have provided an important driving force in V. antiquarius evolution, facilitating response to environmental conditions of the deep sea.W ith more than 110 recognized species, the genus Vibrio comprises a diverse group of heterotrophic bacteria, of which many are known pathogens, causing disease in animals and humans (1, 2). Vibrio cholerae is the most notorious because it is the causative agent of cholera. Vibrio vulnificus and Vibrio parahaemolyticus cause severe illness in humans and are associated with consumption of contaminated seafood (3, 4). Vibrio harveyi (5), Vibrio anguillarum (6, 7), and V. parahaemolyticus (8) continue to cause substantial economic losses to the aquaculture industry worldwide.Vibrios demonstrate a wide range of niche specialization: for example, free-living, attached to biotic and abiotic surfaces, and resident in both estuarine and marine habitats (9). The deep sea constitutes the largest habitat of the biosphere that supports microbial communities across three domains of life and represents an environment where physiochemical parameters-such as low temperature, high salinity, and high pressure-modulate community structure (10, 11). Several studies have shown the presence of physiologically, metabolically, and phylogenetically diverse mesophilic microbial communities in the deep sea, including Vibrio species (12-15). Barotolerant Vibrio spp. have been isolated from deep-sea sediment and from the gut microflora of invertebrates and fish collected from a variety of deepsea habitats, including hydrothermal vents (16,17). For example, strains of Vibrio, Aeromonas, and Pseudomonas spp. were isolated from mud-water samples collected at a depth of 4,940 m, 150 miles east of Cape Canaveral, Florida (18). Several culturedependent and -independent studies have confirmed the ubiquity of vibrios, and suggested Vibrio populations generally comprise approximately 1% (by molecular...

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Section: Resultsmentioning

confidence: 99%

Deep-sea hydrothermal vent bacteria related to human pathogenic Vibrio species

Hasan

Grim

Lipp

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…Recently, a fourth MCO has been shown to be required for Mn (II) oxidation in the α -proteobacterium Pedomicrobium sp. ACM 3067 (Ridge et al 2007). In all four of these model organisms these MCOs are hypothesized to encode the catalytic Mn (II) oxidase.…”

Section: Introductionmentioning

confidence: 99%

“…Studies on Mn(II) oxidation in α-proteobacteria have been limited, yet the effect of copper on Mn(II) oxidation in whole cells (Larsen et al 1999), the non Mn(II) oxidizing phenotype of a MCO mutant (Ridge et al 2007), and the effect of copper chelators on Mn(II) oxidation in acrylamide gels (Francis et al 2001) suggest copper may play an important role in Mn(II) oxidation in this phylogenetic group. The marine α-proteobacterium Erythrobacter sp.…”

Section: Introductionmentioning

confidence: 99%

In vitro studies indicate a quinone is involved in bacterial Mn(II) oxidation

Johnson

Tebo

2007

Arch Microbiol

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Manganese(II)-oxidizing bacteria play an integral role in the cycling of Mn as well as other metals and organics. Prior work with Mn(II)-oxidizing bacteria suggested that Mn(II) oxidation involves a multicopper oxidase, but whether this enzyme directly catalyzes Mn(II) oxidation is unknown. For a clearer understanding of Mn(II) oxidation, we have undertaken biochemical studies in the model marine α-proteobacterium, Erythrobacter sp. strain SD21. The optimum pH for Mn(II)-oxidizing activity was 8.0 with a specific activity of 2.5 nmol × min −1 × mg −1 and a K m = 204 µM. The activity was soluble suggesting a cytoplasmic or periplasmic protein. Mn(III) was an intermediate in the oxidation of Mn(II) and likely the primary product of enzymatic oxidation. The activity was stimulated by pyrroloquinoline quinone (PQQ), NAD + , and calcium but not by copper. In addition, PQQ rescued Pseudomonas putida MnB1 non Mn(II)-oxidizing mutants with insertions in the anthranilate synthase gene. The substrate and product of anthranilate synthase are intermediates in various quinone biosyntheses. Partially purified Mn(II) oxidase was enriched in quinones and had a UV/VIS absorption spectrum similar to a known quinone requiring enzyme but not to multicopper oxidases. These studies suggest that quinones may play an integral role in bacterial Mn(II) oxidation.

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“…SG-1, Leptothrix discophorastrain SS-1 and SP6, Pseudomonas putida strains MnB1 and GB-1, and Pedomicrobium sp. ACM 3067 (Brouwers et al, 2000;Geszvain et al, 2013;Ridge et al, 2007;Saratovsky et al, 2006;Villalobos et al, 2003), fungi Acremonium, Paraconiothyrium, Phanerochaete, Cephalosporiumsp., Coniothyrium, Periconia sp., Sporothrix sp., Phoma (Santucci et al, 2000, Sasaki et al, 2006Saratovsky et al, 2009;Timonin et al, 1972) and algae like Gloeothece Magna and Cladophora (Duggan et al, 1992;Mohamed, 2001) play an important role in the transformation and speciation of manganese in the environment along with the abiotic processes. Manganese biogenesis has been extensively characterized in bacterial systems.…”

Section: Biotic Speciationmentioning

confidence: 99%

Manganese: Its Speciation, Pollution and Microbial Mitigation

Sinha¹,

Khare²

2013

Int J Appl Sci Biotechnol

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Manganese is known to be one of the essential trace elements and has plenty of applications. Inspite of its essential nature, concerns have arising due to its toxic nature at higher concentration. Several methods of removing manganese from environment have been proposed during the last few decades. However, the most favourable option based on cost-effectiveness, performance, and simplicity is still under investigation. The current review summarizes updated information on various technical aspects on manganese, including chemical nature, speciation, toxicity and remediation strategies. The review starts with covering the major sources of manganese, its interaction with biological biomolecules causing toxicity. This is followed by its speciation in environment, describing both biotic and abiotic processes. The biotic processes describe the role of microorganisms in the oxidation/ reduction of various oxidation states of manganese. Whereas, abiotic processes mainly describes the role of pH and oxygen taking thermodynamical aspects. The main part of this review focuses on recent developments on using microbial systems for manganese bioremediation. The updated works on different strategies adopted for the remediation of manganese from the environment have also been summarized.

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A multicopper oxidase is essential for manganese oxidation and laccase‐like activity in Pedomicrobium sp. ACM 3067

Cited by 125 publications

References 46 publications

Deep-sea hydrothermal vent bacteria related to human pathogenic Vibrio species

Deep-sea hydrothermal vent bacteria related to human pathogenic Vibrio species

In vitro studies indicate a quinone is involved in bacterial Mn(II) oxidation

Manganese: Its Speciation, Pollution and Microbial Mitigation

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