A multicolor multiplex lateral flow assay for high-sensitivity analyte detection using persistent luminescent nanophosphors

Danthanarayana, Adheesha N.; Finley, Erin; Vu, Binh; Kourentzi, Katerina; Willson, Richard C.; Brgoch, Jakoah

doi:10.1039/c9ay02247c

Cited by 38 publications

(33 citation statements)

References 39 publications

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“…Based on their previous work, [102] Brgoch et al designed multicolor LFA using PLNPs with blue and green emission as labels to simultaneously detect PSA and HCG analytes using smartphone analyzing system. [103] Lv and co-workers prepared three kinds of PLNPs with ratiometric dual-emission persistent luminescence using template method. The luminescence of their PLNPs was quenched when integrated with specific metal ions, while the existence of bio-thiols led to regeneration of luminescence.…”

Section: Pattern Signal-based Materialsmentioning

confidence: 99%

Functional Micro‐/Nanomaterials for Multiplexed Biodetection

Liu

Cui

et al. 2021

Advanced Materials

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When analyzing biological phenomena and processes, multiplexed biodetection has many advantages over single-factor biodetection and is highly relevant to both human health issues and advancements in the life sciences. However, many key problems with current multiplexed biodetection strategies remain unresolved. Herein, the main issues are analyzed and summarized: 1) generating sufficient signal to label targets, 2) improving the signal-to-noise ratio to ensure total detection sensitivity, and 3) simplifying the detection process to reduce the time and labor costs of multiple target detection. Then, available solutions made possible by designing and controlling the properties of micro-and nanomaterials are introduced. The aim is to emphasize the role that micro-/nanomaterials can play in the improvement of multiplexed biodetection strategies. Through analyzing existing problems, introducing state-of-the-art developments regarding relevant materials, and discussing future directions of the field, it is hopeful to help promote necessary developments in multiplexed biodetection and associated scientific research.

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Section: Pattern Signal-based Materialsmentioning

confidence: 99%

Functional Micro‐/Nanomaterials for Multiplexed Biodetection

Liu

Cui

et al. 2021

Advanced Materials

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“…[ 175 ] For example, the most popular format used in POC is the LFA with multitest lines. [ 176–178 ] However, multiplexing in LFAs is a challenge due to the confined small sensing domain and thus the limited sensitivity. Combining with advances in dispensing technologies and assay development, a LFA was able to detect seven analytes in a single test strip by dispensing picolitre sensor on the sensing zone to achieve molecular encoding of analytes.…”

Section: Challenges and Perspectives For Poc Detection Of Cytokinesmentioning

confidence: 99%

Point‐of‐care detection of cytokines in cytokine storm management and beyond: Significance and challenges

Liu

Jiang

Lin

et al. 2021

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Cytokines are signaling molecules between cells in immune system. Cytokine storm, due to the sudden acute increase in levels of pro‐inflammatory circulating cytokines, can result in disease severity and major‐organ damage. Thus, there is urgent need to develop rapid, sensitive, and specific methods for monitoring of cytokines in biology and medicine. Undoubtedly, point‐of‐care testing (POCT) will provide clinical significance in disease early diagnosis, management, and prevention. This review aims to summarize and discuss the latest technologies for detection of cytokines with a focus on POCT. The overview of diseases resulting from imbalanced cytokine levels, such as COVID‐19, sepsis and other cytokine release syndromes are presented. The clinical cut‐off levels of cytokine as biomarkers for different diseases are summarized. The challenges and perspectives on the development of cytokine POCT devices are also proposed and discussed. Cytokine POCT devices are expected to be the ongoing spotlight of disease management and prevention during COVID‐19 pandemic and also the post COVID‐19 pandemic era.

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“…Similarly, the integration of core–shell upconversion NPs and LFA resulted in higher sensitivity of analyte detection that is based on specific fluorescence ratiometric evaluation of analyte (Ji et al., 2019). The multiple analyte detection on a single strip is also possible by using two persistent luminescent nanophosphors that emit different color signals (Danthanarayana et al., 2020). Multicolor AuNPs (blue and red) are also used for development of multiplex LFA with single T‐line (Di Nardo et al., 2019).…”

Section: Poc Testing Devicesmentioning

confidence: 99%

Rapid point‐of‐care testing methods/devices for meat species identification: A review

Kumar

Narsaiah

2020

Comp Rev Food Sci Food Safe

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The authentication of animal species is an important issue due to an increasing trend of adulteration and mislabeling of animal species in processed meat products. Polymerase chain reaction is the most sensitive and specific technique for nucleic acid‐based animal species detection. However, it is a time‐consuming technique that requires costly thermocyclers and sophisticated labs. In recent times, there is a need of on‐site detection by point‐of‐care (POC) testing methods and devices under low‐resource settings. These POC devices must be affordable, sensitive, specific, user‐friendly, rapid and robust, equipment free, and delivered to the end users. POC devices should also confirm the concept of micro total analysis system. This review discusses POC testing methods and devices that have been developed for meat species identification. Recent developments in lateral flow assay‐based devices for the identification of animal species in meat products are also reviewed. Advancements in increasing the efficiency of lateral flow detection are also discussed.

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A multicolor multiplex lateral flow assay for high-sensitivity analyte detection using persistent luminescent nanophosphors

Abstract: Multicolor multiplex lateral flow assay using persistent luminescent nanophosphors as reporters.

Cited by 38 publications

References 39 publications

Functional Micro‐/Nanomaterials for Multiplexed Biodetection

Functional Micro‐/Nanomaterials for Multiplexed Biodetection

Point‐of‐care detection of cytokines in cytokine storm management and beyond: Significance and challenges

Rapid point‐of‐care testing methods/devices for meat species identification: A review

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