A multicolor flow cytometric assay for measurement of platelet-derived microparticles

Mobarrez, Fariborz; Antović, Jovan P.; Egberg, Nils; Hansson, Mona; Jörneskog, Gun; Hultenby, Kjell; Wallén, Håkan

doi:10.1016/j.thromres.2009.10.006

Cited by 109 publications

(110 citation statements)

References 19 publications

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“…We used lactadherin to detect PS exposure, a method which has been shown to have good sensitivity and specificity. 12 However, we observed a relatively large number of lactadherin negative particles in the patient group when compared with controls. To explain this phenomenon, further research is needed.…”

Section: Discussionmentioning

confidence: 51%

“…MPs were defined as particles < 1.0 mm in size, negative to phalloidin-Alexa 660 (cell-fragment marker, Invitrogen, Paisley, UK), 12 immunoglobulins (IgG1-FITC, IgG1-PE, IgG1-PC7 and IgG1-APC) with no reactivity against human antigens were used as negative controls. In the present study results are shown as both absolute numbers of MP (MP counted Â standard beads added/L)/standard beads counted (FlowCount, Beckman Coulter, CA, USA).…”

mentioning

confidence: 99%

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Studies of microparticles in patients with the antiphospholipid syndrome (APS)

Vikerfors

Mobarrez

Bremme

et al. 2012

Lupus

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Objectives: To study circulating platelet, monocyte and endothelial microparticles (PMPs, MMPs and EMPs) in patients with antiphospholipid syndrome (APS) in comparison with healthy controls. Material and method: Fifty-two patients with APS and 52 healthy controls were investigated. MPs were measured on a flow cytometer (Beckman Gallios) and defined as particles sized < 1.0 mm, negative to phalloidin, positive to lactadherin and positive to either CD42a (PMPs), CD144 (EMPs) or CD14 (MMPs). Exposure of CD142 (TF) was measured on CD144 positive MPs. Results: Total number of MPs (i.e. lactadherin positive particles) was higher in APS patients versus controls (p < 0.001). An increased number of EMPs (p < 0.001), increased TF-positive EMPs (p < 0.001) and increased MMPs (p < 0.001) were also observed. PMP numbers did not differ between the groups. None of the MP types differed in numbers between obstetric and thrombotic APS patients. Conclusion: We observed a high number of EMPs expressing TF in APS patients. The numbers of MMPs and total EMPs were also higher as compared with healthy controls but in contrast to previous reports, the number of PMPs did not differ between groups. Lupus (2012) 21, 802-805.

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Section: Discussionmentioning

confidence: 51%

mentioning

confidence: 99%

Studies of microparticles in patients with the antiphospholipid syndrome (APS)

Vikerfors

Mobarrez

Bremme

et al. 2012

Lupus

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show abstract

“…In platelet-poor plasma obtained from healthy donors, 80% of platelet-derived MPs failed to bind annexin V (Connor et al, 2010). In this case, a phalloidin-staining of actin filaments could be helpful in discrimination of MPs and other cell fragments (Mobarrez et al, 2010). Washing samples as well as double centrifugation result in decreased annexin-V (Ayers et al 2011).…”

Section: Specific Antigens In Mp Discriminationmentioning

confidence: 99%

Acceleration of New Biomarkers Development and Discovery in Synergistic Diagnostics of Coronary Artery Disease

Stępień¹

2011

Coronary Angiography - Advances in Noninvasive Imaging Approach for Evaluation of Coronary Artery Disease

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“…For instance, platelet-derived MVs expose platelet membrane glycoprotein Ib (GPIb; CD42b), platelet-endothelium adhesion molecule-1 (PECAM-1; CD31), P-selectin (CD62P), CD36 [19] , and the integrin αIIbβ3 (GPIIb-IIIa) [20] . The monocyte-derived MVs expose CD14, while the endothelialderived MVs expose CD31, CD34, CD51, CD62E, CD144, and CD146 [17,21] .…”

Section: General Characteristics Of Mvsmentioning

confidence: 99%

“…In vivo studies, conducting with mouse or monkey under intravital microscopy, have shown that the TF + MVs are incorporated into the thrombus formation [40] . The appearance of P-selectin [20] , PSGL-1, glycoprotein (GP) IIb/IIIa [41] , protein disulfide isomerase [42] , and factor VIII and factor Va [43] receptors on MVs may also [44] . However, the lack of an association between the increased phospholipid composition of circulating MVs and coagulation has also been reported [45] .…”

Section: Mvs and Thrombosismentioning

confidence: 99%