A multicenter study on the precision and accuracy of homogeneous assays for LDL-cholesterol: Comparison with a beta-quantification method using fresh serum obtained from non-diseased and diseased subjects

Miida, Takashi; Nishimura, Kunihiro; Okamura, Tomonori; Hirayama, Satoshi; Ohmura, Hirotoshi; Yoshida, Hiroshi; Miyashita, Yoh; Ai, Masumi; Tanaka, Akira; Sumino, Hiroyuki; Murakami, Masami; Inoue, Ituro; Kayamori, Yuzo; Nakamura, Masakazu; Nobori, Tsutomu; Miyazawa, Yukihisa; Teramoto, Tamio; Yokoyama, Shinji

doi:10.1016/j.atherosclerosis.2012.08.022

Cited by 61 publications

(58 citation statements)

References 25 publications

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“…The accumulated data showed a similar increasing trend in the levels of Second, all methods of HDL-C measurement used by the SRL were validated using reference methods based on strictly standardized beta-quantification by the CDC/CRMLN Lipid Reference Laboratory. The betaquantification HDL-C data obtained in the CDC/ CRMLN Lipid Reference Laboratory in 2011 7) are consistent with the "elevated values" observed in the long-term increasing trend (Fig. 2); therefore, the HDL-C levels appear to be currently high in Japan.…”

Section: Discussionsupporting

confidence: 68%

“…2). The HDL-C data obtained using beta-quantification in 2011 7) yielded "elevated values" in the blood samples of patients without lipid disorders (serum total cholesterol ＜250 mg/dL and triglycerides ＜150 mg/dL) consistent with a long-term increasing trend (Fig. 2).…”

Section: Discussionmentioning

confidence: 95%

“…Follow-up data obtained in the health checkup program are available at Chugoku Rosai (Labor Welfare) Hospital in Hiroshima, without standardization by the CDC/CRMLN. The HDL-C data obtained in the beta quantification procedure conducted in the CDC/CRMLN Lipid Reference Laboratory in 2011 were also used 7) . Historic data are also available in the clinical laboratories of a few other …”

Section: Sources Of Data For the Analysismentioning

confidence: 99%

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High-Density Lipoprotein Levels Have Markedly Increased Over the Past Twenty Years in Japan

Yokoyama

Ueshima

Miida

et al. 2014

JAT

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Aim:The high-density lipoprotein cholesterol (HDL-C) level is a major negative risk factor for atherosclerotic diseases dependent on various lifestyle parameters. Changes in the lifestyle of Japanese individuals over the past several decades is believed to have increased their total cholesterol levels and the incidence of cardiovascular disease in Japan. It is therefore important to assess the long-term trends in the HDL-C levels with respect to public health in the community. Methods: In this study, accumulated data for the serum/plasma HDL-C levels published in cohort studies and obtained during health checkup programs in Japan were analyzed with respect to timedependent changes. Results: The levels of HDL-C have continuously and significantly increased over the past 20 years by 12-15% according to the National Health and Nutrition Study, other cohort studies and commercially available data. On the other hand, the non-HDL-cholesterol levels demonstrated no changes or only a slight decrease during the same period. This finding is consistent with several sets of data obtained from health checkup programs. The commercially measured levels of serum apoA-I, an independent parameter of serum HDL, also showed a similar long-term increase, supporting the above findings. Conclusion: We concluded that the serum/plasma HDL concentrations in Japanese individuals, selectively, have increased continuously and significantly over the past 20 years or more. The reasons for this phenomenon and the consequent public health outcomes have yet to be investigated. J Atheroscler Thromb, 2014; 21:151-160.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 95%

Section: Sources Of Data For the Analysismentioning

confidence: 99%

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High-Density Lipoprotein Levels Have Markedly Increased Over the Past Twenty Years in Japan

Yokoyama

Ueshima

Miida

et al. 2014

JAT

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“…Previously, we confirmed the precision of these assays. 20,21 High-sensitivity C-reactive protein (hs-CRP) was measured using latex-enhanced immunonephelometry (CardioPhase hsCRP; Siemens Healthcare Diagnostics, NY, USA).…”

Section: Other Laboratory Testsmentioning

confidence: 99%

The bromocresol green assay, but not the modified bromocresol purple assay, overestimates the serum albumin concentration in nephrotic syndrome through reaction with α₂-macroglobulin

et al. 2015

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Background: The bromocresol green (BCG) assay is commonly used for measuring albumin (ALB), but is affected by 1 -and 2 -globulins, which are elevated in systemic inflammation. The modified bromocresol purple (mBCP) assay is another dye-binding method developed to overcome non-specific reactions. Concentrations of 2 -macroglobulin, a major 2 -globulin component, are increased in nephrotic syndrome (NS), but not in inflammation. There is little direct evidence that 2 -macroglobulin affects BCG or mBCP assays. Methods: We measured serum albumin concentrations in 33 patients with NS and 13 reference healthy controls using BCG (ALB BCG ) and mBCP (ALB mBCP ) assays, and nephelometry (nALB) as a reference method. We also determined five specific proteins belonging to the 1 -and 2 -globulins by nephelometry. After adding purified 2 -macroglobulin to albumin solutions, protein reactivity in these three assays was compared. Results: Nephrotic syndrome patients were categorized to tertiles according to nALB concentration. In all tertiles, ALB BCG was significantly higher than nALB, especially in the severe hypoalbuminemia group, in which 2 -macroglobulin was 43-49% higher. By contrast, ALB mBCP and nALB were almost identical in all three groups. The difference between ALB BCG and nALB was positively correlated with the 2 -macroglobulin concentration. In vitro, when 2 -macroglobulin was added to solutions containing identical albumin concentrations, 2 -macroglobulin dose-dependently increased ALB BCG , but not ALB mBCP . Conclusions: In NS, 2 -macroglobulin is a major factor for positive bias of ALB BCG , especially in patients with severe hypoalbuminemia. The mBCP assay is useful for measuring albumin concentrations in NS.

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“…4 As we demonstrate in Table 1, the formula revealed equally lower LDL-c results with respect to lower, medium or higher HDL-c ranges, compared to Friedewald's values. Hence, the differences stated by Laserna-Mendieta and Timon-Zapata in the Spanish study, a general overestimation of LDL-c and an underestimation in extreme HDL-c values, might be explained by a possible bias in their own HDL-c method or calibration.…”

mentioning

confidence: 71%

There appears to be a discrepancy in the units of measurement in the article by Cordova and Cordova on their proposed new formula for LDL-cholesterol estimation

Earp

2013

Ann Clin Biochem

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A multicenter study on the precision and accuracy of homogeneous assays for LDL-cholesterol: Comparison with a beta-quantification method using fresh serum obtained from non-diseased and diseased subjects

Cited by 61 publications

References 25 publications

High-Density Lipoprotein Levels Have Markedly Increased Over the Past Twenty Years in Japan

High-Density Lipoprotein Levels Have Markedly Increased Over the Past Twenty Years in Japan

The bromocresol green assay, but not the modified bromocresol purple assay, overestimates the serum albumin concentration in nephrotic syndrome through reaction with α₂-macroglobulin

There appears to be a discrepancy in the units of measurement in the article by Cordova and Cordova on their proposed new formula for LDL-cholesterol estimation

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A multicenter study on the precision and accuracy of homogeneous assays for LDL-cholesterol: Comparison with a beta-quantification method using fresh serum obtained from non-diseased and diseased subjects

Cited by 61 publications

References 25 publications

High-Density Lipoprotein Levels Have Markedly Increased Over the Past Twenty Years in Japan

High-Density Lipoprotein Levels Have Markedly Increased Over the Past Twenty Years in Japan

The bromocresol green assay, but not the modified bromocresol purple assay, overestimates the serum albumin concentration in nephrotic syndrome through reaction with α2-macroglobulin

There appears to be a discrepancy in the units of measurement in the article by Cordova and Cordova on their proposed new formula for LDL-cholesterol estimation

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The bromocresol green assay, but not the modified bromocresol purple assay, overestimates the serum albumin concentration in nephrotic syndrome through reaction with α₂-macroglobulin