A multi-target screening analysis in human plasma using fast liquid chromatography-hybrid tandem mass spectrometry (Part II)

Abstract

The new software platform developed for screening and identification of small molecules (SmileMS) allows an easy and reproducible detection of drugs and toxic compounds in blood for general unknown screening. It offers automated generation of reports, which makes the LC-MS/MS easier to use without having specialised skills in mass spectrometry. This LC-MS/MS screening method will be a reliable alternative to the Remedi® instrument in the global process of screening in emergency clinical toxicology laboratories.

Application of liquid chromatography tandem mass spectrometry for the simultaneous quantification of multiple non-opioid drugs in human plasma

“…Recently, the very sensitive and valid platform to analyze the blood level of various endogenous and exogenous chemicals using mass spectrometry has been introduced [7][8][9] . In the connection with this, our system to quantify the most common intoxicated chemicals or drugs will be useful.…”

Multi-Target Screening of Biological Samples Using LC–MS/MS: Focus on Chromatographic Innovations

“…In another study, a UHPLC-MS/MS strategy was implemented for the determination of 97 drugs, drugs of abuse, and metabolites in human plasma samples for clinical purposes [54]. This method was proposed as an alternative to the REMEDi HS instrument from Bio-Rad Laboratories which was widely used in clinical laboratories until the end of 2008, this instrument being not supported anymore [55]. The total analysis time was 15 min using a 100 mm column length packed with 1.9 μm fully porous particles.…”

“…As an example, SmileMS (Geneva Bioinformatics SAm, Basel, Switzerland) is an identification software platform for low molecular weight compounds which sorts the peak intensities of an MS spectrum prior to the correlation between two sorted spectra. A probability that a rank from an experimental spectrum matches a rank from a reference spectrum is then calculated and interpreted [55,98].…”

Online extraction toxicological MS(n) screening system for serum and heparinized plasma and comparison of screening results between plasma and urine in the context of clinical data

“…Viette et al recently published two papers [8,9] using a similar instrumentation as Marquet et al [4], Saint-Marcoux et al [5], Sauvage et al [6] and Dresen et al [7]. The method concentrates on serum and was validated only for this matrix; it is not intended to be used for urine.…”