A Multi-strain Potential Probiotic Formulation of GABA-Producing Lactobacillus plantarum 90sk and Bifidobacterium adolescentis 150 with Antidepressant Effects

Yunes, Roman А.; Полуэктова, Е. У.; Васильева, Е. В.; Odorskaya, Maya V.; Марсова, М. В.; Ковалев, Г. И.; Даниленко, В. Н.

doi:10.1007/s12602-019-09601-1

Cited by 82 publications

(47 citation statements)

References 41 publications

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“…The antioxidant properties of L. brevis 47 f [ 9 , 33 ] strain may not be the only prerequisite for the protective effect observed in the present work. Other mechanisms such as the host immune response or secretion of anti-inflammatory agents should be addressed.…”

Section: Discussionmentioning

confidence: 74%

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The Lactobacillus brevis 47 f Strain Protects the Murine Intestine from Enteropathy Induced by 5-Fluorouracil

et al. 2020

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We report that the results of our study indicate that Lactobacillus brevis 47 f strain isolated from the faeces of a healthy individual prevents the manifestations of experimental mucositis induced by treatment of Balb/c mice with the anticancer drug 5-fluorouracil (5 FU; 100 mg/kg i.p. × 3 days). The presence of damage to the intestine and the colon was determined by a morphometric analysis of specimens including the height of villi, the amount of goblet cells and infiltrating mononuclear cells, and the expression of the proliferative Ki-67 antigen. Changes in the lipid peroxidation in the blood and the intestine were determined by severalfold increase of the concentration of malonic dialdehyde. Oral administration of L. brevis 47 f strain prior to 5 FU decreased the drug-induced morphological and biochemical changes to their respective physiological levels; the ability of intestinal epitheliocytes to express Ki-67 was partially restored. These effects of L. brevis 47 f strain were more pronounced or similar to those of the reference compound Rebamipid, a quinoline derivative known to protect the gut from drug-induced toxicity. Thus, the new lactobacilli strain attenuates the severity of 5 FU-induced enteropathy.

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Section: Discussionmentioning

confidence: 74%

“…Two strains, Lactobacillus plantarum 90 sk and L. brevis 47 f, demonstrated the most robust antioxidant properties. The formulation based on L. plantarum 90 sk showed an anti-depressant activity [ 33 ]. The cell-free supernatant of L. brevis 47 f reduced the oxidizing activity of paraquat and hydrogen peroxide.…”

Section: Introductionmentioning

confidence: 99%

The Lactobacillus brevis 47 f Strain Protects the Murine Intestine from Enteropathy Induced by 5-Fluorouracil

et al. 2020

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“…Overall, among the identified bifidobacterial species sharing the GAD/GABA antiporter locus, B. adolescentis , B. angulatum and B. dentium are of human origins, while the other five taxa are usually associated with the gut of other mammals, such as monkeys and bovines 17 – 19 . Between the above listed taxa of human origins, members of the B. adolescentis species are the most scrutinized for both genomic and proved production of GABA 20 – 22 . Intriguingly, the high level of prevalence of GAD/GABA antiporter locus within the 50 B. adolescentis genomes analyzed (94%) (Table S3 ), coupled with the fact that such bifidobacterial species are occurring in the human gut 20 , 23 , suggests that this bifidobacterial taxon might represent a model GABA producer.…”

Section: Resultsmentioning

confidence: 99%

Bifidobacterium adolescentis as a key member of the human gut microbiota in the production of GABA

Duranti

Ruíz

Lugli

et al. 2020

Sci Rep

156

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Gamma aminobutyric acid (GABA) is the principal inhibitory neurotransmitter playing a key role in anxiety and depression disorders in mammals. Recent studies revealed that members of the gut microbiota are able to produce GABA modulating the gut-brain axis response. Among members of the human gut microbiota, bifidobacteria are well known to establish many metabolic and physiologic interactions with the host. In this study, we performed genome analyses of more than 1,000 bifidobacterial strains publicly available revealing that Bifidobacterium adolescentis taxon might represent a model GABA producer in human gastrointestinal tract. Moreover, the in silico screening of human/animal metagenomic datasets showed an intriguing association/correlation between B. adolescentis load and mental disorders such as depression and anxiety. Interestingly, in vitro screening of 82 B. adolescentis strains allowed identifying two high GABA producers, i.e. B. adolescentis PRL2019 and B. adolescentis HD17T2H, which were employed in an in vivo trial in rats. Feeding Groningen rats with a supplementation of B. adolescentis strains, confirmed the ability of these microorganisms to stimulate the in vivo production of GABA highlighting their potential implication in gut-brain axis interactions. Gamma-Aminobutyric acid (GABA) is a non-protein amino acid that is widely distributed in plants, animals and microorganisms 1,2. GABA is synthetized by a pyridocal-5′-phosphate (PLP)-dependent glutamate decarboxylase (GAD) enzyme by irreversible α-decarboxylation of l-glutamate and consummation of one cytoplasmic proton 1,2. GABA has several well-known physiological and psychological functions. Different studies highlighted that it is predominantly present in the brain where it acts as a major inhibitory neurotransmitter in the mammalian central nervous system (CNS) 1,2. Specifically, dysfunctions in GABA metabolism are involved in anxiety and depression 3-5. Furthermore, it is involved in the regulation of blood pressure and heart rate and plays a role in the perception of pain and anxiety 5,6. Other potential health benefits of GABA are control of growth hormone secretion, protection against glycerol-induced acute renal failure in rats and anti-proliferative activity 7. Recently, the term "psychobiotic" has been introduced to designate live bacterial strains, including lactobacilli and bifidobacteria, which are able to influence the CNS function 8. There are several compounds produced by these bacteria, such as proteins, peptides and components of cell wall that are potential mediators between bacteria and their hosts. Neurotransmitters, such as GABA, represent an example of neuroactive molecules

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“…Probiotic strains are able to produce molecules that can influence the functioning of the central nervous system (CNS), behavior, and nociception [ 48 ]. GABA is a major neurotransmitter of the CNS, inhibiting the action potentials of neurons.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Probiotic Properties and the Capacity to Form Biofilms of Various Lactobacillus Strains

et al. 2020

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Over the last 20 years, Lactobacillus species inhabiting the gastrointestinal tract (GIT) have received much attention, and their health-promoting properties are now well-described. Probiotic effects cannot be generalized, and their uses cover a wide range of applications. It is thus important to proceed to an accurate selection and evaluation of probiotic candidates. We evaluate the probiotic potential of six strains of Lactobacillus in different in vitro models representing critical factors of either survival, efficacy, or both. We characterized the strains for their ability to (i) modulate intestinal permeability using transepithelial electrical resistance (TEER), (ii) form biofilms and resist stressful conditions, and (iii) produce beneficial host and/or bacteria metabolites. Our data reveal the specificity of Lactobacillus strains to modulate intestinal permeability depending on the cell type. The six isolates were able to form spatially organized biofilms, and we provide evidence that the biofilm form is beneficial in a strongly acidic environment. Finally, we demonstrated the ability of the strains to produce γ-aminobutyric acid (GABA) that is involved in the gut-brain axis and beneficial enzymes that promote the bacterial tolerance to bile salts. Overall, our study highlights the specific properties of Lactobacillus strains and their possible applications as biofilms.

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A Multi-strain Potential Probiotic Formulation of GABA-Producing Lactobacillus plantarum 90sk and Bifidobacterium adolescentis 150 with Antidepressant Effects

Cited by 82 publications

References 41 publications

The Lactobacillus brevis 47 f Strain Protects the Murine Intestine from Enteropathy Induced by 5-Fluorouracil

The Lactobacillus brevis 47 f Strain Protects the Murine Intestine from Enteropathy Induced by 5-Fluorouracil

Bifidobacterium adolescentis as a key member of the human gut microbiota in the production of GABA

Evaluation of the Probiotic Properties and the Capacity to Form Biofilms of Various Lactobacillus Strains

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