A multi-pumping flow analysis system for β-galactosidase activity assays

Skoczek, Justyna; Pokrzywnicka, Marta; Kubacka, Olga; Koncki, Robert

doi:10.1016/j.foodchem.2019.04.045

Cited by 7 publications

(2 citation statements)

References 28 publications

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“…The presented method demonstrated the possibility to be a good tool for available and cost-effective point-of-need screening fluoroquinolones. In 2019, a multipumping system was designed as a new assay for β-galactosidase activity 30 based on the determination of p-nitrophenol formed in the reaction of enzyme-catalyzed hydrolysis of p-nitrophenylgalactopyranosides. The method provided a high sample flowthroughput.…”

Section: Application Of Flow-based System For Quality Control and Foo...mentioning

confidence: 99%

Flow-based System: A Highly Efficient Tool Speeds Up Data Production and Improves Analytical Performance

et al. 2020

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In this review, we cite references from the period between 2015 and 2020 related to the use of a flow-based system as a tool to obtain a modern analytical system for speeding up data production and improving performance. Based on a great deal of concepts for automatic systems, there are several research groups introduced in the development of flow-based systems to increase sample throughput while retaining the reproducibility and repeatability as well as to propose new platforms of flow-based systems, such as microfluidic chip and paper-based devices. Additionally, to apply a developed system for on-site analysis is one of the key features for development. We believe that this review will be very interested and useful for readers because of its impact on developing novel analytical systems. The content of the review is categorized following their applications including quality control and food safety, clinical diagnostics, environmental monitoring and miscellaneous.

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Section: Application Of Flow-based System For Quality Control and Foo...mentioning

confidence: 99%

Flow-based System: A Highly Efficient Tool Speeds Up Data Production and Improves Analytical Performance

et al. 2020

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“…Such conditions are especially important in the case of enzyme activity assays. Therefore, several flow analysis systems designed to detect various hydrolases, such as alkaline phosphatase [ 9 , 10 ], acid phosphatase [ 10 ], α-amylase [ 11 ], urease [ 12 ] cutinase [ 13 ], and β-galactosidase [ 14 ], have been developed. Flow analysis systems for the assaying of the activity of oxidoreductases (such as catalase [ 15 ], peroxidase [ 16 ], and ceruloplasmin [ 17 ]) are less often reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

Multicommutated Flow Analysis System for Determination of Horseradish Peroxidase and Its Inhibitors

2021

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A fully mechanized multicommutated flow analysis (MCFA) system dedicated to determining horseradish peroxidase (HRP) activity was developed. Detection was conducted using a flow-through optoelectronic detector-constructed of paired LEDs operating according to the paired emitter-detector diode (PEDD) principle. The PEDD-MCFA system is dedicated to monitoring the enzyme-catalyzed oxidation of p-phenylenediamine (pPD) by a hydrogen peroxide. Under optimized conditions, the presented bioanalytical system was characterized by a linear response range (33.47–200 U/L) with a detection limit at 10.54 U/L HRP activity and 1.66 mV·L/U sensitivity, relatively high throughput (12 signals recordings per hour), and acceptable precision (RSD below 6%). Additionally, the utility of the developed PEDD-MCFA system for the determination of HRP inhibitors allowing the detection of selected thiols at micromolar levels, is demonstrated. The practical utility of the flow system was illustrated by the analysis of some dietary supplements containing L-cysteine, N-acetylcysteine, and L-glutathione.

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Flow-based food analytical methods

Economou

2021

Innovative Food Analysis

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A multi-pumping flow analysis system for β-galactosidase activity assays

Cited by 7 publications

References 28 publications

Flow-based System: A Highly Efficient Tool Speeds Up Data Production and Improves Analytical Performance

Flow-based System: A Highly Efficient Tool Speeds Up Data Production and Improves Analytical Performance

Multicommutated Flow Analysis System for Determination of Horseradish Peroxidase and Its Inhibitors

Flow-based food analytical methods

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