A multi-property fluorescent probe for the investigation of polymer dynamics near the glass transition

Siekierzycka, Joanna R.; Hippius, Catharina; Würthner, Frank; Williams, René M.; Brouwer, Albert M.

doi:10.2478/s11532-014-0544-0

Cited by 3 publications

(5 citation statements)

References 71 publications

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“…In addition to the different kinetics, a significant fraction of molecules did not switch off at all in the higher T g polymers (see the Supporting Information). This points towards a heterogeneous distribution of free volume, as previously discussed for single‐molecule rotation measurements as well as for polymers with different T g values through single‐molecule photoinduced electron‐transfer studies …”

Section: Figuresupporting

confidence: 64%

“…This points towards a heterogeneous distribution of free volume, as previously discussed for single-molecule rotation measurements [31] as well as for polymers with different T g values through single-molecule photoinduced electron-transfer studies. [32,33] Besides the photoswitching kinetics, the photostability of the probes determines their suitability for super-resolution fluorescence microscopy techniques. As shown in Figure 3, our photoswitch can perform many on-off cycles before photobleaching.…”

mentioning

confidence: 99%

“…This points towards ah eterogeneous distribution of free volume, as previously discussed for single-molecule rotation measure-ments [31] as well as for polymers with different T g values through single-molecule photoinduced electron-transfer studies. [32,33] Besides the photoswitching kinetics,the photostability of the probes determines their suitability for super-resolution fluorescence microscopy techniques.A ss hown in Figure 3, our photoswitch can perform many on-off cycles before photobleaching.T his might be of minor importance for probing af ixed structure where one localization event per fluorophore followed by irreversible photodamage is sufficient. Forprobing the dynamics,however,aquickly decreasing number of activatable switches is not desirable.H ere, photoswitches with high cycle numbers are of enormous advantage.W ec ould switch most of our photoswitches more than 100 times before irreversible photodamage occurred.…”

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confidence: 99%

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Nanoscopic Visualization of Soft Matter Using Fluorescent Diarylethene Photoswitches

et al. 2016

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The in situ imaging of soft matter is of paramount importance for a detailed understanding of functionality on the nanoscopic scale. Although super-resolution fluorescence microscopy methods with their unprecedented imaging capabilities have revolutionized research in the life sciences, this potential has been far less exploited in materials science. One of the main obstacles for a more universal application of superresolved fluorescence microscopy methods is the limitation of readily available suitable dyes to overcome the diffraction limit. Here, we report a novel diarylethene-based photoswitch with a highly fluorescent closed and a nonfluorescent open form. Its photophysical properties, switching behavior, and high photostability make the dye an ideal candidate for photoactivation localization microscopy (PALM). It is capable of resolving apolar structures with an accuracy far beyond the diffraction limit of optical light in cylindrical micelles formed by amphiphilic block copolymers.The nanoscopic structure of soft-matter materials determines their properties. [1] Therefore, methods to directly visualize structures in the nanometer range are of paramount importance for the ongoing evolution of novel materials with specialized and adaptive properties for sophisticated applications. Scanning probe microscopy techniques give access to the nanometer range and determine surface properties such as topology and softness, [2,3] while modern electron microscopy methods, such as scanning electron microscopy (SEM) [4,5] and transmission electron microscopy (TEM), [6][7][8] can yield structural information even in the subnanometer range when there is sufficient electron density contrast. Despite the success of these methods, they are technically demanding and time-consuming. Furthermore, many softmatter samples possess poor electron contrast, and require non-invasive in situ imaging below the surface as well as the possibility to directly study dynamics. In recent years, superresolved fluorescence microscopy has revolutionized optical imaging, [9][10][11][12][13][14] by utilizing the photophysical or photochemical switching of fluorescent dyes in a sophisticated manner in combination with modern optics. So far, the life sciences have benefited, in particular, from the new possibilities of resolving structures well beyond the diffraction limit of light. Only a few examples of the application of super-resolution microscopy to materials science have been reported, [15][16][17][18] since concepts that require, for example, the addition of (polar) switching buffers often fail for these systems. Therefore, the main bottleneck for more universal applications of super-resolution imaging are switchable dyes with suitable (photo-)physical and chemical properties, such as high photostability, adjustable switching rates, minimum interaction with the environment to be probed, and simple design, with the possibility of multiple and straightforward derivatization for the specific labeling of structures or compartments. [19] ...

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Section: Figuresupporting

confidence: 64%

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Nanoscopic Visualization of Soft Matter Using Fluorescent Diarylethene Photoswitches

et al. 2016

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“…Especially when labeling dense polymer phases, the photoswitching kinetics can be significantly influenced. One important parameter describing the polymer density and thus the free volume available for the photoswitch for its cyclization or cycloreversion is its glass transition temperature T g as has been demonstrated, e.g., by studies on photoinduced electron transfer . Irie and co‐workers describe the dependency of the DAE switching kinetics on the environment, and we previously observed a reduced switching behavior in highly viscous media as well .…”

Section: Resultsmentioning

confidence: 99%

Fluorescent Diarylethene Photoswitches—A Universal Tool for Super‐Resolution Microscopy in Nanostructured Materials

Nevskyi

Sysoiev

Dreier

et al. 2018

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Super-resolution fluorescence microscopy allows for unprecedented in situ visualization of biological structures, but its application to materials science has so far been comparatively limited. One of the main reasons is the lack of powerful dyes that allow for labeling and photoswitching in materials science systems. In this study it is shown that appropriate substitution of diarylethenes bearing a fluorescent closed and dark open form paves the way for imaging nanostructured materials with three of the most popular super-resolution fluorescence microscopy methods that are based on different concepts to achieve imaging beyond the diffraction limit of light. The key to obtain optimal resolution lies in a proper control over the photochemistry of the photoswitches and its adaption to the system to be imaged. It is hoped that the present work will provide researchers with a guide to choose the best photoswitch derivative for super-resolution microscopy in materials science, just like the correct choice of a Swiss Army Knife's tool is essential to fulfill a given task.

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“…Zusätzlich zur unterschiedlichen Kinetik konnte ein signifikanter Anteil an Molekülen in Polymeren mit hohem T g nicht ausgeschalten werden (siehe Hintergrundinformationen). Dies deutet auf eine heterogene Verteilung von freiem Volumen hin, wie es bereits über Messungen der Einzelmolekülrotation, und, für Polymere mit unterschiedlichen Glasübergangstemperaturen, mittels photoinduziertem Elektronentransfer auf Einzelmolekülebene beobachtet wurde …”

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Nanoskopische Bildgebung weicher Materie mittels fluoreszierender Diarylethen‐Photoschalter

et al. 2016

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Die In-situ-Bildgebung weicher Materie ist grundlegend füre in tieferes Verständnis von Funktionalitäta uf nanoskopischer Ebene.W ährend superhochauflçsende Fluoreszenzmikroskopiemethoden mit ihren bis dahin unerreichten Mçglichkeiten der Bildgebung die Lebenswissenschaften revolutionierten, wurde dieses Potenzial in den Materialwissenschaften bisher kaum ausgeschçpft. Dabei stellt die begrenzte Verfügbarkeit an geeigneten Farbstoffen zur Überwindung des Beugungslimits eines der wesentlichen Hindernisse füre inen breiteren Einsatz superhochauflçsender Mikroskopieverfahren dar.Hier berichtenwir über einen neuartigen Diarylethenbasierten Photoschalter mit einer stark fluoreszierenden geschlossenen und einer nicht-fluoreszierenden offenen Form. Seine photophysikalischen Eigenschaften, sein Schaltverhalten und seine hohe Photostabilitätm achen den Farbstoff zum idealen Kandidaten fürp hotoaktivierte Lokalisationsmikroskopie (PALM). Anhand von aus amphiphilen Blockkopolymeren aufgebauten zylindrischen Mizellenb elegen wir,w ie damit apolare Strukturen mit einer Auflçsung jenseits des Beugungslimits optischen Lichts abgebildet werden kçnnen.

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A multi-property fluorescent probe for the investigation of polymer dynamics near the glass transition

Cited by 3 publications

References 71 publications

Nanoscopic Visualization of Soft Matter Using Fluorescent Diarylethene Photoswitches

Nanoscopic Visualization of Soft Matter Using Fluorescent Diarylethene Photoswitches

Fluorescent Diarylethene Photoswitches—A Universal Tool for Super‐Resolution Microscopy in Nanostructured Materials

Nanoskopische Bildgebung weicher Materie mittels fluoreszierender Diarylethen‐Photoschalter

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