A multi-colorimetric immunosensor for visual detection of ochratoxin A by mimetic enzyme etching of gold nanobipyramids

Zhu, Hongshuai; Liu, Chuanhe; Liu, Xinxin; Zhu, Quan; Liu, Weipeng; Liu, Yingju

doi:10.1007/s00604-020-04699-5

Cited by 21 publications

(12 citation statements)

References 31 publications

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“…The optimal pH was first investigated since pH had a significant effect on the activity of mimetic peroxidase. The activity of Cu 2 O-based peroxidase was higher under acidic conditions and weaker under alkaline conditions. , Therefore, HAc-NaAc solutions with different pHs (3–12) were prepared and used as the diluent of H 2 O 2 for the further catalytic reaction with 4-CN. As shown in Figure A,B, τ showed the maximum value when the pH was 3 and 4 and gradually decreased as the pH increased.…”

Section: Resultsmentioning

confidence: 99%

“…The synthesis of Cu 2 O was based on our previous work. 25 First, 0.171 g of CuCl 2 • 2H 2 O was added into 100 mL of PVP aqueous solution (0.333 wt %), then 10 mL of NaOH (2 M) was introduced, and the solution was stirred at 55 °C for 0.5 h, during which the color of the solution changed from cyan to reddish brown. Afterward, 10 mL of ascorbic acid (AA, 0.6 M) was added, and the reaction was conducted at 55 °C for 1.5 h. The product was collected by centrifugation (8000 rpm, 10 min), washed with water and alcohol several times, and finally dried in vacuum.…”

Section: Methodsmentioning

confidence: 99%

“…The synthesis of Cu 2 O was based on our previous work . First, 0.171 g of CuCl 2 ·2H 2 O was added into 100 mL of PVP aqueous solution (0.333 wt %), then 10 mL of NaOH (2 M) was introduced, and the solution was stirred at 55 °C for 0.5 h, during which the color of the solution changed from cyan to reddish brown.…”

Section: Methodsmentioning

confidence: 99%

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Handheld Platform for Sensitive Rosiglitazone Detection: Immunosensor Based on a Time-Based Readout Device

et al. 2022

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The detection of rosiglitazone (RSG) in food is of great importance since the excessive intake of RSG could cause adverse effects on the human body. Although liquid chromatography–mass spectrometry and gas chromatography–mass spectrometry are the preliminary methods for the detection of hazardous materials in food, they are not suitable for point-of-care or on-site detection. Herein, a time-based readout (TBR) device with an application software (APP) controlled by a smart phone was developed for the sensitive and selective immunoassay of RSG. The homemade TBR device was based on a two-electrode system, where the immune molecule-modified glassy carbon electrode was used as the bioanode, and Prussian blue-modified FTO was used as the cathode. By using Au-modified octahedral Cu2O with high catalytic activity as mimetic peroxidase, an insulating layer was generated on the cathode by catalyzing 4-chloro-1-naphthol (4-CN) into benzo-4-chlorohexadienone (B4Q). The time to reach a fixed potential varied indirectly with the concentrations of RSG and was recognized by the APP, while the electrochromic property on the cathode was also correspondingly changed. Under optimum conditions, both the square root of the time and the chroma value of the electrochromism exhibited linear responses for the detection of RSG ranging from 5 × 10–10 to 5 × 10–7 g/L, while the limits of detection were 8.2 × 10–11 and 1.3 × 10–10 g/L, respectively. With easy operation and portability, this TBR device showed a promising application for point-of-care monitoring of hazardous materials in food or the environment.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Handheld Platform for Sensitive Rosiglitazone Detection: Immunosensor Based on a Time-Based Readout Device

et al. 2022

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“…Significantly, the immunoassay could successfully decrease the occurrence of false positives and false negatives during the detection of AFB 1 (Figure 3B). Furthermore, Zhu et al [101] developed a competitive ELISA that was constructed to sensitively monitor OTA in millet samples through octahedral Cu 2 O nanoparticles etching of Au nanobipyramids. Peroxidase-mimicking activity of Cu 2 O could oxidize TMB in the presence of H 2 O 2 , and the yellow product TMB 2+ could etch the Au nanobipyramids, triggering a significant longitudinal peak blue shift of local surface plasmon resonance.…”

Section: Nanozyme For Signal Amplificationmentioning

confidence: 99%

Advances in Visual Immunoassays for Sensitive Detection of Mycotoxins in Food—A Review

Liang

2021

The 1st International Electronic Conference on Chemical Sensors and Analytical Chemistry

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Mycotoxins are the toxic secondary metabolites naturally produced by fungi; their contamination in agricultural products and food severely threatens food safety and public health worldwide. The reliable, efficient, and sensitive quantification of mycotoxins in food has become increasingly challenging to tackle due to the complexity of food matrices and their low level. Visual detection has emerged as a popular trend toward miniaturization and simplification of mycotoxins assays yet is constrained with their limited sensitivity. This review mainly focuses on the various sensitive visual immunoassays for signal amplified detection of mycotoxins. These signal amplified immunoassays for the improved sensitivity of mycotoxins detection in food through nanomaterials for encapsulation enzyme, enzyme-mediated nanomaterials as the amplified signal readout, and nanozyme. Furthermore, the underlying principle and the advantages of visual immunoassays for mycotoxins have been proposed. And the challenges and perspectives have been proposed to develop improved efficient visual immunoassays for mycotoxins in food.

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“…25 For example, Lin et al proposed a multicolor biosensor for neuron specific enolase detection based on the peroxidase activity of Cu 2+ -modified carbon nitride nanosheets and etching of gold nanobipyramids (AuNBPs); 26 Zhu et al reported a multicolor biosensor for ochratoxin A detection based on the peroxidase activity of Cu 2 O octahedra. 27 Much effort still needs to be paid to develop multicolor biosensors based on mimic enzymes.…”

Section: Introductionmentioning

confidence: 99%

A multicolor biosensor for alkaline phosphatase activity detection based on the peroxidase activity of copper nanoclusters and etching of gold nanorods

Luo

Lin

Cai

et al. 2022

Analyst

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A multi-colorimetric immunosensor for visual detection of ochratoxin A by mimetic enzyme etching of gold nanobipyramids

Cited by 21 publications

References 31 publications

Handheld Platform for Sensitive Rosiglitazone Detection: Immunosensor Based on a Time-Based Readout Device

Handheld Platform for Sensitive Rosiglitazone Detection: Immunosensor Based on a Time-Based Readout Device

Advances in Visual Immunoassays for Sensitive Detection of Mycotoxins in Food—A Review

A multicolor biosensor for alkaline phosphatase activity detection based on the peroxidase activity of copper nanoclusters and etching of gold nanorods

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