A mu class glutathione S-transferase from Manila clam Ruditapes philippinarum (RpGSTμ): Cloning, mRNA expression, and conjugation assays

Bathige, S.D.N.K.; Umasuthan, Navaneethaiyer; Revathy, Kasthuri Saranya; Lee, Jehee; Kim, Seok-Ryel; Cho, Mi Young; Park, Myoung-Ae; Whang, Ilson

doi:10.1016/j.cbpc.2014.03.007

Cited by 11 publications

(8 citation statements)

References 39 publications

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“…In gills we found that GST mu transcription was highly up-regulated at 12 h post-exposure with the high exposure treatment causing a 6.2-fold increase. GST mu mRNA transcription induction in the gills was also reported by Bathige, et al [ 46 ] when R. philippinarum was challenged with LPS. These authors reported that the expression profile of GST mu revealed elevated levels in a time dependent manner.…”

Section: Resultssupporting

confidence: 77%

“…Individually, transcriptional patterns promoted by MC-LR exposure for the several GST isoforms varied not only within each organ but also between organs. The gills, as an external organ are in direct contact with the environment and possibly reflect short term exposure, whereas the hepatopancreas, being an internal organ where xenobiotics can accumulate, reflects long term exposure [ 40 , 46 ]. The different transcription patterns obtained for the tested GST isoforms in this study highlight the potential divergent physiological roles played by these isoenzymes during the detoxification of MC-LR.…”

Section: Resultsmentioning

confidence: 99%

“…These genes seemed to be more sensitive to MC-LR exposure in both organs. It has been suggested that mu and sigma class GSTs participate in cellular xenobiotic defenses and in the metabolism of products of oxidative stress [ 25 , 45 , 46 ] in different organisms.…”

Section: Resultsmentioning

confidence: 99%

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Transcriptional Responses of Glutathione Transferase Genes in Ruditapes philippinarum Exposed to Microcystin-LR

Reis

Carneiro

Machado

et al. 2015

IJMS

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Glutathione Transferases (GSTs) are phase II detoxification enzymes known to be involved in the molecular response against microcystins (MCs) induced toxicity. However, the individual role of the several GST isoforms in the MC detoxification process is still unknown. In this study, the time-dependent changes on gene expression of several GST isoforms (pi, mu, sigma 1, sigma 2) in parallel with enzymatic activity of total GST were investigated in gills and hepatopancreas of the bivalve Ruditapes philippinarum exposed to pure MC-LR (10 and 100 µg/L). No significant changes in GST enzyme activities were found on both organs. In contrast, MC-LR affected the transcriptional activities of these detoxification enzymes both in gills and hepatopancreas. GST transcriptional changes in gills promoted by MC-LR were characterized by an early (12 h) induction of mu and sigma 1 transcripts. On the other hand, the GST transcriptional changes in hepatopancreas were characterized by a later induction (48 h) of mu transcript, but also by an early inhibition (6 h) of the four transcripts. The different transcription patterns obtained for the tested GST isoforms in this study highlight the potential divergent physiological roles played by these isoenzymes during the detoxification of MC-LR.

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Section: Resultssupporting

confidence: 77%

Section: Resultsmentioning

confidence: 99%

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Transcriptional Responses of Glutathione Transferase Genes in Ruditapes philippinarum Exposed to Microcystin-LR

Reis

Carneiro

Machado

et al. 2015

IJMS

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“…To counteract the harmful effects of endogenous and exogenous compounds, such as drugs and mycotoxins, living organisms employ an array of enzymatic detoxification processes that have been classified into three distinct phases (Bathige et al 2014). Enzymes in phase I and II convert xenobiotics to less toxic water-soluble metabolites, which are later eliminated from the cell via phase III enzymes (Sheehan et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Based on the substrate specificity, antibody cross-reactivity and sensitivity to inhibitors, GSTs from various organisms have been identified as representing at least 15 different classes (Wang et al 2013). To date, many classes of GSTs have also been cloned and characterized in several aquatic species including the Pacific oyster Crassostrea gigas (Boutet et al 2004), rock shell Thais clavigera (Rhee et al 2008), disk abalone Haliotis discus discus (Wan et al 2008a), yellow catfish Pelteobagrus fulvidraco (Ku et al 2014) and Manila clam Ruditapes philippinarum (Bathige et al 2014). Regarding aquatic crustaceans, Contreras-Vergara et al (2004) reported the first Mu-class of GSTs in the white shrimp Litopenaeus vannamei .…”

Section: Introductionmentioning

confidence: 99%

Response of a Mu-class glutathione S-transferase from black tiger shrimp Penaeus monodon to aflatoxin B1 exposure

et al. 2016

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Glutathione S-transferases (GSTs) are a family of multifunctional phase II enzymes that are involved in the detoxification of exogenous and endogenous compounds. In this study, a full-length cDNA of Mu-class GST (PmMuGST) was isolated from the hepatopancreas of Penaeus monodon using rapid amplification of cDNA ends method. The full length cDNA of PmMuGST is 867 bp, contains an open read frame of 660 bp, and encodes a polypeptide of 219 amino acids with a molecular mass of 25.61 kDa and pI of 6.15. Sequence analysis indicated that the predicted protein sequence of PmMuGST was very similar to (86 %) that of Litopenaeus vannamei. A conserved domain of GST_N_Mu_like (PSSM: cd03075) and GST_C_family_superfamily_like (PSSM: cl02776) was indentified in PmMuGST. Real time quantitative RT-PCR analysis indicated that PmMuGST was present in all of the tested tissues. PmMuGST transcripts both in the hepatopancreas and in the muscle were significantly induced after 14 days of treatment with a low dosage of AFB1 (50 μg/kg) exposure and were significantly inhibited after 42 and 56 days of a high dosage of AFB1 (1000, 2500 μg/kg AFB1) exposure. Taken together, the Mu-class GST from P. monodon was inducible and was involved in the response to AFB1 exposure.

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Structure, Evolution and Functional Roles of Plant Glutathione Transferases

Chronopoulou

Ataya

Pouliou

et al. 2017

Glutathione in Plant Growth, Development, and Stress Tolerance

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A mu class glutathione S-transferase from Manila clam Ruditapes philippinarum (RpGSTμ): Cloning, mRNA expression, and conjugation assays

Cited by 11 publications

References 39 publications

Transcriptional Responses of Glutathione Transferase Genes in Ruditapes philippinarum Exposed to Microcystin-LR

Transcriptional Responses of Glutathione Transferase Genes in Ruditapes philippinarum Exposed to Microcystin-LR

Response of a Mu-class glutathione S-transferase from black tiger shrimp Penaeus monodon to aflatoxin B1 exposure

Structure, Evolution and Functional Roles of Plant Glutathione Transferases

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