A MRI-Based Toolbox for Neurosurgical Planning in Nonhuman Primates

Ojemann, William K. S.; Griggs, Devon J.; Ip, Zachary; Caballero, Olivya; Jahanian, Hesamoddin; Martínez-Conde, Susana; Macknik, Stephen L.; Yazdan-Shahmorad, Azadeh

doi:10.3791/61098

Cited by 10 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also demonstrated the ability to test electrical-stimulation-based interventions, as presented here and in a previous publication ( Griggs et al, 2021a ), in extension of prior post-lesion stimulation studies ( Nudo and Milliken, 1996 ; Nudo et al, 1996 ). While we demonstrated the use of this toolbox during acute lesioning, these same tools can be implemented for long-term studies in combination with a chronically implanted cranial window such as our previously published optogenetic interface ( Griggs et al, 2019 ; Khateeb et al, 2019a ; Ojemann et al, 2020 ; Yazdan-Shahmorad et al, 2015 , 2016 ), which exhibits the same spatial scale. In such studies, new lesions can be created or later enlarged without the need for additional surgical intervention, as would be required with the commonly employed electrocoagulation and cortical aspiration methods of focal cortical lesioning.…”

Section: Discussionmentioning

confidence: 99%

A versatile toolbox for studying cortical physiology in primates

Khateeb

Bloch

Zhou

et al. 2022

Cell Reports Methods

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

A versatile toolbox for studying cortical physiology in primates

Khateeb

Bloch

Zhou

et al. 2022

Cell Reports Methods

Self Cite

View full text Add to dashboard Cite

“…Taken together, the methods presented here serve as an accessible and inexpensive protocol to plan the optimized spread of infusions bench-side and validate the spread and accuracy in vivo, significantly reducing the number of unknowns that hinder confidence during circuit-manipulation experiments. Our presented methods contribute to a body of work supporting large-scale optogenetics in NHPs [ 8 , 9 , 16 , 17 , 18 , 19 , 22 , 23 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…Here, we have developed a quantitative bench-side CED model that provides the users with hands-on CED experience. Our bench-side model builds on our recent qualitative infusion modeling work [ 19 ], as well as our in vivo NHP data [ 8 , 9 ]. Bench-side CED models usually comprise dye infused into agar phantom, a clear gel with material properties similar to the brain [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Improving the Efficacy and Accessibility of Intracranial Viral Vector Delivery in Non-Human Primates

Griggs

Garcia

et al. 2022

Pharmaceutics

Self Cite

View full text Add to dashboard Cite

Non-human primates (NHPs) are precious resources for cutting-edge neuroscientific research, including large-scale viral vector-based experimentation such as optogenetics. We propose to improve surgical outcomes by enhancing the surgical preparation practices of convection-enhanced delivery (CED), which is an efficient viral vector infusion technique for large brains such as NHPs’. Here, we present both real-time and next-day MRI data of CED in the brains of ten NHPs, and we present a quantitative, inexpensive, and practical bench-side model of the in vivo CED data. Our bench-side model is composed of food coloring infused into a transparent agar phantom, and the spread of infusion is optically monitored over time. Our proposed method approximates CED infusions into the cortex, thalamus, medial temporal lobe, and caudate nucleus of NHPs, confirmed by MRI data acquired with either gadolinium-based or manganese-based contrast agents co-infused with optogenetic viral vectors. These methods and data serve to guide researchers and surgical team members in key surgical preparations for intracranial viral delivery using CED in NHPs, and thus improve expression targeting and efficacy and, as a result, reduce surgical risks.

show abstract

“…Our MMAD in combination with our previously presented chronic interfaces (Yazdan-Shahmorad et al ., 2015, 2016; Griggs et al ., 2019) equip us for a variety of research pursuits. Moving forward, we plan to capitalize on our previous optogenetic (Ledochowitsch et al ., 2015; Yazdan-Shahmorad et al ., 2015, 2016, 2018b, 2018a, 2018c; Khateeb et al ., 2019a; Ojemann et al ., 2020; Tremblay et al ., 2020) and imaging (Khateeb et al ., 2019b; Macknik et al ., 2019) experience as we pioneer the experimental spaces unveiled by the merging of large-scale ECoG and large-scale optical access to the NHP cortex. The synergistic power of these technologies poise us to build on our past work investigating neural plasticity (Yazdan-Shahmorad et al ., 2018a; Bloch et al ., 2019) as we study large-scale, chronic neural phenomena including neural disease, damage, and recovery.…”

Section: Discussionmentioning

confidence: 99%

Multi-modal artificial dura for simultaneous large-scale optical access and large-scale electrophysiology in non-human primate cortex

Griggs

Khateeb

Zhou

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Objective. Non-human primates (NHPs) are critical for development of translational neural technologies because of their neurological and neuroanatomical similarities to humans. Large-scale neural interfaces in NHPs with multiple modalities for stimulation and data collection poise us to unveil network-scale dynamics of both healthy and unhealthy neural systems. We aim to develop a large-scale multi-modal interface for NHPs for the purpose of studying large-scale neural phenomena including neural disease, damage, and recovery. Approach. We present a multi-modal artificial dura (MMAD) composed of flexible conductive traces printed into transparent medical grade polymer. Our MMAD provides simultaneous neurophysiological recordings and optical access to large areas of the cortex (~3 cm2) and is designed to mitigate photo-induced electrical artifacts. The MMAD is the centerpiece of the interfaces we have designed to support electrocorticographic recording and stimulation, cortical imaging, and optogenetic experiments, all at the large-scales afforded by the brains of NHPs. We performed electrical and optical experiments bench-side and in vivo with macaques to validate the utility of our MMAD. Main results. Using our MMAD we present large-scale electrocorticography from sensorimotor cortex of three macaques. Furthermore, we validated surface electrical stimulation in one of our animals. Our bench-side testing showed up to 90% reduction of photo-induced artifacts with our MMAD. The transparency of our MMAD was confirmed both via bench-side testing (87% transmittance) and via in vivo imaging of blood flow from the underlying microvasculature using optical coherence tomography angiography. Significance. Our results indicate that our MMAD supports large-scale electrocorticography, large-scale cortical imaging, and, by extension, large-scale optical stimulation. The MMAD prepares the way for both acute and long-term chronic experiments with complimentary data collection and stimulation modalities. When paired with the complex behaviors and cognitive abilities of NHPs, these assets prepare us to study large-scale neural phenomena including neural disease, damage, and recovery.

show abstract

A MRI-Based Toolbox for Neurosurgical Planning in Nonhuman Primates

Cited by 10 publications

References 0 publications

A versatile toolbox for studying cortical physiology in primates

A versatile toolbox for studying cortical physiology in primates

Improving the Efficacy and Accessibility of Intracranial Viral Vector Delivery in Non-Human Primates

Multi-modal artificial dura for simultaneous large-scale optical access and large-scale electrophysiology in non-human primate cortex

Contact Info

Product

Resources

About