A mouse urine phosphate assay for parathyroid hormone, with certain applications

Davies, Beryl M. A.; Gordon, A. H.; Mussett, Marjorie V.

doi:10.1113/jphysiol.1955.sp005394

Cited by 19 publications

(11 citation statements)

References 9 publications

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“…The procedure of Davies & Gordon (1955) was used, with some modifications. The defatted powder was dissolved in 80 % (v/v) acetic acid (12 ml./g.…”

Section: Preparation Of Acetic Acid Extractmentioning

confidence: 99%

“…Several brief reports, including the one of Handler, Cohn & Dratz (1954), have appeared on the unsuccessful attempts to use chromatography for isolation of the active principle. Because they felt that initial extraction with hydrochloric acid might be disadvantageous in later purification procedures, Davies & Gordon (1955) adopted acetic acid as the extracting agent. They showed that the active principle could be obtained by extraction with 80 % acetic acid, and further demonstrated that 50-65 % of the extracted hormonal activity would pass a cellophan membrane during ultrafiltration at pH 2-4.…”

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confidence: 99%

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The partial purification of parathyroid hormone by means of ultrafiltration and displacement chromatography

Rasmussen

Westall

1957

Biochemical Journal

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“…The procedure of Davies & Gordon (1955) was used, with some modifications. The defatted powder was dissolved in 80 % (v/v) acetic acid (12 ml./g.…”

Section: Preparation Of Acetic Acid Extractmentioning

confidence: 99%

mentioning

confidence: 99%

The partial purification of parathyroid hormone by means of ultrafiltration and displacement chromatography

Rasmussen

Westall

1957

Biochemical Journal

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“…Biological testing. The mouse urine-phosphate assay method for parathyroid hormone of Davies, Gordon & Mussett (1955) was used at each stage of the preparation.…”

Section: (Received 15 December 1954)mentioning

confidence: 99%

“…Assuming it to be some 20 units/ mg. (see p. 648 for the assumptions involved) we have a urine-phosphate/plasma-calcium activity ratio of about 2. Since this ratio was determined only once and the calcium activity measured only approximately, this result taken by itself is not important; however, it acquires interest when compared with the findings of Davies et al (1955), who obtained a urine-phosphate/plasma-calcium activity ratio of 2-6 for a preparation made according to L'Heureux et al (1947), and of Kenny, Vine & Munson (1954), who report a ratio of 3 6 for a 'crude hot acid extract ofbovine glands'.…”

Section: I955mentioning

confidence: 99%

Ultrafiltration of the parathyroid hormone

Davies

Gordon

1955

Biochemical Journal

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Effects of Phosphate Loading and Depletion on the Renal Excretion and Reabsorption of Inorganic Phosphate

Thompson¹,

Hiatt²

1957

J. Clin. Invest.

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A number of studies have indicated that parathyroid extract increases the renal excretion of phosphate (1)(2)(3)(4)(5)(6). This effect appears to result from a depression of the renal reabsorption of phosphate (6). Endogenous secretory activity of the parathyroid glands has been related to the extracellular concentration and/or the dietary intake of calcium and phosphate. Although dietary variations of these ions did not correlate consistently with TmP 8 in our initial investigations (7), other workers have demonstrated an influence of diet on phosphate reabsorption. A high phosphate diet led to a diminished P reabsorption in normal but not in parathyroidectomized rats (8). Studies in dogs (9) and in normal human subjects (8, 10) also indicated that the level of P intake influenced P reabsorption, but whether this effect was mediated by the parathyroid glands is not clear. In order to examine more rigorously the effects of a high P intake, buffered sodium phosphate solutions were administered intravenously daily for four to eight days to the normal and hypoparathyroid subjects previously described (7). Another phase of this study involved an inquiry into the mechanism by which aluminum gels produce a marked reduction in urinary P.Variations in plasma levels of Ca and P often occur together. An attempt was made to separate the effects of elevation of plasma P from the frequently seen simultaneous reduction in plasma Ca.Our data indicate that repeated daily intravenous administration of large quantities of phos- phate results in a reduction in renal P reabsorption. This effect does not appear to depend upon an associated reduction in plasma Ca. Whether this effect is mediated via the parathyroids cannot be conclusively stated. The results indicate that aluminum gels produce their effect primarily, and perhaps solely, by effecting a reduction in plasma P. METHODSThese studies were conducted on the same patients previously described, and the measurements of TmP were carried out by the method detailed in an earlier paper (7). In studies of the effects of intravenously administered phosphate, a buffered sodium phosphate solution, pH 7.4, was infused over a 4 to 6-hour period on four consecutive days. Five subjects received 75 mM of P each day; two subjects, C. H. and D. F., received 90 and 150 mM P per day, respectively. Two of the normal subjects, C. H. and E. P., received eight daily P infusions, and on the last four days they received, in addition, infusions of Ca. The Ca was administered as calcium gluconate, 15 mg. Ca per kg. of body weight in 1,000 ml. of 5 per cent glucose in water, over a fourhour period starting several hours after the completion of the phosphate infusion. Infusions of di-sodium ethylenediamine tetraacetic acid (EDTA) were given to the four normal subjects who received intravenous phosphate. Four gm. of EDTA was administered in 1,000 ml. of 5 per cent glucose in water over a 4 to 6-hour period daily for four consecutive days. TmP was measured prior to, and on the day following completion of t...

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A mouse urine phosphate assay for parathyroid hormone, with certain applications

Cited by 19 publications

References 9 publications

The partial purification of parathyroid hormone by means of ultrafiltration and displacement chromatography

The partial purification of parathyroid hormone by means of ultrafiltration and displacement chromatography

Ultrafiltration of the parathyroid hormone

Effects of Phosphate Loading and Depletion on the Renal Excretion and Reabsorption of Inorganic Phosphate

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