A Mouse Model of Zika Virus Sexual Transmission and Vaginal Viral Replication

Tang, William W.; Young, Matthew P.; Mamidi, Anila; Regla-Nava, José Ángel; Kim, Kenneth S.; Shresta, Sujan

doi:10.1016/j.celrep.2016.11.070

Cited by 138 publications

(147 citation statements)

References 36 publications

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“…To assess the clinical features, mice were checked each day and assigned a score between 1 and 7 as previously described (Tang et al, 2016). Weights were recorded, reported and compared to the initial weight obtained on the day of infection.…”

Section: Methodsmentioning

confidence: 99%

Mapping and Role of the CD8 + T Cell Response During Primary Zika Virus Infection in Mice

Ngono

Vizcarra

Tang

et al. 2017

Cell Host & Microbe

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215

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SUMMARY CD8+ T cells protect against and promote the pathogenesis of some flaviviruses, but their role in Zika virus (ZIKV) infection is unknown. We developed mice lacking the type I interferon receptor specifically in a subset of myeloid cells (LysMCre+IFNARfl/fl C57BL/6 (H-2b) mice) as a model for ZIKV infection and used it to evaluate CD8+ T cell responses to ZIKV. 26 and 15 CD8+ T cell-reactive peptides for ZIKV African (MR766) and Asian (FSS13025) lineage strains respectively were identified and validated. CD8+ T cells from infected mice were polyfunctional and mediated cytotoxicity. Adoptive transfer of ZIKV-immune CD8+ T cells reduced viral burdens, whereas their depletion led to higher tissue burdens and CD8−/− mice displayed higher mortality to ZIKV-infection. Collectively, these results demonstrate that CD8+ T cells protect against ZIKV infection. Further, this study provides an immunocompetent mouse model for investigating ZIKV-specific T cell responses.

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Section: Methodsmentioning

confidence: 99%

Mapping and Role of the CD8 + T Cell Response During Primary Zika Virus Infection in Mice

Ngono

Vizcarra

Tang

et al. 2017

Cell Host & Microbe

Self Cite

215

254

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“…For DENV challenge studies in AG129 mice, animals were passively transferred pooled vaccine immune sera or PBS one day prior to infection with ~10 5 FFU of DENV-2 S221. Animals were monitored for mortality and clinical score (1 = heathy; 2 = slightly ruffled fur; 3 = very ruffled fur; 4 = mild lethargy, decreased scurrying activity; 5 = very sick, slow to no movement; 6 = very sick, euthanize (in distress); 7 = deceased) as described previously (Tang et al, 2016).…”

Section: Experimental Model and Subject Detailsmentioning

confidence: 99%

Modified mRNA Vaccines Protect against Zika Virus Infection

Richner¹,

Himansu²,

Dowd³

et al. 2017

Cell

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SUMMARYThe emergence of ZIKV infection has prompted a global effort to develop safe and effective vaccines. We engineered a lipid nanoparticle (LNP) encapsulated modified mRNA vaccine encoding wild-type or variant ZIKV structural genes and tested immunogenicity and protection in mice. Two doses of modified mRNA LNPs encoding prM-E genes that produced virus-like particles resulted in high neutralizing antibody titers (~ 1/100,000) that protected against ZIKV infection and conferred sterilizing immunity. To offset a theoretical concern of ZIKV vaccines Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. eTOC A modified mRNA vaccine induces sterilizing immunity against Zika virus while avoiding the generation of cross-reactive antibodies that may enhance dengue infection. AUTHOR CONTRIBUTION HHS Public Access

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“…ZIKV also targets neuronal cell types 7,8 and cells of the reproductive tract, including spermatogonia, Sertoli and Leydig cells, and vaginal epithelium 2,9 . This extensive tropism is paralleled by the detection of ZIKV in multiple body fluids, including tears, saliva, semen, cervical mucus, and urine 2,10 .…”

Section: To the Editormentioning

confidence: 99%