A Mouse Homeo Box Gene Is Expressed in Spermatocytes and Embryos

Rubin, Michael R.; Toth, L E; Patel, Mayuri D.; D’Eustachio, Peter; Nguyen-Huu, M C

doi:10.1126/science.3726554

Cited by 119 publications

(56 citation statements)

References 38 publications

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“…12 point during the pachytene stage in the mutant when spermatogenesis was arrested, and carried out RT-PCR for Cdc25c as a late-pachytene marker of testicular RNA and for HoxA4 as a mid-pachytene marker. [18][19][20] HoxA4 expression was slightly decreased. In contrast, Cdc25c was not detected in the mutant testes (Fig.…”

Section: Resultsmentioning

confidence: 99%

Impairment of Pachytene Spermatogenesis inDmrt7Deficient Mice, Possibly Causing Meiotic Arrest

Date

Nozawa

Inoue

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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Section: Resultsmentioning

confidence: 99%

Impairment of Pachytene Spermatogenesis inDmrt7Deficient Mice, Possibly Causing Meiotic Arrest

Date

Nozawa

Inoue

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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“…Genes that are expressed before the pachytene spermatocyte stage, such as the DNA mismatch repair gene Mlh1 (Baker et al, 1996;Edelmann et al, 1996), and Dmc1, which is the mouse homolog of E. coli RecA (Habu et al, 1996), did not show significantly different expression patterns between the Mili +/-and Mili -/-genotypes. By contrast, the genes that are expressed in pachytene spermatocytes and at later stages, such as calmegin (Watanabe et al, 1994), Hoxa4 (Rubin et al, 1986), Cyclin A1 (Sweeney et al, 1996), and the gene encoding the cyclic AMP-responsive element modulator isoform Crem-τ (Foulkes et al, 1992), were not detectable in the Mili -/-testis. In addition, the expression of Miwi, which is present in midpachytene-stage spermatocytes, was not detected in the Mili -/-testes.…”

Section: Increased Apoptosis In Mili-null Micementioning

confidence: 99%

Mili, a mammalian member ofpiwifamily gene, is essential for spermatogenesis

et al. 2004

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The piwi family genes, which are defined by conserved PAZ and Piwi domains, play important roles in stem cell selfrenewal, RNA silencing, and translational regulation in various organisms. To reveal the function of the mammalian homolog of piwi, we produced and analyzed mice with targeted mutations in the Mili gene, which is one of three mouse homologs of piwi. Spermatogenesis in the MILI-null mice was blocked completely at the early prophase of the first meiosis, from the zygotene to early pachytene, and the mice were sterile. However, primordial germ cell development and female germ cell production were not disturbed. Furthermore, MILI bound to MVH, which is an essential factor during the early spermatocyte stage. The similarities in the phenotypes of the MILI-and MVH-deficient mice and in the physical binding properties of MILI and MVH indicate a functional association of these proteins in post-transcriptional regulation. These data indicate that MILI is essential for the differentiation of spermatocytes. Key words: Mili, Miwi, piwi, Mvh, Spermatogenesis SummaryMili, a mammalian member of piwi family gene, is essential for spermatogenesis

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“…The Scp1 (Sycp1 -Mouse Genome Informatics) and Scp3 (Sycp3 -Mouse Genome Informatics) genes, encoding synaptonemal complex proteins (Klink et al, 1997;Meuwissen et al, 1992), were also expressed at normal levels, demonstrating the presence of pachytene spermatocytes in mutant mice. However, the expression of calmegin (Watanabe et al, 1994) and Hox1.4 (Hoxa4 -Mouse Genome Informatics) (Rubin et al, 1986), which starts at the pachytene and persists through the spermatid stage, was significantly reduced in RanBPM -/-mice. Moreover, cyclin A1, the level of which has been reported to be upregulated in late pachytene spermatocytes and to peak in diplotene cells, was dramatically reduced (Sweeney et al, 1996).…”

Section: Research Article Role Of Ranbpm In Developmentmentioning

confidence: 99%

RanBPM is essential for mouse spermatogenesis and oogenesis

et al. 2011

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SUMMARYRanBPM is a recently identified scaffold protein that links and modulates interactions between cell surface receptors and their intracellular signaling pathways. RanBPM has been shown to interact with a variety of functionally unrelated proteins; however, its function remains unclear. Here, we show that RanBPM is essential for normal gonad development as both male and female RanBPM -/-mice are sterile. In the mutant testis there was a marked decrease in spermatogonia proliferation during postnatal development. Strikingly, the first wave of spermatogenesis was totally compromised, as seminiferous tubules of homozygous mutant animals were devoid of post-meiotic germ cells. We determined that spermatogenesis was arrested around the late pachytene-diplotene stages of prophase I; surprisingly, without any obvious defect in chromosome synapsis. Interestingly, RanBPM deletion led to a remarkably quick disappearance of all germ cell types at around one month of age, suggesting that spermatogonia stem cells are also affected by the mutation. Moreover, in chimeric mice generated with RanBPM -/-embryonic stem cells all mutant germ cells disappeared by 3 weeks of age suggesting that RanBPM is acting in a cell-autonomous way in germ cells. RanBPM homozygous mutant females displayed a premature ovarian failure due to a depletion of the germ cell pool at the end of prophase I, as in males. Taken together, our results highlight a crucial role for RanBPM in mammalian gametogenesis in both genders.

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A Mouse Homeo Box Gene Is Expressed in Spermatocytes and Embryos

Cited by 119 publications

References 38 publications

Impairment of Pachytene Spermatogenesis inDmrt7Deficient Mice, Possibly Causing Meiotic Arrest

Impairment of Pachytene Spermatogenesis inDmrt7Deficient Mice, Possibly Causing Meiotic Arrest

Mili, a mammalian member ofpiwifamily gene, is essential for spermatogenesis

RanBPM is essential for mouse spermatogenesis and oogenesis

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