A morphometric study of the intestinal mucosa of rats submitted to omentoenteropexy

Abstract

-Context -The omentoenteropexy technique was developed as an alternative method for intestinal neovascularization, due to the angiogenic properties of factors from the omentum. Objectives -This study investigated changes in intestinal villi heights and crypts depths due to surgical techniques: seromiotomy with and without omentoenteropexy. Method -Thirty rats were operated on, after being divided into three groups, namely GI, GII and GIII with 10 rats each. In the GI rats were submitted to omentoenteropexy; rats in GII were submitted only to a seromiotomy, and in the GIII only laparotomy. Sixty days after the first surgery, the animals were sacrificed and a segment of intestine was removed for histology using Masson's trichrome technique and morphometric study of intestinal mucosa.Results -The histological findings showed that seromiotomy with or without omentoenteropexy increased the length of intestinal villi when compared with GIII (only laparotomy) (analysis of variance: P = 0.0068; GI 38.88 ± 4.17; GII 39.41± 6.33; GIII 31.85 ± 5.56; GI = GII P>0.05; GII>GIII P<0.05; GI>GIII P<0.001). Conclusion -No differences were demonstrated in relation to crypt depths between the groups (P = 0.60). Ongoing studies are being set forth by our group to add more data on the role of omentopexy as a tool to promote neovascularization and intestinal mucosal growth.

Application of the MechPeff model to predict passive effective intestinal permeability in the different regions of the rodent small intestine and colon

“…Intestinal morphology parameters for villous height, villous width and inter‐villous spacing (distance between two adjacent villi) were either taken directly from published literature or digitally mapped from published electron microscope photomicrographs; data for villous height in the duodenum, jejunum and ileum were taken from references . The villous widths in the jejunum and the ileum were mapped manually from electron photomicrographs using GetData Graph Digitizer Version 2.22 (http://getdata-graph-digitizer.com) ; representative snapshots of the process for mapping villous widths and inter‐villous spacing are shown in Figure .…”

Enteric nervous system with chronic mesenteric ischemia: experimental study

“…В ряде случаев было отмечено спонтанное подпаивание сальника к стенке кишки. Как известно, оментопексия (подшивание сальника) широко используют в различных областях хирургии, учитывая высокую способность его тканей к ангиогенезу [22][23][24]. Неоваскуляризация стенки кишки через сальник может предотвратить некроз сегмента с нарушенным кровотоком, однако поддерживающего кровотока недостаточно для интрамуральных нервных клеток.…”