A morphometric study of neovascularization in colorectal carcinoma

Pavlopoulos, Petros M.; Konstantinidou, Anastasia; Agapitos, Emmanouil; Kavantzas, Nikolaos; Nikolopoulou, Polyxeni; Davaris, Panagiotis

doi:10.1002/(sici)1097-0142(19981115)83:10<2067::aid-cncr4>3.0.co;2-q

Cited by 53 publications

(70 citation statements)

References 17 publications

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“…Vessels with muscular walls were not counted; however there was no restriction regarding the size of the countable vessels, so as not to underestimate longitudinal sections or bifurcations of microvessels. 8 In addition to endothelial cells, megakaryocytes were also strongly positive for factor VIII. These cells were easily recognized by their characteristic size and morphology and served as internal controls to verify the adequacy of staining.…”

Section: Microvessel Counting and Image Analysismentioning

confidence: 99%

“…These points, at which a new branch emerged from a parent vessel, were recognized on the histological sections as Y-shaped, T-shaped or E-shaped. 8 The whole procedure took place without any knowledge of the patients' data. To confirm that the above counting technique was representative of bone marrow angiogenesis, we performed additional analysis in 10 randomly selected MDS cases.…”

Section: Figurementioning

confidence: 99%

“…Analogous observations have recently been made in colorectal carcinomas. 8 In accordance with Aguayo et al, 13 MVD remained unaffected among MDS subtypes, but the RA/RARS group displayed significantly larger vessel sections than RAEB resulting in higher minor axis length values. The higher MVD values recorded by Pruneri et al 17 are obviously attributable to the inclusion of RAEB-t cases (which would currently be designated AML 18 ), along with RAEB and CMML.…”

Section: Figurementioning

confidence: 99%

“…4 This contention was verified on multiple occasions for a variety of malignancies. [5][6][7][8][9] Correspondence: P Korkolopoulou, 73 Vassileos Pavlou str, GR-154 52 P Psychico-Athens, Greece; Fax: 30 Although the evidence in support of angiogenesis as an indicator of higher malignant potential in solid tumors is indisputable, the clinical significance of this phenomenon in most hematologic malignancies is at this time an unresolved issue. However, there is accumulating evidence indirectly implicating angiogenesis in the pathophysiology and course of some leukemias and lymphoproliferative disorders.…”

Section: Introductionmentioning

confidence: 99%

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Prognostic evaluation of the microvascular network in myelodysplastic syndromes

et al. 2001

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Considering the recently stated suggestion of neovascularization being implicated in myelodysplastic syndromes (MDS) pathogenesis, we evaluated multiple morphometric microvascular characteristics in MDS, in relation to clinicopathologic factors and prognosis. Trephines from 50 newly diagnosed MDS patients were immunostained for factor VIII and compared to those from 20 controls, 10 chronic myelomonocytic leukemia (CMML) and 12 acute myeloid leukemia (AML) patients. Quantitation of microvessel density (MVD), area, total vascular area (TVA), major and minor axis length, perimeter, compactness, shape factor, Feret diameter, and the number of branching vessels was performed by image analysis. Overall, the MDS group had significantly higher MVD, TVA, minor axis and shape factor values and significantly lower compactness than the control group. AML was characterized by increased vascularity compared to MDS and CMML, as well as by the presence of flattened microvessels (lower values of shape factor). Hypercellular MDS showed higher MVD. RA/RARS displayed larger caliber vessels than RAEB, which explains the favorable prognostic effect of increased size-related parameters on progression and/or survival. Moreover, decreased compactness and MVD were independent predictors of longer progression-free survival. It is concluded that angiogenesis is involved in the conversion of normal marrow to MDS and ultimately to AML and that disease progression within MDS is accompanied by qualitative alterations of the microvascular network. Furthermore, size-related parameters affect survival, while shape-related parameters and MVD are more influential with regard to progression-free survival. Leukemia (2001Leukemia ( ) 15, 1369Leukemia ( -1376

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Section: Microvessel Counting and Image Analysismentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prognostic evaluation of the microvascular network in myelodysplastic syndromes

et al. 2001

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“…Although most studies of advanced colorectal cancer have highlighted the prognostic value of microvessel counting, there have been some controversial data (3,17) which can be attributed to the use of methodology that vary considerably in the microvessel counting method, originally introduced by WEIDNER et al (32) and recently modified by VERMEULEN et al (30) . However, authors using this conventional microvessel counting method as BANNER et al (1) found higher MVD in colorectal carcinoma patients who had longer survival, but it was not statistically significant and PAVLOPOULOS et al (19) found prognostic significance in advanced colorectal carcinoma regarding only vascular ramifications and the total vascular area.…”

Section: Microvessel Count and Patient Survivalmentioning

confidence: 99%

Angiogenesis in advanced colorectal adenocarcinoma with special reference to tumoral invasion

Tarta

Teixeira

Tanaka

et al. 2002

Arq. Gastroenterol.

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-Background -Angiogenesis is a crucial step in tumor growth and progression. Its quantification by microvessel counting has a prognostic value in several types of malignancies and recently has been appraised in gastrointestinal tumors. Aim -To assess the prognostisc significance of microvessel quantification in colorectal carcinomas, studying its association with hematogenous metastases, survival and clinicopathological variables such as size, histologic differentiation and depth of tumoral invasion. Patients/Methods -Forty eight patients with colorectal adenocarcinoma were included in this study. Histologic sections of invasion tumoral margin (4 µm) were analyzed and endothellined microvessels were immunostained with monoclonal mouse Von Willebrand Factor (anti-FVIII). The microvessel count was performed from the identification of the area with increased microvessel density -hot spots -and results of the mean in five of these fields. Results -The cut-off microvessel count was 14 microvessels/0,785 mm 2 , which divided the sample into hypovascular and hypervascular groups. While 2/8 (25%) tumors with muscularis propria invasion were classified as hypervascular, 11/15 (73%) tumors with serosa or perivisceral fat were classified as hypervascular. However, a non-significant statistical association was found between the angiogenesis quantification, hematogenous metastases, survival and clinicopathological variables such as size and histologic differentiation of the tumor. Conclusions -The findings of significantly increase of microvessel count in conformity with tumoral invasion depth supports the hypothesis that tumor progression might be related to angiogenesis. Although angiogenesis is an important step in the tumoral growth and during the metastatization process, other factors can be implicated.

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Vegf, vegf-B, vegf-C and their receptors KDR, FLT-1 and FLT-4 during the neoplastic progression of human colonic mucosa

et al. 2000

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Because the crucial role of angiogenesis has been demonstrated in tumor growth and metastasis, the present study was undertaken to characterize the relative expression of vascular endothelial growth factors VEGF (vascular endothelial growth factor), VEGF‐B, VEGF‐C, and their receptors KDR (kinase insert domain‐containing receptor), FLT‐1 (fms‐like tyrosine kinase), and FLT‐4 in human colonic cancers, in relation to the Astler‐Coller pathological classification, and to prognosis. VEGF and VEGF‐B gene expression was quantified by Northern blot in 72 tumor samples matched with control tissues. VEGF gene expression was 1.4 times higher in adenocarcinomas than in control tissues (p = 0.02), but did not increase further between Astler‐Coller tumor stages A and D, and did not correlate with disease recurrence for patients at stages B2 or C. In adenomas, VEGF mRNA levels were not significantly different from those in the paired control colonic mucosa. The expression pattern of VEGF isoforms, mainly identified by RT‐PCR (reverse‐transcriptase‐coupled polymerase chain reaction) as VEGF121 and VEGF165 and to a lesser extent VEGF189, was comparable in tumor and control tissues. VEGF‐B mRNA levels were unchanged during the neoplastic progression of colonic mucosa. In contrast to KDR and FLT‐4, the expression of VEGF‐C and FLT‐1 genes increased in some pathological tissues. These results provide evidence that the early and sustained increase in VEGF transcripts and the expression of multiple angiogenic factors and receptors contribute to the development of colon cancer, and thus constitute a putative target for anti‐angiogenic drug therapy. Int. J. Cancer 86:174–181, 2000. © 2000 Wiley‐Liss, Inc.

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A morphometric study of neovascularization in colorectal carcinoma

Cited by 53 publications

References 17 publications

Prognostic evaluation of the microvascular network in myelodysplastic syndromes

Prognostic evaluation of the microvascular network in myelodysplastic syndromes

Angiogenesis in advanced colorectal adenocarcinoma with special reference to tumoral invasion

Vegf, vegf-B, vegf-C and their receptors KDR, FLT-1 and FLT-4 during the neoplastic progression of human colonic mucosa

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