A morphological study of the lymphocyte traffic in Peyer's patches after an in vivo antigenic stimulation

Regoli, Marì; Borghesi, Chiara; Bertelli, Eugenio; Nicoletti, Claudio

doi:10.1002/ar.1092390106

Cited by 35 publications

(20 citation statements)

References 17 publications

(19 reference statements)

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“…This structural heterogeneity, however, cannot be explained simply by the difference in the stage of M cell maturation (Spit et al 1989). In turn, there is a possibility that the excessive influx of immunocompetent cells into FAE might subsequently induce some subcellular changes in M cells (Regoli et al 1994). This is also suggested by the present observation that M cells in SPF-CV rats showed a relative increase in keratin filaments and electrondense spherical bodies, and extensive cytoplasmic interfoliation.…”

Section: supporting

confidence: 66%

“…In fact, the infiltration of immunocompetent cells into the FAE containing M cells was increased when the duration of maintenance under conventional conditions was protracted. Lymphocyte migration into M cells therefore seems to be an antigendependent event (Regoli et al 1994), and the immune cells in the FAE appear to be able to induce an increase in the number of M cells (Fo$ rster et al 1996) and alterations in the FAE (Borghesi et al 1996). This structural heterogeneity, however, cannot be explained simply by the difference in the stage of M cell maturation (Spit et al 1989).…”

Section: mentioning

confidence: 99%

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Ultrastructural study on the follicle‐associated epithelium of nasal‐associated lymphoid tissue in specific pathogen‐free (SPF) and conventional environment‐adapted (SPF‐CV) rats

et al. 2000

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Membranous (M) cells in follicle-associated epithelium (FAE) play an important role in the mucosal immunity through transport of a variety of foreign antigens to the underlying mucosa-associated lymphoid tissue (MALT). We aimed to investigate the ultrastructure of M cells in the FAE covering nasal-associated lymphoid tissue (NALT) both in specific pathogen-free (SPF) rats and in conventional environment-adapted (SPF-CV) rats aged 8-38 wk. In NALT of both SPF and SPF-CV rats, FAE included the nonciliated microvillous cell, which appears to be an analogue of M cell previously described in other MALT. In SPF rats, M cells increased in number only slightly with age, and they maintained morphological uniformity irrespective of age. In SPF-CV rats, M cells selectively increased in number resulting in prominent expansion of FAE surface area in parallel with the duration of maintenance in a conventional environment. In addition, M cells in SPF-CV rats showed heterogeneity in their surface morphology such as the length and number of microvilli and cell surface area and outline. In addition, the FAE was stratified by various subtypes of M cells, which were characterised by several subcellular alterations including the presence of many keratin filaments, homogeneous dark bodies and extensive cytoplasmic interfoliation with wide intercellular spaces filled with amorphous proteinaceous material. These characteristics of M cells in SPF-CV rat were intimately related with a preferential influx of immunocompetent cells into the FAE, which was not seen or was very rare in SPF rats irrespective of age. The results suggest the possibility that NALT may effectively carry out the mucosal immune response against antigenic stimuli of different magnitude through the unique dynamics of M cells which seem to be influenced by the infiltration of immunocompetent cells.

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Section: supporting

confidence: 66%

Section: mentioning

confidence: 99%

Ultrastructural study on the follicle‐associated epithelium of nasal‐associated lymphoid tissue in specific pathogen‐free (SPF) and conventional environment‐adapted (SPF‐CV) rats

et al. 2000

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“…In other studies, however, discontinuities appeared to contain different types of cells: some resembled migratory lymphocytes (Nair and Rossinsky 1984), while others were rounded cells with either a villous or a smooth surface (Maeda et al 1978). These differences in cell appearance may reflect deformation of the M cell cytoplasm produced by lymphocytes migrating to the gastrointestinal lumen (Regoli et al 1994). Such migration, however, appears to be largely antigen-dependent (Heatley and Bienenstock 1982;Regoli et al 1994).…”

Section: Discussionmentioning

confidence: 99%

The epithelium of canine palatine tonsils

Belz

Heath

1995

Anat Embryol

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The palatine tonsil is positioned to play a key role in protecting the body against ingested microorganisms. These microorganisms must traverse the tonsillar epithelium to initiate immune reactions, but no information is available from dogs on the structure of this epithelium. In this study, the morphology and ultrastructure of the epithelium of the palatine tonsil of dogs was examined using light and electron microscopy. The epithelium is of two types: reticular and non-reticular. Reticular epithelium, which is invaded by lymphoid cells, is located over the apices of nodules, separated indistinctly by islands of non-reticular squamous epithelium. The reticular epithelium contains M cells which are more abundant towards the periphery of lymphoid nodules. The apical membrane of the M cells forms folds from which microvilli extend into the lumen of the oropharynx. Abluminally, cytoplasmic processes enfold clusters of lymphocytes. Numerous desmosomes secure the marginal plasma membrane and lateral membranous interdigitations to adjacent epithelial cells, thereby maintaining the integrity of the tonsillar epithelium. Epithelial discontinuities, which are few, occupy similar positions to M cells and appear to contain migrating lymphocytes. A fenestrated basement membrane allows cell transport between the intraepithelial passageways of the reticular epithelium and the subepithelial lymphoid tissues.

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“…Although M cells have been studied for Makala/Nishikawa/Suzuki/Nagasawa ]. An immunological role for M-cell-associated lymphocytes is suggested by the observation that their numbers increase after application of a bacterial challenge [18,107]. The authors showed that in these circumstances, a large number of cells migrate into the intestinal lumen via M-cell-gated diapedesis.…”

Section: Role Of Intestinal Membranous Cellsmentioning

confidence: 99%

Immunology

et al. 2004

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It has been known for the past 85 years that mucosal responses can be stimulated by local presentation of antigen and that the gut immune system is capable of mounting both primary and secondary responses to potentially harmful antigens while avoiding the expression of damaging responses to harmless dietary proteins. How these types of responses are induced and regulated remains unclear. In the gut attention has for some time been focused on Peyer’s patches (PP) due to evidence that they play a vital role in the induction of humoral and cellular responses. Moreover, it has been established that MHC class II molecules are found in the gut mucosa on a variety of cell types outside PP, namely the lamina propria (LP). Fed antigens have also been detected in the LP and studies have shown that LP cells can stimulate allogeneic mixed lymphocyte responses and are capable of presenting soluble protein antigen to naïve T cells. This article reviews the present understanding of the possible roles of PP and LP in intestinal immunity in terms of induction, regulation, surveillance of immune responses and the antigen presenting cell types involved.

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A morphological study of the lymphocyte traffic in Peyer's patches after an in vivo antigenic stimulation

Cited by 35 publications

References 17 publications

Ultrastructural study on the follicle‐associated epithelium of nasal‐associated lymphoid tissue in specific pathogen‐free (SPF) and conventional environment‐adapted (SPF‐CV) rats

Ultrastructural study on the follicle‐associated epithelium of nasal‐associated lymphoid tissue in specific pathogen‐free (SPF) and conventional environment‐adapted (SPF‐CV) rats

The epithelium of canine palatine tonsils

Immunology

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