A morphological study of the influence of fluoride on rat molar teeth

Gray, Harriet

doi:10.1016/0003-9969(73)90121-0

Cited by 10 publications

(5 citation statements)

References 21 publications

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“…1). Using higher doses of fluoride, previous studies [40,41] have demonstrated a significant increase in the width of the enamel in the fluoride-treated rats, but this did not occur in this study ( Table 1).…”

Section: Discussioncontrasting

confidence: 81%

A microspectrophotometric analysis of the effect of fluoride on immature enamel matrix protein of rat molar teeth

Smid

Kruger

1980

Calcif Tissue Int

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Over a 24-h period, Wistar rats from 4 litters, 6 to 9 days old, were given five intraperitoneal injections of a solution of 0.9% sodium chloride containing sodium fluoride (3 mg F/kg body weight). Within-litter controls were used. All rats were killed by decapitation 2 h after the final injection and the rat heads, cut sagittally, were processed for protein histochemistry. The intensity of staining of the protein in the enamel matrix of the upper jaw molar tooth buds was quantified using the two-wavelength method of microphotometry. A significant increase in the intensity of staining of fluoride-treated tissues over controls was observed with the histochemical methods specific for arginine (P less than 0.01), tyrosine (P less than 0.05), and cysteine (P less than 0.05). Other histochemical methods specific for amino acid groups failed to show any significant difference between fluoride and non-fluoride-treated enamel matrix.

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Section: Discussioncontrasting

confidence: 81%

A microspectrophotometric analysis of the effect of fluoride on immature enamel matrix protein of rat molar teeth

Smid

Kruger

1980

Calcif Tissue Int

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“…Kruger [1962Kruger [ , 1964Kruger [ , 1966, however, found no change in mesiodistal mo lar widths, hut did find wider fissures. The results of Gray [1973] contrast with these as he found deeper fissures with no change in fissure width nor in molar width as rats consumed more fluoride. Therefore, there is a lack of consistency in the results of previous fluoride/enamel morphology studies.…”

contrasting

confidence: 48%

“…The fluoride levels were chosen based on recom mendations for proper growth and development in rats [National Research Council, 1978], The finding that the group which drank water with 50 ppm F" had significantly smaller pups was both unexpected and is in contrast to the results of Gray [1973] and Drinkard et al [1984] for the same concentration. Although the body size was decreased, the tooth size was not af fected.…”

Section: Discussionmentioning

confidence: 42%

“…One of the most remarkable find ings of the present study was the increase in fissure depth of rat mandibular molars in animals drinking water with higher fluoride concentrations than the control animals. The studies of Gray [1973] and Paynter and Grainger [1956] showed similar tendencies, though neither of their results were significant. In contrast, Krüger [1962Krüger [ , 1964Krüger [ , 1966 found signifi cantly shallower fissures in rats obtaining higher flu oride concentrations.…”

Section: Discussionmentioning

confidence: 54%

“…In con trast, Krüger [1962Krüger [ , 1964Krüger [ , 1966 found wider fissures. Paynter and Grainger [1956] and Gray [1973], how ever, found a tendency towards slightly narrower fis sure widths, though, again, these were not statistically significant results.…”

Section: Discussionmentioning

confidence: 89%

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Effect of Systemic Fluoride on Rat Molar Morphology

Creath¹,

Eick²,

Hicks³

1989

Caries Res

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Female Sprague-Dawley rats were divided into three groups. All three groups were given the same solid diet (with 1.6 ppm F). Group I was given deionized water, group II drank water with 5 ppm fluoride added, and group III had 50 ppm fluoride added. All females were mated with the same male. The pups were sacrificed at 28 days, and the mandibles were removed and cleaned. Three-dimensional representations of the occlusal surfaces of the 1st and 2nd mandibular molars were reproduced via computer-digitized images using stereophotogrammetry. Fissure depths and widths were measured, and ‘volumes’ of the fissures were computed. The fissure widths, depths, and volumes were compared statistically using unpaired t tests between groups (p < 0.05). There were significant differences in fissure depth between groups I and II and between groups I and III. For fissure volumes, significant differences were found between groups I and III. The width of the fissure and the overall tooth size were not significantly different between fluoride-treated groups. Standardization showed a reproducibility to within ± 40 mm with the stereophotogrammetric method.

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Regeneration of tooth development in vitro following sodium fluoride treatment

Kerley

Kollar

1977

Am. J. Anat.

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Mandibular incisors were dissected from the jaw of 15- and 16-day C57BL/10 mouse embryos and cultured on agar-solidified Eagle's basal medium supplemented with fetal calf serum, an antibiotic, and glutamine, The experimental medium was the same as the control except that fluoride was added such that the final concentrations ranged from 2.0-8.0 mM NaF. Control and experimental explants were recovered after two, four and six days of incubation and studied histologically. After two days of fluoride treatment (3.0 mM NaF), cellular degeneration was observed in the dental papilla mesenchyme while the enamel organ epithelium appeared more resistant. Prolonged treatment or treatment at higher concentrations resulted in destruction of the dental papilla. The enamel organ was still present but was abnormal and reduced. Older tooth germs were less affected overal when incubated at the same fluoride dosage and time of treatment. When explants subjected to limited exposure (2 days) to fluoride were placed on control medium, the suppressed tooth germs recovered. The recovery was enhanced by grafting untreated mesenchyme to the treated explants followed by incubation on control medium. The observations indicate that NaF can suppress the development of tooth germs in vitro and that recovery from the suppresion does occur. The more severe inhibition observed in the mesenchymal component when compared to the response of the epithelial component of the treated explants suggests that fluoride may alter the ultimate morphology of the tooth crown by disrupting the normal epithelial-mesenchymal interaction which occurs during early tooth development.

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A morphological study of the influence of fluoride on rat molar teeth

Cited by 10 publications

References 21 publications

A microspectrophotometric analysis of the effect of fluoride on immature enamel matrix protein of rat molar teeth

A microspectrophotometric analysis of the effect of fluoride on immature enamel matrix protein of rat molar teeth

Effect of Systemic Fluoride on Rat Molar Morphology

Regeneration of tooth development in vitro following sodium fluoride treatment

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