A morphological study of cells isolated from the perfused gill of dab and Atlantic salmon

Lin, S.-H.; Davidson, G.A.; Secombes, Chris J.; Ellis, A.E.

doi:10.1111/j.1095-8649.1998.tb01001.x

Cited by 31 publications

(16 citation statements)

References 18 publications

(26 reference statements)

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“…Macrophages, lymphocytes, neutrophils, and eosinophils (rodlet cells) were previously observed in the gill epithelium of tilapia (Wendelaar Bonga and Van der Meij, 1989;Lin et al, 1998) and other teleosts (Laurent, 1984;Pratap and Wendelaar Bonga, 1993). These cells are thought to be migratory, with non-granulated precursors originating in hematopoietic system and circulating, therefore, toward the tissues, where the last steps of cell maturation occur (Flañ o et al, 1996;Holland and Rowley, 1998).…”

Section: Discussionmentioning

confidence: 92%

“…The gill epithelium is differentiated into a multilayered filament epithelium in the interlamellar regions, and a continuous superficial monolayer of pavement cells (PVC) that covers the filament and the respiratory lamellae. Various cell types have been identified in the filament epithelium of teleosts, such as PVC, mucous cells (MC), mitochondria-rich cells (MRC), accessory cells, neuroepithelial/neuroendocrine cells (NEC), support cells (SC), basal cells, urea-excreting cells, and leukocytes (Laurent, 1984;Maina, 1991;Pratap and Wendelaar-Bonga, 1993;Lin et al, 1998;Monteiro et al, 2004).…”

Section: Introductionmentioning

confidence: 97%

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Fine structure of the branchial epithelium in the teleost Oreochromis niloticus

Monteiro

Oliveira

Fontaínhas‐Fernandes

et al. 2010

Journal of Morphology

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We have studied the gill epithelium of Oreochromis niloticus using transmission electron microscopy with the particular interested relationship between cell morphology and osmotic, immunoregulatory, or other non-regulatory functions of the gill. Pavement cells covered the filament epithelium and lamellae of gills, with filament pavement cells showing distinct features from lamellar pavement cells. The superficial layer of the filament epithelium was formed by osmoregulatory elements, the columnar mitochondria-rich, mucous and support cells, as well as by their precursors. Light mitochondria-rich cells were located next to lamellae. They exhibited an apical crypt with microvilli and horizontal small dense rod-like vesicles, sealed by tight junctions to pavement cells. Dark mitochondria-rich cells had long dense rod-like vesicles and a small apical opening sealed by tight junctions to pavement cells. The deep layer of the filament epithelium was formed by a network of undifferentiated cells, containing neuroepithelial and myoepithelial cells, macrophage and eosinophil-like cells and their precursors, as well as precursors of mucous cells. The lateral-basal surface was coated by myoepithelial cells and a basal lamina. The lamellar blood lacunae was lined by pillar cells and surrounded by a basal lamina and pericytes. The data presented here support the existence of two distinct types of pavement cells, mitochondria-rich cells, and mitochondria-rich cells precursors, a structural role for support cells, a common origin for pavement cells and support cells, a paracrine function for neuroepithelial cells in the superficial layer, and the control of the lamellar capillary base by endocrine and contractile cells. Data further suggest that the filament superficial layer is involved in gill osmoregulation, that may interact, through pale mitochondria-rich cells, with the deep layer and lamellae, whereas the deep layer, through immune and neuroendocrine systems, acts in the regeneration and defense of the tissue.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 97%

Fine structure of the branchial epithelium in the teleost Oreochromis niloticus

Monteiro

Oliveira

Fontaínhas‐Fernandes

et al. 2010

Journal of Morphology

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“…Leucocytes and plasma cells are also present in the skin and gills, and antigen can be taken up by these epithelia (Lobb, 1987;Iger & Wendelaar Bonga, 1994;Davidson et al, 1997;Lin et al, 1998;Moore et al, 1998). However, not much is known about antigen processing in the skin and gills, although immunocompetent cells are present (Rombout et al, 1993) and recent investigations have shown that MHC class II chain mRNA expression is high in gills of Atlantic salmon (Koppang et al, 1998b).…”

Section: Mucosa-associated Lymphoid Tissuementioning

confidence: 97%

The morphology of the immune system in teleost fishes

Press

Evensen

1999

Fish & Shellfish Immunology

528

269

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“…Although teleost mast cells are abundant around blood vessels and host -environment interfaces such as the skin, gills and alimentary tract, their function in defense is not clearly defined. Teleost mast cells store a number of mediators, such as phospholipids, alkaline phosphatase, aryl sulphatase B, 5-nucleotidase, acid phosphatase, peroxidase and lysozyme, which participate in bacterial inactivation and degradation (Ezeasor & Stoke 1980, Powell et al 1992, Sire & Vernier 1995, Sveinbjørnsson et al 1996, Lin et al 1998. The presence of piscidins in mast cells of diverse fish species suggests that they perform some type of critical defensive function.…”

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confidence: 99%

Evidence for widespread distribution of piscidin antimicrobial peptides in teleost fish

Silphaduang¹,

Colorni²,

Noga³

2006

Dis. Aquat. Org.

162

126

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Antimicrobial peptides (AMPs) are increasingly recognized as a critical component of the host's defense against infection. Several types of AMPs have been recently identified from mucosal tissues or immune cells of a number of teleosts. Among these are the piscidins, which are 22 residue, α-helical AMPs that were originally isolated from mast cells of hybrid striped bass Morone saxatilis male × Morone chrysops female. Using an antibody specific for the conserved N-terminal amino acid sequence of piscidin 1, we used immunohistochemistry to probe skin, gill, and gastrointestinal tract of 39 teleosts representing 7 different orders. Nine fish species were piscidin-positive, with all of these species being in the Perciformes, the largest and most evolutionarily advanced order of teleosts. Piscidin-positive cells were identified in species belonging to the families Moronidae, Serranidae, Sciaenidae, Siganidae and Belontidae. Immunopositive cells were usually most consistent with mast cells, although in some species, the granule appearance and tinctorial properties diverged somewhat from those of a typical piscine mast cell. In addition, rodlet cells were piscidin-positive in one member of the family Cichlidae; to our knowledge, it is the first time that a host-associated chemical biomarker has been identified in rodlet cells. Our data suggest that piscidins are present in many evolutionarily advanced teleosts. Piscidin-immunoreactive cells were most common at sites of pathogen entry, including the skin, gill and gastrointestinal tract. These results strongly suggest that piscidins are a widespread and important component of many fishes' defense against disease. KEY WORDS: Piscidins · Mast cells · Rodlet cells · Innate immunity · Pisces Resale or republication not permitted without written consent of the publisherDis Aquat Org 72: [241][242][243][244][245][246][247][248][249][250][251][252] 2006 tissues of hybrid striped bass; the parental stocks of hybrid striped bass (white bass Morone chrysops and striped bass M. saxatilis) are in the family Moronidae, and similar appearing cells were positive in members of the family Sciaenidae (spot Leistomus xanthurus and Atlantic croaker Micropogonias undulatus). This prompted us to hypothesize that piscidins are present in other teleosts. To answer this question, we immunochemically examined tissues from 7 different orders of teleosts. Presumptively healthy, subadult to adult, farm-raised fish, as well as wild-caught individuals, were examined in the present study. MATERIALS AND METHODSPeptide synthesis. Piscidin 1 (both the entire peptide and an 11-mer fragment) and Magainin 2 peptides were synthesized using Fmoc chemistry on a Rainin Symphony instrument that provides on-instrument cleavage of the peptide from the resin. After synthesis, peptides were purified via analytical reverse phase HPLC using a YMC C-18 column (4 × 50 mm, 3 µm particle size, 120 Å pore size support) using an acetonitrile gradient that was eluted at 1 ml min -1 where Buffer A was 0.05% trif...

show abstract

A morphological study of cells isolated from the perfused gill of dab and Atlantic salmon

Cited by 31 publications

References 18 publications

Fine structure of the branchial epithelium in the teleost Oreochromis niloticus

Fine structure of the branchial epithelium in the teleost Oreochromis niloticus

The morphology of the immune system in teleost fishes

Evidence for widespread distribution of piscidin antimicrobial peptides in teleost fish

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