A more sensitive assay discriminating galactosamine and glucosamine in mixtures

Wagner, William D.

doi:10.1016/0003-2697(79)90379-8

Cited by 216 publications

(94 citation statements)

References 6 publications

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“…In the differential assay for glucosamine and galactosamine, we used the modified procedure of Wagner. 31 Standards of 2 to 40 /ig/ml (glucosamine HCI and galactosamine HCI, Sigma Chemical Company, St. Louis, Missouri) were used in both assays.…”

Section: Methodsmentioning

confidence: 99%

Aortic glycosaminoglycans in genetically selected WC-2 pigeons with increased atherosclerosis susceptibility.

Nohlgren¹

1981

Arteriosclerosis

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A genetically selected line of White Carneau pigeons (WC-2) was studied In an attempt to relate changes in composition and content of aortic glycosamlnoglycan (GAG) to increased atherosclerosis susceptibility. The WC-2 pigeons were fed an atherogenlc diet for 3 months and, when compared to randomly bred White Carneau (RBWC) controls, they showed similar plasma cholesterol concentrations but significantly greater aortic atherosclerosis. In the WC-2 pigeons, 35% of the aortic surface was covered with plaque compared with only 12% in RBWC pigeons; WC-2 birds showed cholesterol contents of 3.3 mg/aorta/500 g body weight, while the RBWC birds had only 0.9 mg/aorta/500 g body weight. After papaln treatment of dellpldated dried artery, the aortic GAG were isolated, purified using cetylpyridinium chloride, and Identified and quantltated by a combination of procedures Including selective enzymatic digestion and electrophoresis. In both pigeon groups, aortic GAG Included 8% hyaluronic acid, 11% dermatan sulfate, 15% heparan sulfate, and 66% chondroltin sulfate. For the entire group, total aortic GAG content was 35% greater in WC-2 pigeons. Since we did not know if this increase in GAG was simply due to Increased atherosclerosis In the WC-2 birds, we sorted the pigeons Into matched groups representing minimal, moderate, and severe atherosclerosis on the basis of aortic cholesterol content. At all levels of cholesterol, all GAG contents were greater In the aortas of WC-2 pigeons. The accumulation of dermatan sulfate was 30% higher than in RBWC birds In the minimal arteriosclerosis group, 101% higher in the moderate group, and 53% higher in the severe group. Hyaluronic acid tended to decrease as aortic cholesterol contents increased in WC-2 pigeons. Reduced hyaluronic acid and increased dermatan sulfate may suggest the presence of an altered hyaluronic acld-dermatan sulfate-contalnlng proteoglycan aggregate In the Intercellular matrix of the WC-2 pigeon aoria. Possible consequences Include Increased artery permeability and a binding and retention of llpoprotelns In the artery wall. These factors may explain why atherosclerosis develops at an Increased rate In the WC-2 pigeon. (Arteriosclerosis 1981; 1:192-201)

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Section: Methodsmentioning

confidence: 99%

Aortic glycosaminoglycans in genetically selected WC-2 pigeons with increased atherosclerosis susceptibility.

Nohlgren¹

1981

Arteriosclerosis

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“…After cooling, Ehrlich's reagent was added and the pink color development was measured at 540 nm. 28 …”

Section: Assay Of Lysosomal Enzymes and Glycoproteinsmentioning

confidence: 99%

Protective effect of administration ofWithania somiferaagainst bromobenzene induced nephrotoxicity and mitochondrial oxidative stress in rats

2014

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Background: The present study was conducted to elucidate the protective role of Withania somnifera against bromobenzene induced nephrotoxicity and mitochondrial dysfunction in rats. Methods: In this study, Wistar albino rats of either sex were divided into six groups consisting of six animals each. The first one was control, those in group II received bromobenzene (10 mmol/kg, intragastric intubation) once, but group III and IV animals received W. somnifera (250 and 500 mg/kg, orally), respectively for 8 days followed by bromobenzene once on the 8th day and silymarin (100 mg/kg, orally) was given for 8 days to group V animals and then bromobenzene on the last day. Group VI animals received only W. somnifera (500 mg/kg) for 8 days. Results: The levels of renal lipid peroxidation, lysosomal enzymes and glycoprotein were increased significantly (p50.05) in the bromobenzene alone treated rats and antioxidant status and mitochondrial enzymes were found to be decreased, when compared to the control group. The levels of kidney functional markers (urea, uric acid and creatinine) were also found to be abnormal in serum of bromobenzene alone treated rats. On the other hand, administration of W. somnifera (250 and 500 mg/kg) along with bromobenzene offered a significant dose-dependent protection to the biochemical alterations as observed in the bromobenzene alone treated rats, which was also evidenced by histopathology. Conclusion: Thus, the oral administration of W. somnifera significantly protected against the bromobenzene induced nephrotoxicity and renal mitochondrial dysfunction in rats.

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“…Hexose was estimated by the method of Niebes (1972). Hexosamine was estimated by the method of Wagner (1979). Sialic acid was estimated by the method of Winzler (1955).…”

Section: Biochemical Analysismentioning

confidence: 99%

Effect of Luteolin on the Levels of Glycoproteins During Azoxymethane-induced Colon Carcinogenesis in Mice

Pandurangan¹,

Dharmalingam²,

Sadagopan³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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A more sensitive assay discriminating galactosamine and glucosamine in mixtures

Cited by 216 publications

References 6 publications

Aortic glycosaminoglycans in genetically selected WC-2 pigeons with increased atherosclerosis susceptibility.

Aortic glycosaminoglycans in genetically selected WC-2 pigeons with increased atherosclerosis susceptibility.

Protective effect of administration ofWithania somiferaagainst bromobenzene induced nephrotoxicity and mitochondrial oxidative stress in rats

Effect of Luteolin on the Levels of Glycoproteins During Azoxymethane-induced Colon Carcinogenesis in Mice

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