A more accurate detection of codon 72 polymorphism and LOH of the TP53 gene

Baccouche, Sami; Mabrouk, Imed; Saïd, Salem; Mosbah, A; Jlidi, R.; Gargouri, Ali

doi:10.1016/s0304-3835(02)00405-6

Cited by 8 publications

(6 citation statements)

References 16 publications

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“…For the homozygote cases, the presence of either 1126 bp or 1006 bp indicates a Pro/Pro or Arg/Arg genotype, respectively. The 280‐bp fragment is present in all cases and represents internal control of the total DNA digestion, as reported in our previous work 20…”

Section: Resultssupporting

confidence: 61%

“… Detection of codon 72 polymorphism by Acc II digestion of PCR fragment ex4‐6 of the TP53 gene in 10 blood samples (S1‐S10) from independent subjects. We focused on the doublet 1006–1126 bp fragments of Acc II digestion, because it was sufficient to conclude for the codon 72 genotype 20. The 100‐bp fragment was not shown.…”

Section: Resultsmentioning

confidence: 99%

“…However, with the previous method, partial digestion of the amplified fragment (exon 4) could cause false conclusions. Therefore, we introduced a sensitivity improvement to this method, which is based on amplification of the exon 4–6 fragment that contains an additional Acc II site 20…”

Section: Introductionmentioning

confidence: 99%

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No Evidence of Correlation between p53 Codon 72 Polymorphism and Risk of Bladder or Breast Carcinoma in Tunisian Patients

Mabrouk

Baccouche

El-Abed

et al. 2003

Annals of the New York Academy of Sciences

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The TP53 gene, frequently mutated in human cancers, carries several polymorphisms. The one most informative and studied concerns codon 72; a single base changes the CGC (arginine) to CCC (proline). The arginine form was considered to be a significant risk factor in the development of cancer. However, various reports on this polymorphism are controversial. We carried out the same investigation in two groups of patients, a group with bladder cancer and another with breast cancer, and in healthy controls in two regions of our country, using an improved PCR-RFLP method. The number of Arg/Arg, Arg/Pro, and Pro/Pro genotypes was as follows: 21, 23, 3 and 13, 19, 2 for patients (total 47) and controls (34), respectively, in the first group; 18, 9, 3 and 19, 26, 4 for patients (30) and controls (49), respectively, in the second group. Statistical analysis of the genotype and allele frequencies did not reveal any difference between patients and controls in both groups except for a weak difference between the homozygotes to heterozygotes in the second group with a chi square of 4.1 (P = 0.045); the number of breast cancer patients is actually low (30) and should be increased in order to assess such a conclusion. Our overall results are therefore not consistent with a high risk associated with TP53 codon 72 polymorphism in breast and in bladder cancers.

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Section: Resultssupporting

confidence: 61%

Section: Resultsmentioning

confidence: 99%

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No Evidence of Correlation between p53 Codon 72 Polymorphism and Risk of Bladder or Breast Carcinoma in Tunisian Patients

Mabrouk

Baccouche

El-Abed

et al. 2003

Annals of the New York Academy of Sciences

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“…Recently, Singh et al have pointed out a protective association of heterozygous variant with postmenopausal breast cancer risk [42]. In the present study, we did not observe statistically significant differences between the genotype distributions of p53 Arg72Pro and breast cancer risk as shown in previous studies [8,29,31,32,43,44]. On the other hand, we showed that the G13964C intronic variant could be associated with familial breast cancer risk in our cohort.…”

Section: Discussioncontrasting

confidence: 52%

Haplotype analysis of p53 polymorphisms: Arg72Pro, Ins16bp and G13964C in Tunisian patients with familial or sporadic breast cancer

Trifa

Karray-Chouayekh

Mabrouk

et al. 2010

Cancer Epidemiology

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“…In addition, lines representing the 99% significance borders of the normal tissue samples are included. To validate our method we performed a standard RFLP [25] on some of the samples homozygous for Arg or Pro and heterozygous with or without LOH (data not shown).…”

Section: Assessment Of Tp53 Mutations In Tumor Tissuementioning

confidence: 99%

LOH rather than genotypes ofTP53codon 72 is associated with disease-free survival in primary breast cancer

Kyndi¹,

Alsner²,

Hansen³

et al. 2006

Acta Oncologica

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A more accurate detection of codon 72 polymorphism and LOH of the TP53 gene

Cited by 8 publications

References 16 publications

No Evidence of Correlation between p53 Codon 72 Polymorphism and Risk of Bladder or Breast Carcinoma in Tunisian Patients

No Evidence of Correlation between p53 Codon 72 Polymorphism and Risk of Bladder or Breast Carcinoma in Tunisian Patients

Haplotype analysis of p53 polymorphisms: Arg72Pro, Ins16bp and G13964C in Tunisian patients with familial or sporadic breast cancer

LOH rather than genotypes ofTP53codon 72 is associated with disease-free survival in primary breast cancer

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