A Mordanting Fixation for Intense Staining of Small Chromosomes

Hyde, Beal B.; Gardella, Catherine

doi:10.3109/10520295309105561

Cited by 28 publications

(11 citation statements)

References 7 publications

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“…A few young sori were present on the plants on Gunong Poe; these were fixed in the field in a I : 3 mixture of ferric proprionate and 95 % alcohol (Hyde and Gardella, 1953). They were placed in a refrigerator on reaching England some months later and subsequently squashed and stained with aceto-carmine.…”

Section: [Tuith Elizabeth W Davies)mentioning

confidence: 99%

THE MORPHOLOGY AND CYTOLOGY OF SPOROGENESIS OF TRICHOMANES PROLIFERUM BL.

Bell

1960

New Phytologist

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Summary The morphology of Trichomanes proliferum is described and the anatomy of the petiole and rhizome is discussed in relation to the habitat of the plant and its growth. It is suggested that after the initial phases each frond may be regarded as a more or less independent colony of cells, the complexity ultimately attained by the frond depending upon the supply of primary metabolites reaching it directly from without. It is reported that in the plant collected from Gunong Poe the chromosomes failed to pair at meiosis and 108 univalents were present. A means is suggested by which polyploidy could have arisen frequently in the Hymenophyllaceae.

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Section: [Tuith Elizabeth W Davies)mentioning

confidence: 99%

THE MORPHOLOGY AND CYTOLOGY OF SPOROGENESIS OF TRICHOMANES PROLIFERUM BL.

Bell

1960

New Phytologist

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“…distilled water) recommended by Johansen (1940, p. 235) and used by Cave and Pocock (1956). The second fixative (one part of a solution containing 0.4 per cent ferric hydroxide dissolved in propionic acid to two parts of absolute ethanol) was used as a mordant (Hyde and Gardella, 1953). A drop or two of this mordanting fixative was added to the drop of the first fixative containing the organisms and allowed to act for 2-5 min.…”

mentioning

confidence: 99%

A Morphological Study of Astrephomene Gubernaculifera and Volvulina Steinii

Stein

1958

American Journal of Botany

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“…These cells have a prochromosomal type of nucleus with usually 1 or 1 large and 1 small nu-cleolus. Unlike P. ovata (Hyde, 1953), the early pachytene chromosomes are not very conspicuously differentiated. The centromeric heterochromatin becomes more conspicuous at late pachytene and early diplotene ( fig.…”

mentioning

confidence: 85%

“…For cytological studies, the young spikes were fixed in Carnoy's fluid (6 alcohol : 3 chloroform : 1 acetic acid/propionic acid) mordanted with Fe (0H) 3, as described by Hyde and Gardella (1953) . The anthers were squashed in acetocarmine.…”

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confidence: 99%

The Association of a Single B‐chromosome With Male Sterility in Plantago Coronopus

Paliwal

Hyde

1959

American J of Botany

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PALIWAL, RIPSUDAN L. (B. R. College, Agra, India.}, and BEAL B. HYDE. The association of a single B-chromosome with male sterility in Plantago coronopus. Amer. Jour. Bot. 46 (6) : 460-466. Illus. 1959.-Two species of Plantago showing male sterility have been studied cytogenetically. In P. ovata (n=4) the sterility appears to be cytoplasmic, In P. coronopus (n=5) all malesterile plants contain a single extra chromosome which is largely heterochromatic, shorter, and not homologous with any of the other chromosomes. No male-fertile plants contain this B-chromosome. Meiosis is regular in the male-sterile lines. The accessory chromosome usually does not divide and moves to one pole at the first division of meiosis and divides regularly in the second division. Degeneration of all microspores occurs before pollen mitosis. Male-sterile plants are apomictic, hut whether or not male-fertile plants are also apomictic has not yet been determined.

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A Mordanting Fixation for Intense Staining of Small Chromosomes

Cited by 28 publications

References 7 publications

THE MORPHOLOGY AND CYTOLOGY OF SPOROGENESIS OF TRICHOMANES PROLIFERUM BL.

THE MORPHOLOGY AND CYTOLOGY OF SPOROGENESIS OF TRICHOMANES PROLIFERUM BL.

A Morphological Study of Astrephomene Gubernaculifera and Volvulina Steinii

The Association of a Single B‐chromosome With Male Sterility in Plantago Coronopus

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