A mononuclear Ni(II) complex with 5,6-diphenyl-3-(2-pyridyl)-1,2,4-triazine: DNA- and BSA-binding and anticancer activity against human breast carcinoma cells

In vitro DNA and BSA-binding, cell imaging and anticancer activity against human carcinoma cell lines of mixed ligand copper(II) complexes

“…A high K SV value of 1.77 Â 10 5 M À1 for the complex (1) and relatively high K SV value of 0.38 Â 10 5 M À1 for the complex (2) was calculated, which illustrates the complex (1) has a high quenching efficiency and can compete for DNA-binding sites with EB [34]. This K SV is greater than or similar to the values obtained for other complexes with N-donar ligands: (Cu(II): K SV = 2.45 Â 10 4 M À1 ) [32], (Zn(II): K SV = 3.10 Â 10 4 M À1 ) [22] and (Ni(II): K SV = 8.8 Â 10 5 M À1 ) [23]. Moreover, to estimate the strength of the binding of the complex (1) with DNA the apparent binding constant (K app ) is calculated using the equation K EB [EB] = K app [Complex], where [EB] = 1.0 lM, K EB = 1.0 Â 10 7 M À1 [35], and the complex concentration was the value at 50% reduction of the fluorescence intensity of EB.…”

“…In continuous of our research [22,23], consideration the effect factors on the medicinal properties of metal complexes, the biological and anticancer properties of two mononuclear Cu(II) complexes with the polypyridyl ligands, [Cu(phen-dion)(phen)Cl]Cl (1) and [Cu(phen-dione)(bpy)Cl]Cl (2), (Fig. 1), have been investigated.…”

Competitive DNA-Binding Studies between Metal Complexes and GelRed as a New and Safe Fluorescent DNA Dye

“…These results show complexes 5 and 6 cannot compete for DNA-binding sites with GR and their interaction with DNA is external binding. Obtained results from competitive DNA-binding studies between metal complexes with EB [16,[20][21][22] are similar to obtained results with GR (Tables 1 and 2).…”

“…Table 1 indicates obtained results from quenching of the emission band of the GR-DNA system at 592 nm with the addition of complexes. Table 2 shows obtained results from quenching of the emission band of the EB-DNA system at 592 nm with the addition of complexes in our previous reported [16,[20][21][22]. Furthermore, the quenching constants (K SV ) are calculated according to the Stern-Volmer equation (Eq.…”

A mononuclear Cu(II) complex with 5,6-diphenyl-3-(2-pyridyl)-1,2,4-triazine: Synthesis, crystal structure, DNA- and BSA-binding, molecular modeling, and anticancer activity against MCF-7, A-549, and HT-29 cell lines

“…Dppt (5,6-diphenyl-3-(2-pyridyl) -1,2,4-triazine) is an important derivative of 1,2,4-triazine which has three rings (two phenyl and one pyridyl ring) attached to the triazine ring. We have previously studied the interaction of [Zn(dppt) 2 Cl 2 ] and [Ni(dppt) 2 Cl 2 ] with DNA and BSA [26,27] …”