A monokaryotization method and its use for genetic studies in wood-rotting basidiomycetes

Leal-Lara, Hermilo; Eger-Hummel, G.

doi:10.1007/bf00261513

Cited by 22 publications

(11 citation statements)

References 9 publications

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“…The chemical dedikaryotization method proposed by Leal-Lara and Eger-Hummel (1982) was used for obtaining the neohaplonts. A PGS solution was employed as the dedikaryotization medium and the following parameters were varied: homogenization time (Ht), volume of inoculum (Vi) and incubation time (It).…”

Section: Methodsmentioning

confidence: 99%

Morphology and mycelial growth rate of Pleurotus spp. strains from the Mexican mixtec region

Guadarrama-Mendoza¹,

Toro²,

Ramírez-Carrillo³

et al. 2014

Braz. J. Microbiol.

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Two native Pleurotus spp. strains (white LB-050 and pale pink LB-051) were isolated from rotten tree trunks of cazahuate (Ipomoea murucoides) from the Mexican Mixtec Region. Both strains were chemically dedikaryotized to obtain their symmetrical monokaryotic components (neohaplonts). This was achieved employing homogenization time periods from 60 to 65 s, and 3 day incubation at 28 °C in a peptone-glucose solution (PGS). Pairing of compatible neohaplonts resulted in 56 hybrid strains which were classified into the four following hybrid types: (R1-nxB1-n, R1-nxB2-1, R2-nxB1-n and R2-nxB2-1). The mycelial growth of Pleurotus spp. monokaryotic and dikaryotic strains showed differences in texture (cottony or floccose), growth (scarce, regular or abundant), density (high, regular or low), and pigmentation (off-white, white or pale pink). To determine the rate and the amount of mycelium growth in malt extract agar at 28 °C, the diameter of the colony was measured every 24 h until the Petri dish was completely colonized. A linear model had the best fit to the mycelial growth kinetics. A direct relationship between mycelial morphology and growth rate was observed. Cottony mycelium presented significantly higher growth rates (p < 0.01) in comparison with floccose mycelium. Thus, mycelial morphology can be used as criterion to select which pairs must be used for optimizing compatible-mating studies. Hybrids resulting from cottony neohaplonts maintained the characteristically high growth rates of their parental strains with the hybrid R1-nxB1-n being faster than the latter.

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Section: Methodsmentioning

confidence: 99%

Morphology and mycelial growth rate of Pleurotus spp. strains from the Mexican mixtec region

Guadarrama-Mendoza¹,

Toro²,

Ramírez-Carrillo³

et al. 2014

Braz. J. Microbiol.

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“…The production of hybrid and reconstituted strains (modified strains by genetic engineering) not only increase the quality of cultivated mushrooms and reduce cultivation costs, but they can also rise farmer's revenue in the short term (Avin et al, 2012. In some cases these new strains are produced through paring compatible monokaryons recovered by chemical dedikaryotization, this process allows the recovery of the two neohaplonts from the dikaryotic strain (Lara and Eger-Hummel, 1982).…”

Section: Introductionmentioning

confidence: 99%

MODELING OF MYCELIAL GROWTH OF PARENTAL, HYBRID AND RECONSTITUTED STRAINS OF Pleurotus AND Lentinula

et al. 2019

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“…Thereafter, 50 mL were poured into glass jars and sterilized at 15 psi (121 °C) for 15 min. Then, the glass jars with the dedikaryotization solution were incubated at 28 °C for 24 h to check the sterility (Leal-Lara and Eger-Hummel, 1982).…”

Section: Dedikaryotization Solution (Peptone-glucose Solution Pgs)mentioning

confidence: 99%

“…This process allows the recovery of the two monokaryotic components from a dikaryon (LealLara and Eger-Hummel, 1982) by using toxic substances such as sodium taurocholate, colic acid, peptone or glucose (Miles and Raper, 1956). …”

Section: Introductionmentioning

confidence: 99%

Production of hybrid strains among Pleutorus and Lentinula and evaluation of their mycelial growth kinetics on malt extract agar and wheat grain using the Gompertz and Hill models

et al. 2018

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Three inter-generic hybrid strains were obtained by pairing compatible neohaplonts recovered by dedikaryotization of four parental strains, i.e. Pleurotus ostreatus (CC060), Pleurotus djamor (CC051) and Lentinula edodes (CC003 and CC004). Fifteen neohaplonts were recovered employing homogenization time periods for Pleurotus spp. since 60 to 90 s, and for Lentinula edodes strains time periods ranged from 5 to 20 s, and incubation at 28 °C in a peptone-glucose solution (PGS). The mycelial growth of the parental and hybrid strains was determined on malt extract agar (MEA) and wheat grain by calculating the parameters A, B and C of nonlinear regression models. For the parameter A, the mycelial growth on MEA showed values ranged from 1.08 to 1.28, and for the parameter B since 5.65 to 20.85. On the other hand, the A values by mycelium growth on wheat grain ranged from 1.57 to 16.13, for B since 28.36 to 86.53 and for C were in an interval from 11.89 to 44.24. Parental and hybrid strains presented instantaneous velocity values on MEA since 2.88 to 7.26 cm2•day-1, whereas on wheat grain were in an interval from 5.45 to 10.05 cm3•day-1. Furthemore, the µmax values on MEA and wheat grain were calculated by using the Gompertz model and the λ values on both medium were estimated by using the Hill model, the µmax values on MEA ranged from 0.26 to 1.69 day-1 and λ values in an interval from 0.41 to 2.74, whereas the µmax values on wheat grain ranged from 0.05 to 0.43 day-1 and λ since 1.82 to 28 h. Moreover, the estimated equations based on nonlinear models were used to calculate the µmax values on MEA and wheat grain of the strains and the λ values were obtained by using the µmax values of the proposed equations on the Hill model, the µmax values on MEA ranged from 0.44 to 1.27 day-1 and the λ values between 0.57 to 1.72 h, while the µmax values on wheat grain ranged from 0.04 to 0.64 day-1 and λ values in an interval from 1.26 to 20.11 h. The results evidenced that the hybrid strains of PleurotusxLentinula presented highest rate of growth in comparison with the parental, encouraging the production of hybrid strain and their use in the industrial field.

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A monokaryotization method and its use for genetic studies in wood-rotting basidiomycetes

Cited by 22 publications

References 9 publications

Morphology and mycelial growth rate of Pleurotus spp. strains from the Mexican mixtec region

Morphology and mycelial growth rate of Pleurotus spp. strains from the Mexican mixtec region

MODELING OF MYCELIAL GROWTH OF PARENTAL, HYBRID AND RECONSTITUTED STRAINS OF Pleurotus AND Lentinula

Production of hybrid strains among Pleutorus and Lentinula and evaluation of their mycelial growth kinetics on malt extract agar and wheat grain using the Gompertz and Hill models

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