A Monocyte‐Aggregating Factor in the Sera of Patients with Sarcoidosis

Abstract

Sarcoidosis sera were shown to have higher activity in aggregating normal human monocytes than normal human sera, A kinetic study suggested that monocytes may not be sensitive to the aggregating activity of sarcoidosis serum until they develop to some extent into macrophages. Gel filtration of sarcoidosis sera on Sephadex G-200 showed that the major factor responsible for monocyte aggregation had the molecular weight of about 130,000, This major aggregating factor was unable to increase cell size of normal human monocytes or to increase phagocytosis and glucose consumption of normal human monocytes. It did have the ability to form multinucleate giant cells,

Monocyte‐modulating Activities in the Sera of Patients with Granuloma Annulare

“…In our previous study, we found that the percentage of aggregated monocytes in sarcoidosis sera was significantly higher than that in normal human sera from 24 hr after incubation, but not earlier (4). In the present study, the cell aggregation with granuloma annulare sera also showed a similar time lag.…”

“…In our previous study, the monocytes incubated with sarcoidosis sera showed obvious aggregation after 24 hr incubation (4). Thus, in this study, after estimation of spreading, the cells were further incubated with test samples, respectively.…”

“…In our previous study, we found that sarcoidosis sera could induce normal human monocytes to spread (3) and to aggregate (4). Thus, in the present study, attempts were made to determine whether the sera from patients with granuloma annulare also had the similar monocyte modulating activities.…”

“…Based on the overlap of clinical and histopathological findings between granuloma annulare and sarcoidosis as well as macrophage migration inhibitory activity in the sera of these two diseases, Umbert and Winkelmann have postulated that a similar pathogenic mechanism may be involved in both granuloma annulare and sarcoidosis (1). In our previous study, gel filtration of sarcoidosis sera on Sephadex G-200 showed that the most obvious spreading activity eluted at a position with a molecular weight of about 70,000 (3) and the most obvious aggregating activity eluted at a position with a molecular weight of about 130,000 (4). Similar column chromatography of granuloma annulare sera revealed that peaks of these activities were respectively found around the corresponding positions as well.…”

Effect of Human Fibroblast Interferon on Normal Human Monocyte Activation Induced by a Factor Found in Sarcoidosis Sera

“…In our previous study, we demonstrated that sarcoidosis sera contains a factor which induces normal human monocytes to spread (1) as well as another which aggregates normal human monocytes (2). Spreading of mononuclear phagocytes is viewed· as a morphological expression of their activation (3).…”